##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865405
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38908603486229	32.0	32.0	32.0	32.0	32.0
2	31.45531051935221	32.0	32.0	32.0	32.0	32.0
3	31.49638955171278	32.0	32.0	32.0	32.0	32.0
4	31.568896643767946	32.0	32.0	32.0	32.0	32.0
5	31.537906529312863	32.0	32.0	32.0	32.0	32.0
6	35.11409571241211	36.0	36.0	36.0	36.0	36.0
7	35.126375512043495	36.0	36.0	36.0	36.0	36.0
8	35.08072520958395	36.0	36.0	36.0	36.0	36.0
9	35.175753548916404	36.0	36.0	36.0	36.0	36.0
10	35.04889387049994	36.0	36.0	36.0	36.0	36.0
11	35.17418780802052	36.0	36.0	36.0	36.0	36.0
12	35.09095741300316	36.0	36.0	36.0	36.0	36.0
13	35.129868674204566	36.0	36.0	36.0	36.0	36.0
14	35.06650412234734	36.0	36.0	36.0	36.0	36.0
15	35.05504474783483	36.0	36.0	36.0	36.0	36.0
16	35.068224704040304	36.0	36.0	36.0	36.0	36.0
17	35.03599932979356	36.0	36.0	36.0	36.0	36.0
18	35.034131996001875	36.0	36.0	36.0	36.0	36.0
19	35.03231203887197	36.0	36.0	36.0	36.0	36.0
20	35.028481462436666	36.0	36.0	36.0	36.0	36.0
21	35.02716300460478	36.0	36.0	36.0	36.0	36.0
22	35.002450875601596	36.0	36.0	36.0	36.0	36.0
23	34.946845696523596	36.0	36.0	36.0	36.0	36.0
24	34.94162617502788	36.0	36.0	36.0	36.0	36.0
25	34.91808228517284	36.0	36.0	36.0	36.0	36.0
26	34.85650071353875	36.0	36.0	36.0	32.0	36.0
27	34.83841091743172	36.0	36.0	36.0	32.0	36.0
28	34.81809441822037	36.0	36.0	36.0	32.0	36.0
29	34.80685690514846	36.0	36.0	36.0	32.0	36.0
30	34.798084134018175	36.0	36.0	36.0	32.0	36.0
31	34.77902947175022	36.0	36.0	36.0	32.0	36.0
32	34.74390141032234	36.0	36.0	36.0	32.0	36.0
33	34.73773666664741	36.0	36.0	36.0	32.0	36.0
34	34.73916605519959	36.0	36.0	36.0	32.0	36.0
35	34.70194995406775	36.0	36.0	36.0	32.0	36.0
36	34.67239847239154	36.0	36.0	36.0	32.0	36.0
37	34.65812191979478	36.0	36.0	36.0	32.0	36.0
38	34.63485997885383	36.0	36.0	36.0	32.0	36.0
39	34.61463245532438	36.0	36.0	36.0	32.0	36.0
40	34.6129268954998	36.0	36.0	36.0	32.0	36.0
41	34.59513060359023	36.0	36.0	36.0	32.0	36.0
42	34.5676648505613	36.0	36.0	36.0	32.0	36.0
43	34.55189651088219	36.0	36.0	36.0	32.0	36.0
44	34.50740404781576	36.0	36.0	36.0	32.0	36.0
45	34.48433623563534	36.0	36.0	36.0	32.0	36.0
46	34.448386593560244	36.0	36.0	36.0	32.0	36.0
47	34.42597743253159	36.0	36.0	36.0	32.0	36.0
48	34.375463511303955	36.0	36.0	36.0	32.0	36.0
49	34.373871193256335	36.0	36.0	36.0	32.0	36.0
50	33.8048416637297	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	6.0
21	20.0
22	59.0
23	224.0
24	569.0
25	1465.0
26	3002.0
27	5496.0
28	9220.0
29	14818.0
30	21024.0
31	29917.0
32	42710.0
33	67511.0
34	138931.0
35	530429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33068926146265	18.570329076007823	12.586267467284232	26.51271419524529
2	15.410890442696507	20.294175763605544	37.82396831064443	26.470965483053522
3	17.9925422028812	23.9900947882085	29.197264164787967	28.820098844122334
4	12.542566775093741	16.678087138391852	36.048786406364655	34.73055968014976
5	14.060699903513385	37.162600169862664	33.9701064819362	14.806593444687746
6	33.833716777039136	35.87369121669327	17.037611378744934	13.25498062752267
7	30.05313125430435	30.6989567878124	20.78751658185079	18.460395376032466
8	27.43050941466712	34.336755623089765	19.142829080026114	19.089905882216996
9	27.2529674413445	14.675083661508312	18.71787582968181	39.35407306746538
10	15.96021271039355	27.686323812517188	32.35814107201047	23.995322405078795
11	36.14296196578481	21.503920129881386	23.104904640024035	19.248213264309776
12	24.370177096886763	24.080600691932766	29.371205520671317	22.17801669050915
13	29.846694992714344	20.281165104188933	25.092125135342197	24.780014767754526
14	23.03657722075983	19.846152423960195	25.715910062606746	31.401360292673232
15	24.744907981982355	28.04405956268619	22.24876180703372	24.96227064829773
16	24.691529215459244	26.516469878599214	24.404378101174256	24.387622804767286
17	23.90938346785609	26.38706732685852	25.650533565209354	24.053015640076033
18	23.968574215048694	26.198168716576305	26.432279028060883	23.40097804031412
19	25.252800711805456	25.419543450754272	25.77117072353407	23.556485113906206
20	25.83987270611591	25.214496434592597	25.356858512662946	23.588772346628552
21	25.863497437615912	24.97142956188143	24.963340863526327	24.20173213697633
22	25.59452001608497	25.234225864451748	25.29396674847817	23.877287370985112
23	24.560493273646237	25.465043991116264	25.561877601392187	24.41258513384531
24	24.53440659301295	25.520770140085347	25.653194299521264	24.29162896738044
25	24.657611810601438	25.424431933736386	25.481515891064394	24.43644036459778
26	24.366709384869438	25.771441051769145	25.74752039900111	24.114329164360306
27	24.73762061593575	25.771190847633907	25.08175882590859	24.409429710521753
28	24.5568947386667	25.49487502455597	25.41768260975074	24.53054762702659
29	24.5153007573534	25.62960634725474	25.37087667642729	24.484216218964573
30	24.543256841613836	25.535207068454273	25.593101647164325	24.32843444276757
31	24.953313148588993	25.243603670233668	25.232278641921095	24.570804539256248
32	24.86407595337502	25.624903943155147	25.15250649142046	24.35851361204937
33	24.332076064749334	25.771925932089008	25.466044503610025	24.429953499551633
34	24.71939975294005	25.49998439986272	25.673550371687714	24.10706547550952
35	24.760711780618667	25.648415318606066	25.65165089515692	23.939222005618348
36	24.330898837451176	25.71867705179467	25.620334203896732	24.33008990685742
37	24.897933713826127	25.24275771376141	25.162791826376957	24.696516746035506
38	24.509958004654848	25.589078899014716	25.475596706011782	24.42536639031865
39	24.934883043706364	25.22273812467788	25.385212730798294	24.457166100817457
40	25.037209087337352	25.073378169128013	25.640296748249327	24.249115995285308
41	24.69932284083293	25.210312232776356	25.634172271141004	24.456192655249716
42	24.73500478973137	25.4363046615815	25.710630892554022	24.11805965613311
43	24.508025283398236	25.208056483204107	25.794612833520148	24.489305399877512
44	24.316728988809746	25.389185991153184	25.45308737271723	24.84099764731984
45	24.589260333491257	25.24514958573591	25.452917181848644	24.712672898924186
46	24.47402631168426	24.99298008423899	25.59432397922336	24.93866962485339
47	24.67918903147154	25.030939986248896	25.720228568787302	24.56964241349226
48	24.164193798879584	25.317197293249766	25.63462569563851	24.883983212232145
49	24.511210948874744	25.21446630706583	25.62895480030136	24.64536794375806
50	24.354340335153697	25.671081611381634	25.561073915465094	24.41350413799957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	7.5
2	12.0
3	28.0
4	44.0
5	70.5
6	97.0
7	99.0
8	101.0
9	129.0
10	157.0
11	255.5
12	354.0
13	625.0
14	896.0
15	1175.5
16	1455.0
17	1495.5
18	1536.0
19	1550.0
20	1564.0
21	1770.0
22	1976.0
23	2477.5
24	2979.0
25	3992.0
26	5005.0
27	6182.0
28	7359.0
29	9442.5
30	11526.0
31	14045.0
32	16564.0
33	19641.0
34	22718.0
35	27245.5
36	31773.0
37	34476.5
38	37180.0
39	39627.5
40	42075.0
41	45211.5
42	48348.0
43	49822.5
44	51297.0
45	54322.5
46	57348.0
47	59895.0
48	62442.0
49	65076.0
50	67710.0
51	68500.5
52	69291.0
53	67808.0
54	66325.0
55	64906.5
56	63488.0
57	59006.5
58	54525.0
59	48567.0
60	42609.0
61	36817.0
62	31025.0
63	27030.0
64	23035.0
65	19163.0
66	15291.0
67	13123.0
68	10955.0
69	8761.5
70	6568.0
71	5098.5
72	3629.0
73	2953.0
74	2277.0
75	1863.5
76	1450.0
77	1231.0
78	1012.0
79	785.0
80	558.0
81	476.5
82	395.0
83	308.5
84	222.0
85	174.0
86	126.0
87	86.5
88	47.0
89	36.0
90	25.0
91	20.0
92	15.0
93	10.5
94	6.0
95	6.5
96	7.0
97	4.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008435356856038502
2	4.6221133457745214E-4
3	2.3110566728872607E-4
4	0.0
5	0.0
6	2.3110566728872607E-4
7	1.1555283364436303E-4
8	0.0
9	0.0015021868373767196
10	3.4665850093308913E-4
11	0.0
12	3.4665850093308913E-4
13	6.933170018661783E-4
14	3.4665850093308913E-4
15	0.0035821378429752543
16	8.088698355105412E-4
17	0.0
18	1.1555283364436303E-4
19	0.0
20	6.933170018661783E-4
21	0.0
22	1.1555283364436303E-4
23	3.4665850093308913E-4
24	4.6221133457745214E-4
25	0.0015021868373767196
26	0.005084324680351974
27	0.006355405850439967
28	0.004044349177552706
29	0.00265771517382035
30	0.004506560512130158
31	0.0075109341868835975
32	0.003697690676619617
33	0.004275454844841433
34	0.003235479342042165
35	0.003004373674753439
36	0.0075109341868835975
37	0.004159902011197069
38	0.008204251188749775
39	0.004506560512130158
40	0.0028888208411090763
41	0.0028888208411090763
42	0.0013866340037323565
43	0.0017332925046654455
44	0.0010399755027992673
45	0.0017332925046654455
46	0.0011555283364436304
47	0.002311056672887261
48	0.0015021868373767196
49	1.1555283364436303E-4
50	0.0012710811700879935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	865405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.49691852596288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.17209412428528	28.28638700961103
2	16.342594422332613	14.870753738714445
3	7.80943722795247	10.65915987881325
4	4.375004772591191	7.961969427571206
5	2.645826564010459	6.018847780830608
6	1.773979087372067	4.842634920295735
7	1.2063974056846578	3.842115513345749
8	0.8024917263778457	2.9208720554217713
9	0.6197827598082155	2.5378385154111722
>10	2.1879757698261924	15.405057052999046
>50	0.04907963874752885	1.522824241373824
>100	0.01533650101154804	1.1315398656121636
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.1555283364436305E-4	0.0
6	0.0	0.0	0.0	1.1555283364436305E-4	0.0
7	0.0	0.0	0.0	1.1555283364436305E-4	0.0
8	0.0	0.0	0.0	1.1555283364436305E-4	0.0
9	0.0	0.0	0.0	2.311056672887261E-4	0.0
10	0.0	0.0	0.0	2.311056672887261E-4	0.0
11	0.0	0.0	0.0	4.622113345774522E-4	0.0
12	0.0	0.0	0.0	4.622113345774522E-4	0.0
13	0.0	0.0	0.0	4.622113345774522E-4	0.0
14	0.0	0.0	0.0	4.622113345774522E-4	0.0
15	0.0	0.0	0.0	4.622113345774522E-4	0.0
16	0.0	0.0	0.0	4.622113345774522E-4	0.0
17	0.0	0.0	0.0	4.622113345774522E-4	0.0
18	0.0	0.0	0.0	4.622113345774522E-4	0.0
19	0.0	0.0	0.0	4.622113345774522E-4	0.0
20	0.0	0.0	0.0	5.777641682218152E-4	0.0
21	0.0	0.0	0.0	5.777641682218152E-4	0.0
22	0.0	0.0	0.0	5.777641682218152E-4	0.0
23	0.0	0.0	0.0	5.777641682218152E-4	0.0
24	0.0	0.0	0.0	5.777641682218152E-4	0.0
25	0.0	0.0	0.0	6.933170018661783E-4	0.0
26	0.0	0.0	0.0	9.244226691549044E-4	0.0
27	0.0	0.0	0.0	0.0011555283364436304	0.0
28	0.0	0.0	0.0	0.002195503839242898	0.0
29	0.0	0.0	0.0	0.004853219013063248	0.0
30	0.0	0.0	0.0	0.010399755027992674	0.0
31	0.0	0.0	0.0	0.028310444242868944	0.0
32	0.0	0.0	0.0	0.046105580624100855	0.0
33	0.0	0.0	0.0	0.06678953784644184	0.0
34	0.0	0.0	0.0	0.08932234040709264	0.0
35	0.0	0.0	0.0	0.11624615064622922	0.0
36	0.0	0.0	0.0	0.15518745558437957	0.0
37	0.0	0.0	0.0	0.2188570669224236	0.0
38	0.0	0.0	0.0	0.31857916235750894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1040	0.0	31.100893	1
GGTATCA	330	0.0	30.671337	1
GTATAAC	60	9.737276E-6	25.67058	1
TATCAAC	1520	0.0	20.840462	2
GCGTTAT	65	4.931304E-4	20.307264	43
CGGTTCG	55	0.0044831457	19.99958	41
AGTACCG	55	0.004484645	19.998425	5
ATCAACG	1575	0.0	19.97303	3
TCAACGC	1585	0.0	19.847015	4
CTTAGTA	100	1.1235297E-6	19.79844	2
CAACGCA	1650	0.0	19.33181	5
TTAGGAC	345	0.0	19.128927	3
AGTCGAC	60	0.0074088695	18.334007	26
AACGCAG	1770	0.0	18.145464	6
TGGACCG	85	1.4311016E-4	18.116219	5
TAGGACG	430	0.0	17.905565	4
ATTAGTC	75	0.00129115	17.598614	3
CTATTAG	90	2.2118796E-4	17.113718	1
GACGTGA	305	0.0	16.588856	7
CATTATA	135	1.0854637E-6	16.295012	2
>>END_MODULE