FastQCFastQC Report
Thu 2 Feb 2017
SRR4064092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865405
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13780.15923180476193227No Hit
TATCAACGCAGAGTACTTTTTTTTT9340.10792634662383509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC401.28879165E-518.9920233
GGTATCA2800.016.2798181
CAAACGC400.005275103714.2506089
CACCGAT400.005275103714.2506087
GCGCCGG400.005279153614.248966
CTAGGGT551.9626702E-413.8147754
ATAGCAC551.9655602E-413.812383
GTGTAGG1450.013.7536381
GTATCAA9600.013.5523821
CCAGTAC852.7081478E-713.4061343
TGGCGAG3050.012.77473118
AGAACCG604.09528E-412.6642755
AATACCT604.09528E-412.6642755
GGCGAGG2150.012.37691619
TTAATTC853.9561346E-612.2889563
GAACCGC701.0905648E-412.2133946
TAGACAG701.091743E-412.2119815
GTTCTAA550.003074883312.0865311
CTCCTAT802.8735465E-511.8707011
CAGTACT1052.7229908E-711.7590064
Produced by FastQC (version 0.11.5)