##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064092_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865405
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266226795546594	32.0	32.0	32.0	32.0	32.0
2	30.913989403805154	32.0	32.0	32.0	32.0	32.0
3	30.917290748262374	32.0	32.0	32.0	32.0	32.0
4	30.945475239916572	32.0	32.0	32.0	32.0	32.0
5	30.886187392030322	32.0	32.0	32.0	32.0	32.0
6	34.5318134283948	36.0	36.0	36.0	32.0	36.0
7	34.47550799914491	36.0	36.0	36.0	32.0	36.0
8	34.443614261530726	36.0	36.0	36.0	32.0	36.0
9	34.562081337639604	36.0	36.0	36.0	32.0	36.0
10	34.30034376968009	36.0	36.0	36.0	32.0	36.0
11	34.52669328233602	36.0	36.0	36.0	32.0	36.0
12	34.3827005852751	36.0	36.0	36.0	32.0	36.0
13	34.43502753046261	36.0	36.0	36.0	32.0	36.0
14	34.34905968881622	36.0	36.0	36.0	32.0	36.0
15	34.30902640960013	36.0	36.0	36.0	32.0	36.0
16	34.30158827369844	36.0	36.0	36.0	32.0	36.0
17	34.24878756189299	36.0	36.0	36.0	32.0	36.0
18	34.231419970996235	36.0	36.0	36.0	32.0	36.0
19	34.245174224784925	36.0	36.0	36.0	32.0	36.0
20	34.225121186034286	36.0	36.0	36.0	32.0	36.0
21	34.2160502885932	36.0	36.0	36.0	32.0	36.0
22	34.196187912018075	36.0	36.0	36.0	32.0	36.0
23	34.14545444040652	36.0	36.0	36.0	32.0	36.0
24	34.14097907915947	36.0	36.0	36.0	32.0	36.0
25	33.74570981216887	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	8.0
5	10.0
6	43.0
7	9.0
8	42.0
9	60.0
10	84.0
11	19.0
12	52.0
13	53.0
14	83.0
15	147.0
16	226.0
17	314.0
18	416.0
19	504.0
20	757.0
21	1092.0
22	1788.0
23	2891.0
24	4264.0
25	6165.0
26	9024.0
27	12268.0
28	16638.0
29	22179.0
30	29384.0
31	39781.0
32	55837.0
33	79382.0
34	173573.0
35	408310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19585625555595	17.63929332314525	12.043284708743757	26.12156571255504
2	16.132577276461895	19.536451008647198	38.30166854063074	26.02930317426017
3	18.836665927117423	23.38096492687707	28.87766468837244	28.904704457633073
4	12.845600419700986	16.039349278581152	36.02229314819651	35.092757153521354
5	14.45544485803534	36.708227351468416	34.096710931550255	14.739616858945986
6	33.82164294844088	35.51470129365837	17.197890206610904	13.465765551289843
7	30.045838688500336	30.58929182346904	20.77743155403094	18.587437933999684
8	27.348380198248705	34.21969193422535	19.297748750108372	19.134179117417567
9	27.19102110844176	14.716022206755905	18.874239561969198	39.21871712283314
10	15.958236497970793	27.619757882687573	32.23270549331113	24.189300126030503
11	35.943327945464596	21.55147495797231	23.117772335953273	19.38742476060982
12	24.30307998811342	23.939198072246807	29.35580066162451	22.401921278015266
13	29.637689622356795	20.319006118099793	24.974821088075892	25.06848317146752
14	22.926644819763045	19.895791657319677	25.780464057834934	31.39709946508234
15	24.694515618785093	28.00917146900756	22.2174558539225	25.078857058284846
16	24.63177734352416	26.49746192893401	24.431969288761955	24.438791438779877
17	23.923500300633645	26.36082974885528	25.50575829055085	24.209911659960223
18	23.84899158108983	26.30631880840041	26.370732722731056	23.4739568877787
19	25.179648127889575	25.471525742819278	25.651347332048946	23.697478797242198
20	25.617914638560556	25.30775751309957	25.374484083342296	23.699843764997578
21	25.65768628566539	25.02098814256673	24.96987652265216	24.351449049115722
22	25.460781796777244	25.24926971086795	25.230072878576564	24.059875613778235
23	24.399546653097097	25.61201831891567	25.526900124901697	24.461534903085536
24	24.40839436443809	25.489434188751776	25.741769329600345	24.36040211720979
25	24.48899111957637	25.548882805931694	25.53350194458033	24.42862412991161
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	25.0
2	83.0
3	141.0
4	141.0
5	141.0
6	340.0
7	539.0
8	539.0
9	539.0
10	802.0
11	1065.0
12	1065.0
13	1065.0
14	1642.0
15	2219.0
16	2219.0
17	2219.0
18	4116.5
19	6014.0
20	6014.0
21	6014.0
22	9650.5
23	13287.0
24	13287.0
25	13287.0
26	19556.0
27	25825.0
28	25825.0
29	25825.0
30	33696.5
31	41568.0
32	41568.0
33	41568.0
34	50288.0
35	59008.0
36	59008.0
37	59008.0
38	67747.0
39	76486.0
40	76486.0
41	76486.0
42	86646.5
43	96807.0
44	96807.0
45	96807.0
46	105942.0
47	115077.0
48	115077.0
49	115077.0
50	116169.0
51	117261.0
52	117261.0
53	117261.0
54	111102.5
55	104944.0
56	104944.0
57	104944.0
58	94316.5
59	83689.0
60	83689.0
61	83689.0
62	70329.0
63	56969.0
64	56969.0
65	56969.0
66	45680.5
67	34392.0
68	34392.0
69	34392.0
70	25421.5
71	16451.0
72	16451.0
73	16451.0
74	11965.5
75	7480.0
76	7480.0
77	7480.0
78	5488.5
79	3497.0
80	3497.0
81	3497.0
82	2439.0
83	1381.0
84	1381.0
85	1381.0
86	972.0
87	563.0
88	563.0
89	563.0
90	385.0
91	207.0
92	207.0
93	207.0
94	159.5
95	112.0
96	112.0
97	112.0
98	255.0
99	398.0
100	398.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039519069106372165
2	0.004391007678485796
3	0.0015021868373767196
4	0.003119926508397802
5	0.009706438026126496
6	0.012364153199946845
7	0.02276390822793952
8	0.038132435102639806
9	0.05118990530445283
10	0.06182076599973423
11	0.05766086398853716
12	0.06517179817542075
13	0.06863838318475164
14	0.06921614735297346
15	0.06343850567075532
16	0.06644287934550876
17	0.06528735100906513
18	0.07915369104638868
19	0.07614931737163524
20	0.0788070325454556
21	0.07291383802959307
22	0.0784603740445225
23	0.0837758043921632
24	0.0788070325454556
25	0.08019366654918796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	865405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.91470943981963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.27088371238807	25.865074621982913
2	17.122419333745277	14.696073012288572
3	8.054796048669736	10.370076960770069
4	4.611982337588199	7.916875278367108
5	2.7715298611860972	5.946969934829251
6	1.823877915426415	4.696271447653713
7	1.2531790906442628	3.764587158573915
8	0.8992063646927244	3.087134389377981
9	0.6397218556861648	2.470812980316659
>10	2.4557217096988215	16.645912370122947
>50	0.0644553214373604	1.8860357448859149
>100	0.030601585877019322	2.1157402549722226
>500	0.0013540524665790262	0.37828808078351006
>1k	2.7081049331580527E-4	0.16014776507516953
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1378	0.15923180476193227	No Hit
TATCAACGCAGAGTACTTTTTTTTT	934	0.10792634662383509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.1555283364436305E-4	0.0
6	0.0	0.0	0.0	1.1555283364436305E-4	0.0
7	0.0	0.0	0.0	2.311056672887261E-4	0.0
8	0.0	0.0	0.0	2.311056672887261E-4	0.0
9	0.0	0.0	0.0	3.4665850093308913E-4	0.0
10	0.0	0.0	0.0	3.4665850093308913E-4	0.0
11	0.0	0.0	0.0	5.777641682218152E-4	0.0
12	0.0	0.0	0.0	5.777641682218152E-4	0.0
13	0.0	0.0	0.0	5.777641682218152E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	40	1.28879165E-5	18.992023	3
GGTATCA	280	0.0	16.279818	1
CAAACGC	40	0.0052751037	14.250608	9
CACCGAT	40	0.0052751037	14.250608	7
GCGCCGG	40	0.0052791536	14.24896	6
CTAGGGT	55	1.9626702E-4	13.814775	4
ATAGCAC	55	1.9655602E-4	13.81238	3
GTGTAGG	145	0.0	13.753638	1
GTATCAA	960	0.0	13.552382	1
CCAGTAC	85	2.7081478E-7	13.406134	3
TGGCGAG	305	0.0	12.774731	18
AGAACCG	60	4.09528E-4	12.664275	5
AATACCT	60	4.09528E-4	12.664275	5
GGCGAGG	215	0.0	12.376916	19
TTAATTC	85	3.9561346E-6	12.288956	3
GAACCGC	70	1.0905648E-4	12.213394	6
TAGACAG	70	1.091743E-4	12.211981	5
GTTCTAA	55	0.0030748833	12.086531	1
CTCCTAT	80	2.8735465E-5	11.870701	1
CAGTACT	105	2.7229908E-7	11.759006	4
>>END_MODULE