FastQCFastQC Report
Thu 2 Feb 2017
SRR4064094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331976
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15750.11824537379051875No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15070.11314017669988047No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14350.1077346738980282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG6000.023.0990924
CGTCGTA4500.022.48800710
GTATCAA22950.022.049551
CCCTATA1004.6267814E-821.9991382
CCGTCGT4400.020.9983889
TTAGGAC4900.020.2032873
ATACCGT4800.020.1658766
AGGACGT6700.019.3723755
ACCGTCG4900.019.304648
TGTAGGA7750.019.3024672
TATACTG2200.018.9992545
GTCTTAC1051.7917082E-618.8585261
GGACGTG6700.018.7156856
TACCGTC4950.018.6659367
GTCGTAG5100.018.54829211
GACGTGA4000.018.1492887
GTAGGAC8550.018.010993
CTTTAGG5350.017.683541
AACGCAG28550.017.2602676
CGTGAAA5000.017.1586829
Produced by FastQC (version 0.11.5)