##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064093_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702009
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16194521722656	32.0	32.0	32.0	32.0	32.0
2	30.7524262509455	32.0	32.0	32.0	32.0	32.0
3	30.781471462616576	32.0	32.0	32.0	32.0	32.0
4	30.796548192402092	32.0	32.0	32.0	32.0	32.0
5	30.70211065670098	32.0	32.0	32.0	32.0	32.0
6	34.343719240066726	36.0	36.0	36.0	32.0	36.0
7	34.25354803143549	36.0	36.0	36.0	32.0	36.0
8	34.19349894374574	36.0	36.0	36.0	32.0	36.0
9	34.31550307759587	36.0	36.0	36.0	32.0	36.0
10	34.0104115474303	36.0	36.0	36.0	32.0	36.0
11	34.3721362546634	36.0	36.0	36.0	32.0	36.0
12	34.14989551416007	36.0	36.0	36.0	32.0	36.0
13	34.24002683726277	36.0	36.0	36.0	32.0	36.0
14	34.143045174634516	36.0	36.0	36.0	32.0	36.0
15	34.08123400127349	36.0	36.0	36.0	32.0	36.0
16	34.076103012924335	36.0	36.0	36.0	32.0	36.0
17	33.99684619427956	36.0	36.0	36.0	32.0	36.0
18	33.998689475491055	36.0	36.0	36.0	32.0	36.0
19	34.02229885941633	36.0	36.0	36.0	32.0	36.0
20	33.97224394559044	36.0	36.0	36.0	32.0	36.0
21	33.95245644998853	36.0	36.0	36.0	32.0	36.0
22	33.94322152564995	36.0	36.0	36.0	32.0	36.0
23	33.89743436337711	36.0	36.0	36.0	32.0	36.0
24	33.89776199450434	36.0	36.0	36.0	32.0	36.0
25	33.48636698389907	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	3.0
5	7.0
6	29.0
7	13.0
8	39.0
9	41.0
10	62.0
11	19.0
12	48.0
13	35.0
14	81.0
15	146.0
16	217.0
17	282.0
18	362.0
19	502.0
20	790.0
21	1191.0
22	1835.0
23	3032.0
24	4239.0
25	6253.0
26	8713.0
27	11448.0
28	15558.0
29	20577.0
30	26841.0
31	36297.0
32	50115.0
33	70062.0
34	145866.0
35	297304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75408915628999	17.52473159345279	11.765426785648122	24.955752464609095
2	15.870734295869452	20.276224598092497	39.08627923044597	24.76676187559209
3	18.588123680559317	25.463460218062057	28.941797886615706	27.006618214762923
4	11.948454518776835	15.922630494799012	37.037986729004444	35.090928257419705
5	13.573454557369388	37.92714876109586	33.99097657813722	14.508420103397532
6	32.49354272061956	36.57106711439037	17.82565274439716	13.109737420592914
7	29.152685818825308	31.2266243786092	21.410323814246002	18.21036598831949
8	27.92961736424211	33.320176013588615	19.74923193552248	19.000974686646792
9	27.73931409547481	13.884122495981027	18.951304883193288	39.42525852535088
10	15.46402625964034	27.600415616105266	32.40636878945396	24.52918933480044
11	36.19861520086429	21.50044611170181	23.41958159568312	18.88135709175078
12	24.70341562008692	24.161916612858857	29.942514727661205	21.19215303939302
13	29.70230841048228	20.363473092300787	25.70609993371961	24.228118563497326
14	23.29566916731642	20.259396679642677	26.644819834908652	29.800114318132252
15	24.373032151210605	28.29825354088066	23.6101945435446	23.71851976436414
16	24.29134677610179	26.28167664943795	25.587919183740958	23.8390573907193
17	22.795404658057528	26.54194817412127	26.380455543202487	24.28219162461872
18	23.416733549353722	25.722012268399393	28.002628739074837	22.858625443172052
19	24.923235266445616	25.244406937194046	26.692601789894137	23.1397560064662
20	25.27877172224044	24.75829329835916	26.43733873152095	23.525596247879456
21	26.35339331942021	24.322448025362938	25.530437807200613	23.79372084801624
22	25.12566539219292	25.23686108806414	25.91358668844962	23.72388683129332
23	23.615679062506683	25.285517752639198	26.683774208954375	24.415028975899748
24	24.104847941403303	25.848749702411926	26.52375786380944	23.52264449237533
25	24.134671859585897	25.426534068553373	26.656526028272136	23.78226804358859
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	111.0
2	330.5
3	550.0
4	550.0
5	550.0
6	1552.0
7	2554.0
8	2554.0
9	2554.0
10	2500.5
11	2447.0
12	2447.0
13	2447.0
14	2350.5
15	2254.0
16	2254.0
17	2254.0
18	3890.5
19	5527.0
20	5527.0
21	5527.0
22	9167.0
23	12807.0
24	12807.0
25	12807.0
26	18948.5
27	25090.0
28	25090.0
29	25090.0
30	31737.5
31	38385.0
32	38385.0
33	38385.0
34	44834.0
35	51283.0
36	51283.0
37	51283.0
38	56623.5
39	61964.0
40	61964.0
41	61964.0
42	69919.5
43	77875.0
44	77875.0
45	77875.0
46	86214.5
47	94554.0
48	94554.0
49	94554.0
50	93692.0
51	92830.0
52	92830.0
53	92830.0
54	83761.0
55	74692.0
56	74692.0
57	74692.0
58	67163.0
59	59634.0
60	59634.0
61	59634.0
62	51816.5
63	43999.0
64	43999.0
65	43999.0
66	35602.0
67	27205.0
68	27205.0
69	27205.0
70	20384.0
71	13563.0
72	13563.0
73	13563.0
74	10341.0
75	7119.0
76	7119.0
77	7119.0
78	5669.5
79	4220.0
80	4220.0
81	4220.0
82	2889.0
83	1558.0
84	1558.0
85	1558.0
86	1195.0
87	832.0
88	832.0
89	832.0
90	603.5
91	375.0
92	375.0
93	375.0
94	271.5
95	168.0
96	168.0
97	168.0
98	290.5
99	413.0
100	413.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03917328695216159
2	0.0062677259123458525
3	9.971382133277494E-4
4	0.003846104537121319
5	0.008262002339001352
6	0.012535451824691705
7	0.02222193732558984
8	0.034899837466471226
9	0.048005082555921647
10	0.056979326475871395
11	0.055697291630164285
12	0.06239236249107917
13	0.06324705238821725
14	0.06524132881487274
15	0.05954339616728561
16	0.06652336366057984
17	0.06111032764537207
18	0.07578250421290895
19	0.07336088283768442
20	0.07677964242623671
21	0.07307598620530506
22	0.07734943569099542
23	0.08034085033097867
24	0.07635229747766767
25	0.07706453905861606
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	702009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.034889597737795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54527247284346	45.003313267879655
2	14.010336681595895	16.26176685087108
3	4.181757508124178	7.280635059254934
4	1.6343908305668238	3.7940676564600233
5	0.8023736004722161	2.3282831659772216
6	0.43277231441898817	1.506953609295807
7	0.2616343004564472	1.062874241937989
8	0.18800601699698183	0.8728726752104204
9	0.12673970149576944	0.6619792125561501
>10	0.6602324175452297	7.1879222047522315
>50	0.08304761617528436	3.4441231488908146
>100	0.06900077518950179	7.765634258941619
>500	0.0027107447394920246	0.9640719081111849
>1k	0.0017250193796767431	1.8655027398608957
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3122	0.4447236431441762	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2346	0.3341837497809857	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2107	0.30013860221165256	No Hit
GTACATGGGGTGGTATCAACGCAAA	1533	0.2183732687187771	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1493	0.21267533607119	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1342	0.1911656403265485	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1101	0.156835596124836	No Hit
GTATCAACGCAGAGTACATGGGGTG	894	0.12734879467357257	No Hit
GTACATGGGTGGTATCAACGCAAAA	776	0.11053989336319049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.84896632379357E-4
2	0.0	0.0	0.0	0.0	2.84896632379357E-4
3	0.0	0.0	0.0	0.0	2.84896632379357E-4
4	0.0	0.0	0.0	0.0	2.84896632379357E-4
5	0.0	0.0	0.0	0.0	2.84896632379357E-4
6	0.0	0.0	0.0	0.0	2.84896632379357E-4
7	0.0	0.0	0.0	0.0	2.84896632379357E-4
8	0.0	0.0	0.0	0.0	2.84896632379357E-4
9	0.0	0.0	0.0	0.0	2.84896632379357E-4
10	0.0	0.0	0.0	0.0	2.84896632379357E-4
11	0.0	0.0	0.0	1.424483161896785E-4	2.84896632379357E-4
12	0.0	0.0	0.0	1.424483161896785E-4	2.84896632379357E-4
13	0.0	0.0	0.0	1.424483161896785E-4	4.2734494856903546E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTATA	50	4.58499E-6	17.088266	2
GTATTAG	70	2.521665E-8	16.27222	1
GTCATAG	60	1.4767193E-6	15.820215	1
CGAACGT	50	8.727625E-5	15.1949835	4
TCGAACG	50	8.743557E-5	15.191734	3
AACCGCG	40	0.0052718683	14.2513895	7
TACTACA	60	2.580954E-5	14.240222	2
AACGTCT	55	1.9596721E-4	13.816574	6
ATTCGAA	55	1.9715558E-4	13.806732	1
AAGACGA	50	0.0014994306	13.298452	6
TCTATAC	80	2.0071693E-6	13.055397	3
CGGTTCT	110	2.7248461E-9	12.957658	12
GTCCTAA	210	0.0	12.656172	1
GTAGGAC	315	0.0	12.3583555	3
TAATACT	100	1.444223E-7	12.345924	4
AGGCCCG	85	3.924053E-6	12.2970705	10
CCGAGCC	85	3.9271836E-6	12.296193	14
CCGTCGT	70	1.0868153E-4	12.21722	9
TAAGGCT	70	1.0926183E-4	12.210254	4
CCGACCA	110	3.787318E-8	12.093814	9
>>END_MODULE