FastQCFastQC Report
Thu 2 Feb 2017
SRR4064091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333881
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106303.1837690674222254No Hit
TATCAACGCAGAGTACTTTTTTTTT80042.3972612996846183No Hit
GTACTTTTTTTTTTTTTTTTTTTTT74422.2289378551040637No Hit
GTACATGGGGTGGTATCAACGCAAA64441.9300289624147526No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50231.504428224427266No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43761.3106466076236742No Hit
GTATCAACGCAGAGTACATGGGGTG37101.1111743405584624No Hit
GGTATCAACGCAGAGTACTTTTTTT36451.0917063265055513No Hit
GTACATGGGTGGTATCAACGCAAAA28110.8419167308112772No Hit
TATCAACGCAGAGTACATGGGGTGG26080.7811166253844932No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21980.6583183828969004No Hit
GTACATGGGAGTGGTATCAACGCAA20280.6074020384508253No Hit
GGTATCAACGCAGAGTACATGGGGT18440.5522925832856617No Hit
ACGCAGAGTACATGGGGTGGTATCA16050.4807101931526502No Hit
ATCAACGCAGAGTACTTTTTTTTTT13880.41571697700677784No Hit
GTATCAACGCAGAGTACATGGGAGT11870.3555158873970067No Hit
GTATCAACGCAGAGTACATGGGTGG10050.3010054480488557No Hit
GAGTACATGGGGTGGTATCAACGCA8790.2632674515770589No Hit
TATCAACGCAGAGTACATGGGAGTG7930.237509771445515No Hit
TATCAACGCAGAGTACATGGGTGGT7580.2270269946477937No Hit
GGTGGTATCAACGCAAAAAAAAAAA7260.21744274157559132No Hit
GTACATGGGCAGTGGTATCAACGCA7020.2102545517714395No Hit
ATCAACGCAGAGTACATGGGGTGGT7000.20965553595442687No Hit
GTATCAACGCAGAGTACATGGGAAG7000.20965553595442687No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT6800.20366537778430038No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT6680.20007128288222453No Hit
GTATTAGAGGCACTGCCTGCCCAGT6610.19797472752268022No Hit
CCATAGGGTCTTCTCGTCTTATTAT6520.1952791563461233No Hit
GGTATCAACGCAGAGTACATGGGAG6480.19408112471209804No Hit
GTATCAACGCAGAGTACATGGGCAG6370.19078653771852846No Hit
GTACATGGGGTATCAACGCAAAAAA6300.1886899823589842No Hit
CAACGCAGAGTACTTTTTTTTTTTT5820.1743136027506806No Hit
CCATTGGGATGTCCTGATCCAACAT5770.17281606320814902No Hit
ACGCAGAGTACATGGGTGGTATCAA5400.16173427059341502No Hit
GGTATCAACGCAGAGTACATGGGTG5140.15394706497225058No Hit
GTATCAACGCAGAGTACATGGGGTA4850.14526133562556717No Hit
AACGCAGAGTACTTTTTTTTTTTTT4510.13507806673635217No Hit
TCAACGCAGAGTACTTTTTTTTTTT4370.13088495601726363No Hit
GATATATTTTGATCAACGGACCAAG4330.12968692438323834No Hit
ACGCAGAGTACATGGGAGTGGTATC4320.129387416474732No Hit
CTTCTACACCATTGGGATGTCCTGA4310.1290879085662257No Hit
CTCTAATACTTGTAATGCTAGAGGT4270.12788987693220039No Hit
GTATCAACGCAGAGTACATGGGAGA4150.12429578203012452No Hit
TATCAACGCAGAGTACATGGGAAGC4060.12160021085356759No Hit
GAGTGGTATCAACGCAAAAAAAAAA4020.1204021792195423No Hit
GGTATCAACGCAGAGTACATGGGAA3940.1180061159514917No Hit
GTACATGGGAGAAATCGTAAATAGA3940.1180061159514917No Hit
GTCAGGATACCGCGGCCGTTAAACT3920.11740710013447904No Hit
ATCGTAAATAGATAGAAACCGACCT3900.11680808431746639No Hit
GTTATATAATTTAAGCTCCATAGGG3660.10961989451331462No Hit
GGGTGGTATCAACGCAAAAAAAAAA3560.10662481542825138No Hit
GTACATGGGGGTATCAACGCAAAAA3420.10243170470916284No Hit
TCCCAATGGTGTAGAAGCTATTAAT3380.10123367307513755No Hit
GTATCAACGCAGAGTACATGGGGGT3360.10063465725812491No Hit
GTATCCTGACCGTGCAAAGGTAGCA3350.10033514934961857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA307.694328E-419.0040518
ATCCAAA250.00602017319.001214
AGGCAAT607.63921E-817.4151557
TCGTCAC350.002160152716.29162619
GGCAAAC350.002162318216.28918515
GGTTTAC350.002166654716.2843028
TACATTA350.002168825616.281862
CTATAGG807.650669E-915.436163
GACAATT508.6655215E-515.203238518
GGCAATA704.410049E-714.9272768
CAATAAT704.410049E-714.92727610
CTATGGT456.747094E-414.7764954
ATATATC456.747094E-414.7764953
AACCTTG456.747094E-414.7764956
TAAGAGG653.3560027E-614.6141174
TTGCATA400.00525493714.25517319
TTTCGTC400.005260168614.25303617
TTTTTCG400.005260168614.25303615
ATTTTTC400.00526540514.25089914
AGGGGGC400.00526540514.25089914
Produced by FastQC (version 0.11.5)