FastQCFastQC Report
Thu 2 Feb 2017
SRR4064092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865405
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.031.1008931
GGTATCA3300.030.6713371
GTATAAC609.737276E-625.670581
TATCAAC15200.020.8404622
GCGTTAT654.931304E-420.30726443
CGGTTCG550.004483145719.9995841
AGTACCG550.00448464519.9984255
ATCAACG15750.019.973033
TCAACGC15850.019.8470154
CTTAGTA1001.1235297E-619.798442
CAACGCA16500.019.331815
TTAGGAC3450.019.1289273
AGTCGAC600.007408869518.33400726
AACGCAG17700.018.1454646
TGGACCG851.4311016E-418.1162195
TAGGACG4300.017.9055654
ATTAGTC750.0012911517.5986143
CTATTAG902.2118796E-417.1137181
GACGTGA3050.016.5888567
CATTATA1351.0854637E-616.2950122
Produced by FastQC (version 0.11.5)