##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702009
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236670754933343	32.0	32.0	32.0	32.0	32.0
2	31.407415004650936	32.0	32.0	32.0	32.0	32.0
3	31.49556344719227	32.0	32.0	32.0	32.0	32.0
4	31.571257633449143	32.0	32.0	32.0	32.0	32.0
5	31.5226813331453	32.0	32.0	32.0	32.0	32.0
6	35.0998363268847	36.0	36.0	36.0	36.0	36.0
7	35.116528420575804	36.0	36.0	36.0	36.0	36.0
8	35.0826542109859	36.0	36.0	36.0	36.0	36.0
9	35.182021882910334	36.0	36.0	36.0	36.0	36.0
10	35.036208937492255	36.0	36.0	36.0	36.0	36.0
11	35.186984782246384	36.0	36.0	36.0	36.0	36.0
12	35.09646457524049	36.0	36.0	36.0	36.0	36.0
13	35.13923753114276	36.0	36.0	36.0	36.0	36.0
14	35.08145906961307	36.0	36.0	36.0	36.0	36.0
15	35.061517729829674	36.0	36.0	36.0	36.0	36.0
16	35.07926679002691	36.0	36.0	36.0	36.0	36.0
17	35.040002336152384	36.0	36.0	36.0	36.0	36.0
18	35.03707787222101	36.0	36.0	36.0	36.0	36.0
19	35.02542132650721	36.0	36.0	36.0	36.0	36.0
20	35.01929747339422	36.0	36.0	36.0	36.0	36.0
21	35.01103119760573	36.0	36.0	36.0	36.0	36.0
22	34.99951282675863	36.0	36.0	36.0	36.0	36.0
23	34.95454046885439	36.0	36.0	36.0	36.0	36.0
24	34.93416323722345	36.0	36.0	36.0	36.0	36.0
25	34.909390050554904	36.0	36.0	36.0	32.0	36.0
26	34.845274063437934	36.0	36.0	36.0	32.0	36.0
27	34.84999622511962	36.0	36.0	36.0	32.0	36.0
28	34.818690358670615	36.0	36.0	36.0	32.0	36.0
29	34.784213592703225	36.0	36.0	36.0	32.0	36.0
30	34.76665541324969	36.0	36.0	36.0	32.0	36.0
31	34.744725494972286	36.0	36.0	36.0	32.0	36.0
32	34.704046529318	36.0	36.0	36.0	32.0	36.0
33	34.67664089776627	36.0	36.0	36.0	32.0	36.0
34	34.6474945477907	36.0	36.0	36.0	32.0	36.0
35	34.60357630742626	36.0	36.0	36.0	32.0	36.0
36	34.56430045768644	36.0	36.0	36.0	32.0	36.0
37	34.54036629160025	36.0	36.0	36.0	32.0	36.0
38	34.49396945053411	36.0	36.0	36.0	32.0	36.0
39	34.466800283187254	36.0	36.0	36.0	32.0	36.0
40	34.43398019113715	36.0	36.0	36.0	32.0	36.0
41	34.41668554106856	36.0	36.0	36.0	32.0	36.0
42	34.33734752688356	36.0	36.0	36.0	32.0	36.0
43	34.316168311232474	36.0	36.0	36.0	32.0	36.0
44	34.23931317119866	36.0	36.0	36.0	32.0	36.0
45	34.18751184101628	36.0	36.0	36.0	32.0	36.0
46	34.13384586237498	36.0	36.0	36.0	32.0	36.0
47	34.078963375113425	36.0	36.0	36.0	32.0	36.0
48	34.018058173043364	36.0	36.0	36.0	32.0	36.0
49	33.9813150543654	36.0	36.0	36.0	32.0	36.0
50	33.40308315135561	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	0.0
20	8.0
21	21.0
22	67.0
23	237.0
24	590.0
25	1351.0
26	2629.0
27	5125.0
28	8621.0
29	12925.0
30	18359.0
31	25706.0
32	36853.0
33	58690.0
34	119753.0
35	411071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2739370075375	18.351124071702397	12.198467997327471	25.176470923432632
2	15.119451056476969	21.126975240846548	38.37818356901609	25.375390133660396
3	17.769037695049303	25.923572867313773	29.248349719801368	27.05903971783556
4	11.667656682464186	16.639245365800154	36.9952521976214	34.69784575411426
5	13.219061294085973	38.20777226502794	33.868511657257955	14.70465478362813
6	32.611451721347905	36.87550569224282	17.515754806213035	12.997287780196237
7	28.971892058210162	31.54337272509715	21.414428325603126	18.07030689108956
8	27.84166584758885	33.48219182375154	19.655446012800407	19.020696315859198
9	27.813572384006246	13.759563760062335	18.88635168995967	39.54051216597175
10	15.335699918518053	27.94916210348777	32.459444213357344	24.255693764636835
11	36.3865705425429	21.391178745571636	23.323490154684627	18.898760557200834
12	24.484336360839194	24.416958211302436	29.90820617428861	21.19049925356976
13	29.847593243903514	20.337092576527454	25.70957673970054	24.10573743986849
14	23.157713302412507	20.210595890645124	26.75995145354469	29.87173935339768
15	24.490234903631105	28.239006253650338	23.589367369905553	23.681391472813004
16	24.304277468504452	26.366231531444267	25.563672001868937	23.765818998182347
17	22.93218463011158	26.535984581394256	26.37430574251897	24.157525045975195
18	23.432169286543544	25.571185390417444	28.206494255738917	22.79015106730009
19	24.952244201997413	25.076174237082434	26.854214119762	23.117367441158162
20	25.286395195455285	24.564317480576978	26.789097466958783	23.360189857008955
21	26.292967753974665	24.213792130870118	25.87901294712746	23.61422716802776
22	25.1469710502287	25.1263160443812	26.263765849155778	23.46294705623432
23	23.640999707979287	25.100960819367383	26.892258602146708	24.36578087050662
24	24.180881902550553	25.487995099749998	26.77074949608621	23.560373501613235
25	24.206086350796372	25.131588881433963	26.900684760761084	23.761640007008577
26	23.538185392538143	26.042423465390257	27.261848796957132	23.15754234511446
27	24.15330174567854	25.727745946361768	26.378350964867042	23.740601343092646
28	23.39315909359628	25.74649243642652	26.910053377360992	23.9502950926162
29	23.671461111435583	25.473758285339027	26.78730414690745	24.06747645631794
30	23.437528935968352	25.845286410885766	27.039905638471694	23.677279014674184
31	24.103676752365903	25.4906681520505	26.37919170996266	24.026463385620932
32	23.806240598076307	25.966118885900535	25.991190682408714	24.23644983361444
33	23.469470613123484	25.484391627478942	26.74585412621463	24.300283633182946
34	24.06131676339114	25.702641333116805	26.784559412188706	23.451482491303345
35	24.4663029387883	25.71546603227966	26.68029459109104	23.137936437840995
36	23.622612587842948	26.09282825448065	26.458374586694738	23.826184570981663
37	24.265549077119662	26.109030509111992	25.98837300405848	23.637047409709865
38	23.81971314114436	26.297389550223095	26.165043564480563	23.717853744151988
39	24.25165353478228	25.881469726388605	26.088455385544624	23.77842135328449
40	24.74287456267308	25.895451800020513	26.132064183067616	23.229609454238794
41	23.644765820206214	26.258650506708147	26.716781911786914	23.379801761298722
42	24.548250481130154	26.83573767829594	25.889004591213872	22.72700724936004
43	23.811070417679847	25.963073916270297	26.25196403973236	23.973891626317492
44	23.62981354780535	26.58777811490834	25.74490422405602	24.037504113230284
45	23.780918627244287	26.668509011344664	25.719369120403872	23.831203241007174
46	23.661504755691233	26.144407201699142	25.748396369805743	24.445691672803886
47	24.30733026208201	26.083271366991738	25.914608956771712	23.69478941415454
48	24.56513223028654	26.458165656450543	25.06983667974843	23.906865433514486
49	24.053355669324763	26.74165178075401	25.233687461360727	23.971305088560495
50	23.936683941071394	27.0677291517688	25.071011193700304	23.924575713459507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	20.0
2	20.0
3	62.5
4	105.0
5	128.0
6	151.0
7	169.0
8	187.0
9	261.0
10	335.0
11	527.5
12	720.0
13	1303.5
14	1887.0
15	2670.5
16	3454.0
17	3818.0
18	4182.0
19	4230.5
20	4279.0
21	4466.5
22	4654.0
23	5143.5
24	5633.0
25	5687.5
26	5742.0
27	6943.0
28	8144.0
29	9537.5
30	10931.0
31	13061.5
32	15192.0
33	17938.0
34	20684.0
35	24105.0
36	27526.0
37	29824.0
38	32122.0
39	33322.0
40	34522.0
41	36358.5
42	38195.0
43	38740.5
44	39286.0
45	42541.0
46	45796.0
47	48600.5
48	51405.0
49	52140.5
50	52876.0
51	50636.5
52	48397.0
53	47000.5
54	45604.0
55	45100.5
56	44597.0
57	42585.0
58	40573.0
59	36754.5
60	32936.0
61	28703.0
62	24470.0
63	21174.0
64	17878.0
65	15083.5
66	12289.0
67	10544.5
68	8800.0
69	7984.5
70	7169.0
71	5565.0
72	3961.0
73	3328.0
74	2695.0
75	2128.0
76	1561.0
77	1394.5
78	1228.0
79	993.0
80	758.0
81	612.0
82	466.0
83	384.0
84	302.0
85	213.0
86	124.0
87	90.5
88	57.0
89	42.0
90	27.0
91	22.0
92	17.0
93	15.5
94	14.0
95	16.0
96	18.0
97	16.5
98	15.0
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0074073124418632804
2	8.546898971380708E-4
3	9.971382133277494E-4
4	0.0
5	0.0
6	1.424483161896785E-4
7	1.424483161896785E-4
8	0.0
9	0.0014244831618967849
10	0.0018518281104658201
11	0.0
12	1.424483161896785E-4
13	8.546898971380708E-4
14	1.424483161896785E-4
15	0.0027065180076038912
16	7.122415809483924E-4
17	0.0
18	5.69793264758714E-4
19	0.0
20	9.971382133277494E-4
21	0.0
22	0.0
23	5.69793264758714E-4
24	5.69793264758714E-4
25	0.0017093797942761416
26	0.00555548433139746
27	0.006695070860914889
28	0.0037036562209316402
29	0.002279173059034856
30	0.003988552853310998
31	0.008262002339001352
32	0.003561207904741962
33	0.00655262254472521
34	0.0021367247428451773
35	0.0027065180076038912
36	0.00655262254472521
37	0.0031338629561729263
38	0.008689347287570388
39	0.003988552853310998
40	0.0024216213752245343
41	0.003846104537121319
42	0.002279173059034856
43	0.0017093797942761416
44	8.546898971380708E-4
45	7.122415809483924E-4
46	0.001139586529517428
47	0.0021367247428451773
48	0.001994276426655499
49	4.273449485690354E-4
50	9.971382133277494E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	702009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.839281824091415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23639555981423	48.380825139244465
2	13.693930434799007	16.936456468740772
3	4.091372748276131	7.590226572841654
4	1.5877403727390211	3.927388974931852
5	0.7600855886138534	2.350157346236125
6	0.4114783566925261	1.526731563841388
7	0.24779583691400217	1.0726461615633103
8	0.15881524272347128	0.7856816442190581
9	0.10844164984813681	0.6035358371785535
>10	0.5802088129594061	6.65117746539097
>50	0.06789248062834762	2.9523604477820857
>100	0.053294069204285616	6.2164876348983285
>500	0.00231713344329351	0.8598824980111519
>1k	2.3171334432935098E-4	0.1464422451203795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1022	0.14558217914585142	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	886	0.12620920814405515	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	713	0.10156564944324076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.424483161896785E-4
2	0.0	0.0	0.0	0.0	1.424483161896785E-4
3	0.0	0.0	0.0	0.0	1.424483161896785E-4
4	0.0	0.0	0.0	0.0	1.424483161896785E-4
5	0.0	0.0	0.0	0.0	1.424483161896785E-4
6	0.0	0.0	0.0	0.0	1.424483161896785E-4
7	0.0	0.0	0.0	0.0	1.424483161896785E-4
8	0.0	0.0	0.0	0.0	1.424483161896785E-4
9	0.0	0.0	0.0	0.0	1.424483161896785E-4
10	0.0	0.0	0.0	0.0	1.424483161896785E-4
11	0.0	0.0	0.0	0.0	2.84896632379357E-4
12	0.0	0.0	0.0	0.0	4.2734494856903546E-4
13	0.0	0.0	0.0	0.0	4.2734494856903546E-4
14	0.0	0.0	0.0	0.0	4.2734494856903546E-4
15	0.0	0.0	0.0	0.0	5.69793264758714E-4
16	0.0	0.0	0.0	1.424483161896785E-4	5.69793264758714E-4
17	0.0	0.0	0.0	2.84896632379357E-4	5.69793264758714E-4
18	0.0	0.0	0.0	2.84896632379357E-4	7.122415809483924E-4
19	0.0	0.0	0.0	2.84896632379357E-4	7.122415809483924E-4
20	0.0	0.0	0.0	4.2734494856903546E-4	8.546898971380709E-4
21	0.0	0.0	0.0	7.122415809483924E-4	8.546898971380709E-4
22	0.0	0.0	0.0	9.971382133277494E-4	8.546898971380709E-4
23	0.0	0.0	0.0	0.001994276426655499	8.546898971380709E-4
24	0.0	0.0	0.0	0.002279173059034856	8.546898971380709E-4
25	0.0	0.0	0.0	0.0027065180076038912	8.546898971380709E-4
26	0.0	0.0	0.0	0.0034187595885522837	0.001139586529517428
27	0.0	0.0	0.0	0.003988552853310998	0.001139586529517428
28	0.0	0.0	0.0	0.006125277596156175	0.001139586529517428
29	0.0	0.0	0.0	0.00840445065519103	0.001139586529517428
30	0.0	0.0	0.0	0.01609665972943367	0.001139586529517428
31	0.0	0.0	0.0	0.03219331945886734	0.001139586529517428
32	0.0	0.0	0.0	0.046295702761645505	0.001139586529517428
33	0.0	0.0	0.0	0.06182256922632046	0.001139586529517428
34	0.0	0.0	0.0	0.08034085033097867	0.001139586529517428
35	0.0	0.0	0.0	0.10028361459753365	0.001139586529517428
36	0.0	0.0	0.0	0.12692144972500352	0.001139586529517428
37	0.0	0.0	0.0	0.164812701831458	0.001139586529517428
38	0.0	0.0	0.0	0.2225042698882778	0.001139586529517428
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	30	0.00574594	29.330671	40
TATGTCG	40	7.029974E-4	27.499466	16
CCAAGCG	45	0.0013983686	24.44223	9
GACGTGA	265	0.0	24.073288	7
TAGGACG	360	0.0	23.220118	4
TTAGGAC	315	0.0	23.04553	3
CAAGCGC	50	0.0025807284	21.998007	10
CAATACT	80	3.6148122E-6	21.998005	4
ACGACGG	150	0.0	21.998005	6
CGTTTAT	145	1.2732926E-11	21.245508	31
GATTTCG	180	0.0	20.775894	41
ATTTCGT	185	0.0	20.214382	42
ACCCTAT	55	0.004484124	19.998186	1
AACGATT	55	0.004484124	19.998186	39
GTGTAGA	200	0.0	19.798206	1
AGGACGT	430	0.0	19.440098	5
GATATAC	215	0.0	19.440098	1
CGACGGT	170	5.456968E-12	19.410006	7
CGTGAAA	340	0.0	19.410006	9
TACCTTG	80	8.987989E-5	19.248255	7
>>END_MODULE