FastQCFastQC Report
Thu 2 Feb 2017
SRR4064093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702009
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10220.14558217914585142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.12620920814405515No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA7130.10156564944324076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA300.0057459429.33067140
TATGTCG407.029974E-427.49946616
CCAAGCG450.001398368624.442239
GACGTGA2650.024.0732887
TAGGACG3600.023.2201184
TTAGGAC3150.023.045533
CAAGCGC500.002580728421.99800710
CAATACT803.6148122E-621.9980054
ACGACGG1500.021.9980056
CGTTTAT1451.2732926E-1121.24550831
GATTTCG1800.020.77589441
ATTTCGT1850.020.21438242
ACCCTAT550.00448412419.9981861
AACGATT550.00448412419.99818639
GTGTAGA2000.019.7982061
AGGACGT4300.019.4400985
GATATAC2150.019.4400981
CGACGGT1705.456968E-1219.4100067
CGTGAAA3400.019.4100069
TACCTTG808.987989E-519.2482557
Produced by FastQC (version 0.11.5)