FastQCFastQC Report
Thu 2 Feb 2017
SRR4064093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702009
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31220.4447236431441762No Hit
TATCAACGCAGAGTACTTTTTTTTT23460.3341837497809857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21070.30013860221165256No Hit
GTACATGGGGTGGTATCAACGCAAA15330.2183732687187771No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14930.21267533607119No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13420.1911656403265485No Hit
GGTATCAACGCAGAGTACTTTTTTT11010.156835596124836No Hit
GTATCAACGCAGAGTACATGGGGTG8940.12734879467357257No Hit
GTACATGGGTGGTATCAACGCAAAA7760.11053989336319049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA504.58499E-617.0882662
GTATTAG702.521665E-816.272221
GTCATAG601.4767193E-615.8202151
CGAACGT508.727625E-515.19498354
TCGAACG508.743557E-515.1917343
AACCGCG400.005271868314.25138957
TACTACA602.580954E-514.2402222
AACGTCT551.9596721E-413.8165746
ATTCGAA551.9715558E-413.8067321
AAGACGA500.001499430613.2984526
TCTATAC802.0071693E-613.0553973
CGGTTCT1102.7248461E-912.95765812
GTCCTAA2100.012.6561721
GTAGGAC3150.012.35835553
TAATACT1001.444223E-712.3459244
AGGCCCG853.924053E-612.297070510
CCGAGCC853.9271836E-612.29619314
CCGTCGT701.0868153E-412.217229
TAAGGCT701.0926183E-412.2102544
CCGACCA1103.787318E-812.0938149
Produced by FastQC (version 0.11.5)