##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064091_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333881
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.748041967048138	32.0	32.0	32.0	32.0	32.0
2	30.674380992030095	32.0	32.0	32.0	32.0	32.0
3	30.819166110081138	32.0	32.0	32.0	32.0	32.0
4	30.6887573716384	32.0	32.0	32.0	32.0	32.0
5	30.79403440147837	32.0	32.0	32.0	32.0	32.0
6	34.27550833979771	36.0	36.0	36.0	32.0	36.0
7	34.01023718031275	36.0	36.0	36.0	32.0	36.0
8	33.81623991781503	36.0	36.0	36.0	32.0	36.0
9	33.79927578987723	36.0	36.0	36.0	32.0	36.0
10	33.81644957335099	36.0	36.0	36.0	32.0	36.0
11	34.198343122250144	36.0	36.0	36.0	32.0	36.0
12	34.00543007838122	36.0	36.0	36.0	32.0	36.0
13	34.080244158847016	36.0	36.0	36.0	32.0	36.0
14	34.00504970333742	36.0	36.0	36.0	32.0	36.0
15	34.07139969030882	36.0	36.0	36.0	32.0	36.0
16	34.01156699542651	36.0	36.0	36.0	32.0	36.0
17	33.90209086470928	36.0	36.0	36.0	32.0	36.0
18	33.93212252269521	36.0	36.0	36.0	32.0	36.0
19	33.827534361044805	36.0	36.0	36.0	32.0	36.0
20	33.6633710813134	36.0	36.0	36.0	27.0	36.0
21	33.619523123508074	36.0	36.0	36.0	27.0	36.0
22	33.71633006969549	36.0	36.0	36.0	27.0	36.0
23	33.842950033095626	36.0	36.0	36.0	32.0	36.0
24	33.85454997439207	36.0	36.0	36.0	32.0	36.0
25	33.57777471614138	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	7.0
6	18.0
7	6.0
8	17.0
9	20.0
10	35.0
11	13.0
12	24.0
13	11.0
14	135.0
15	169.0
16	237.0
17	274.0
18	374.0
19	493.0
20	755.0
21	997.0
22	1484.0
23	2038.0
24	2662.0
25	3557.0
26	4716.0
27	5722.0
28	7596.0
29	9726.0
30	12366.0
31	16480.0
32	22616.0
33	31643.0
34	66904.0
35	142782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.403013160102596	18.79599443871803	13.02107054677949	17.779921854399884
2	14.407063954184936	22.72631969903913	42.05694534301392	20.809671003762013
3	17.63588877307743	34.473876529010774	28.938587978770563	18.951646719141237
4	9.403089235598392	16.123090194058147	36.98037254132609	37.493448029017365
5	10.22240860279471	43.64780062603903	29.943536671209692	16.186254099956567
6	24.941437258484857	39.439834646376895	21.13770481981847	14.481023275319775
7	26.125340762709325	35.94499865192775	21.02992720409814	16.89973338126479
8	31.681622747224758	27.468052913064973	19.591916225974142	21.258408113736124
9	31.626451868100254	12.216968127824325	22.08667457777458	34.06990542630084
10	18.394294019809102	27.274525375728608	29.098101502914425	25.23307910154787
11	31.513048273216576	25.570599729085004	24.617002912935593	18.299349084762827
12	27.625241641815645	28.879231541562394	28.340950982302076	15.15457583431988
13	28.690588228241907	22.774437577552256	29.84600444782794	18.688969746377897
14	24.7052264975453	21.60163766387129	32.40800129478549	21.285134543797916
15	22.47223337748821	27.522372135677337	32.07771657366172	17.927677913172737
16	19.24460216033472	29.509488928584272	29.132449378394256	22.113459532686754
17	16.70552834904784	27.22695422313599	29.98663317968483	26.08088424813134
18	17.435837484937323	27.142797191897028	35.58629041444099	19.83507490872466
19	20.813687551893338	25.328445492885553	36.211284197148224	17.646582758072878
20	24.18562405762607	22.529743794196026	33.927259973800886	19.357372174377023
21	27.376192217539614	21.50068041076428	32.817380148791145	18.305747222904962
22	23.29079621452617	23.79080970409873	33.18254988893585	19.735844192439245
23	21.068109598896818	25.845074644762878	34.96732418010672	18.11949157623359
24	19.948563926406848	27.63609877164901	34.570976386166215	17.844360915777926
25	20.26559151066413	27.757910040618118	34.586249793911776	17.390248654805976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	494.0
1	494.0
2	1162.0
3	1830.0
4	1830.0
5	1830.0
6	5222.5
7	8615.0
8	8615.0
9	8615.0
10	7562.5
11	6510.0
12	6510.0
13	6510.0
14	4291.5
15	2073.0
16	2073.0
17	2073.0
18	3779.5
19	5486.0
20	5486.0
21	5486.0
22	8451.0
23	11416.0
24	11416.0
25	11416.0
26	17136.0
27	22856.0
28	22856.0
29	22856.0
30	24790.5
31	26725.0
32	26725.0
33	26725.0
34	26099.0
35	25473.0
36	25473.0
37	25473.0
38	25087.5
39	24702.0
40	24702.0
41	24702.0
42	27106.5
43	29511.0
44	29511.0
45	29511.0
46	39288.5
47	49066.0
48	49066.0
49	49066.0
50	49074.5
51	49083.0
52	49083.0
53	49083.0
54	36371.5
55	23660.0
56	23660.0
57	23660.0
58	19731.0
59	15802.0
60	15802.0
61	15802.0
62	14074.0
63	12346.0
64	12346.0
65	12346.0
66	10373.5
67	8401.0
68	8401.0
69	8401.0
70	6455.0
71	4509.0
72	4509.0
73	4509.0
74	3575.0
75	2641.0
76	2641.0
77	2641.0
78	2056.5
79	1472.0
80	1472.0
81	1472.0
82	972.5
83	473.0
84	473.0
85	473.0
86	368.0
87	263.0
88	263.0
89	263.0
90	189.5
91	116.0
92	116.0
93	116.0
94	86.0
95	56.0
96	56.0
97	56.0
98	179.0
99	302.0
100	302.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043428646733416995
2	0.00509163444460751
3	0.0014975395425316205
4	0.0035940949020758896
5	0.010782284706227668
6	0.015274903333822528
7	0.021265061503949013
8	0.03773799647179684
9	0.05181486817159407
10	0.05870355006723952
11	0.05900305797574585
12	0.06768878732242925
13	0.07038435849898617
14	0.07038435849898617
15	0.06199813706080909
16	0.06858731104794823
17	0.06559223196288498
18	0.08236467483923912
19	0.07907008784566956
20	0.08446123019878339
21	0.07757254830313795
22	0.08685729346683399
23	0.09015188046040355
24	0.07877057993716324
25	0.0856592618328087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	333881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.559405896112686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.56706273875648	17.014145758518755
2	14.795284632871638	7.563173705601696
3	6.5058942089104494	4.988603724081334
4	3.2869296210363497	3.3604787334409565
5	1.915910848625466	2.4484771520391995
6	1.2362605170029763	1.8958850608450317
7	0.8507347254447023	1.522099191029139
8	0.5929363237947924	1.2124080136336
9	0.4933323958846001	1.134835465330462
>10	3.078347277883241	15.941308430249101
>50	0.3656050059762357	6.618226254264243
>100	0.27068832173240526	12.940239187015735
>500	0.02109259649862898	3.6081717737756867
>1k	0.014061730999085989	8.507522141122138
>5k	0.004687243666361996	8.0606563416307
>10k+	0.001171810916590499	3.1837690674222254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10630	3.1837690674222254	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8004	2.3972612996846183	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7442	2.2289378551040637	No Hit
GTACATGGGGTGGTATCAACGCAAA	6444	1.9300289624147526	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5023	1.504428224427266	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4376	1.3106466076236742	No Hit
GTATCAACGCAGAGTACATGGGGTG	3710	1.1111743405584624	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3645	1.0917063265055513	No Hit
GTACATGGGTGGTATCAACGCAAAA	2811	0.8419167308112772	No Hit
TATCAACGCAGAGTACATGGGGTGG	2608	0.7811166253844932	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2198	0.6583183828969004	No Hit
GTACATGGGAGTGGTATCAACGCAA	2028	0.6074020384508253	No Hit
GGTATCAACGCAGAGTACATGGGGT	1844	0.5522925832856617	No Hit
ACGCAGAGTACATGGGGTGGTATCA	1605	0.4807101931526502	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1388	0.41571697700677784	No Hit
GTATCAACGCAGAGTACATGGGAGT	1187	0.3555158873970067	No Hit
GTATCAACGCAGAGTACATGGGTGG	1005	0.3010054480488557	No Hit
GAGTACATGGGGTGGTATCAACGCA	879	0.2632674515770589	No Hit
TATCAACGCAGAGTACATGGGAGTG	793	0.237509771445515	No Hit
TATCAACGCAGAGTACATGGGTGGT	758	0.2270269946477937	No Hit
GGTGGTATCAACGCAAAAAAAAAAA	726	0.21744274157559132	No Hit
GTACATGGGCAGTGGTATCAACGCA	702	0.2102545517714395	No Hit
ATCAACGCAGAGTACATGGGGTGGT	700	0.20965553595442687	No Hit
GTATCAACGCAGAGTACATGGGAAG	700	0.20965553595442687	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	680	0.20366537778430038	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	668	0.20007128288222453	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	661	0.19797472752268022	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	652	0.1952791563461233	No Hit
GGTATCAACGCAGAGTACATGGGAG	648	0.19408112471209804	No Hit
GTATCAACGCAGAGTACATGGGCAG	637	0.19078653771852846	No Hit
GTACATGGGGTATCAACGCAAAAAA	630	0.1886899823589842	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	582	0.1743136027506806	No Hit
CCATTGGGATGTCCTGATCCAACAT	577	0.17281606320814902	No Hit
ACGCAGAGTACATGGGTGGTATCAA	540	0.16173427059341502	No Hit
GGTATCAACGCAGAGTACATGGGTG	514	0.15394706497225058	No Hit
GTATCAACGCAGAGTACATGGGGTA	485	0.14526133562556717	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	451	0.13507806673635217	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	437	0.13088495601726363	No Hit
GATATATTTTGATCAACGGACCAAG	433	0.12968692438323834	No Hit
ACGCAGAGTACATGGGAGTGGTATC	432	0.129387416474732	No Hit
CTTCTACACCATTGGGATGTCCTGA	431	0.1290879085662257	No Hit
CTCTAATACTTGTAATGCTAGAGGT	427	0.12788987693220039	No Hit
GTATCAACGCAGAGTACATGGGAGA	415	0.12429578203012452	No Hit
TATCAACGCAGAGTACATGGGAAGC	406	0.12160021085356759	No Hit
GAGTGGTATCAACGCAAAAAAAAAA	402	0.1204021792195423	No Hit
GGTATCAACGCAGAGTACATGGGAA	394	0.1180061159514917	No Hit
GTACATGGGAGAAATCGTAAATAGA	394	0.1180061159514917	No Hit
GTCAGGATACCGCGGCCGTTAAACT	392	0.11740710013447904	No Hit
ATCGTAAATAGATAGAAACCGACCT	390	0.11680808431746639	No Hit
GTTATATAATTTAAGCTCCATAGGG	366	0.10961989451331462	No Hit
GGGTGGTATCAACGCAAAAAAAAAA	356	0.10662481542825138	No Hit
GTACATGGGGGTATCAACGCAAAAA	342	0.10243170470916284	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	338	0.10123367307513755	No Hit
GTATCAACGCAGAGTACATGGGGGT	336	0.10063465725812491	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	335	0.10033514934961857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.995079085063241E-4	0.0
4	0.0	0.0	0.0	2.995079085063241E-4	0.0
5	0.0	0.0	0.0	2.995079085063241E-4	0.0
6	0.0	0.0	0.0	2.995079085063241E-4	0.0
7	0.0	0.0	0.0	2.995079085063241E-4	0.0
8	0.0	0.0	0.0	2.995079085063241E-4	0.0
9	0.0	0.0	0.0	2.995079085063241E-4	0.0
10	0.0	0.0	0.0	2.995079085063241E-4	0.0
11	0.0	0.0	0.0	2.995079085063241E-4	0.0
12	0.0	0.0	0.0	2.995079085063241E-4	0.0
13	0.0	0.0	0.0	5.990158170126482E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTCA	30	7.694328E-4	19.00405	18
ATCCAAA	25	0.006020173	19.0012	14
AGGCAAT	60	7.63921E-8	17.415155	7
TCGTCAC	35	0.0021601527	16.291626	19
GGCAAAC	35	0.0021623182	16.289185	15
GGTTTAC	35	0.0021666547	16.284302	8
TACATTA	35	0.0021688256	16.28186	2
CTATAGG	80	7.650669E-9	15.43616	3
GACAATT	50	8.6655215E-5	15.2032385	18
GGCAATA	70	4.410049E-7	14.927276	8
CAATAAT	70	4.410049E-7	14.927276	10
CTATGGT	45	6.747094E-4	14.776495	4
ATATATC	45	6.747094E-4	14.776495	3
AACCTTG	45	6.747094E-4	14.776495	6
TAAGAGG	65	3.3560027E-6	14.614117	4
TTGCATA	40	0.005254937	14.255173	19
TTTCGTC	40	0.0052601686	14.253036	17
TTTTTCG	40	0.0052601686	14.253036	15
ATTTTTC	40	0.005265405	14.250899	14
AGGGGGC	40	0.005265405	14.250899	14
>>END_MODULE