##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333881
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.20290762277578	32.0	32.0	32.0	21.0	32.0
2	31.316681691980076	32.0	32.0	32.0	32.0	32.0
3	31.438153114432986	32.0	32.0	32.0	32.0	32.0
4	31.576178338989042	32.0	32.0	32.0	32.0	32.0
5	31.385820097579675	32.0	32.0	32.0	32.0	32.0
6	34.863238698817845	36.0	36.0	36.0	36.0	36.0
7	34.96927947382451	36.0	36.0	36.0	36.0	36.0
8	35.01312443655075	36.0	36.0	36.0	36.0	36.0
9	35.084290510690934	36.0	36.0	36.0	36.0	36.0
10	34.909644454161814	36.0	36.0	36.0	32.0	36.0
11	35.10631632228249	36.0	36.0	36.0	36.0	36.0
12	35.025847532504095	36.0	36.0	36.0	36.0	36.0
13	35.06653867695376	36.0	36.0	36.0	36.0	36.0
14	34.99155986713829	36.0	36.0	36.0	36.0	36.0
15	34.98564159086621	36.0	36.0	36.0	36.0	36.0
16	34.96427170159428	36.0	36.0	36.0	36.0	36.0
17	34.90250119054394	36.0	36.0	36.0	32.0	36.0
18	34.87358669705674	36.0	36.0	36.0	32.0	36.0
19	34.85647880532285	36.0	36.0	36.0	32.0	36.0
20	34.819974781434105	36.0	36.0	36.0	32.0	36.0
21	34.823763556476706	36.0	36.0	36.0	32.0	36.0
22	34.868078746619304	36.0	36.0	36.0	32.0	36.0
23	34.89578622323523	36.0	36.0	36.0	32.0	36.0
24	34.854502053126716	36.0	36.0	36.0	32.0	36.0
25	34.848311224657884	36.0	36.0	36.0	32.0	36.0
26	34.80696715296767	36.0	36.0	36.0	32.0	36.0
27	34.82266136737341	36.0	36.0	36.0	32.0	36.0
28	34.8069012612278	36.0	36.0	36.0	32.0	36.0
29	34.73624734561116	36.0	36.0	36.0	32.0	36.0
30	34.66626133263049	36.0	36.0	36.0	32.0	36.0
31	34.57890985111462	36.0	36.0	36.0	32.0	36.0
32	34.45671661460221	36.0	36.0	36.0	32.0	36.0
33	34.26003276616519	36.0	36.0	36.0	32.0	36.0
34	34.093257777471614	36.0	36.0	36.0	32.0	36.0
35	33.94314441372824	36.0	36.0	36.0	32.0	36.0
36	33.88488713044468	36.0	36.0	36.0	32.0	36.0
37	33.82131657686421	36.0	36.0	36.0	32.0	36.0
38	33.70561068165005	36.0	36.0	36.0	27.0	36.0
39	33.59866239768061	36.0	36.0	36.0	21.0	36.0
40	33.46391378964361	36.0	36.0	36.0	21.0	36.0
41	33.31612760234934	36.0	36.0	36.0	14.0	36.0
42	33.13458986884549	36.0	36.0	36.0	14.0	36.0
43	32.96535891530216	36.0	36.0	36.0	14.0	36.0
44	32.69817989044001	36.0	36.0	36.0	14.0	36.0
45	32.40682758228231	36.0	36.0	36.0	14.0	36.0
46	32.0749338836292	36.0	36.0	36.0	14.0	36.0
47	31.70487688727421	36.0	36.0	36.0	14.0	36.0
48	31.417229491944735	36.0	32.0	36.0	14.0	36.0
49	31.131424070252574	36.0	32.0	36.0	14.0	36.0
50	30.63971594669957	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	2.0
20	2.0
21	27.0
22	66.0
23	215.0
24	566.0
25	1353.0
26	2772.0
27	5203.0
28	8613.0
29	11760.0
30	14549.0
31	18335.0
32	24175.0
33	34033.0
34	55298.0
35	156909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.15112130860773	20.010423811820242	13.782495784047374	18.055959095524653
2	14.309675061923633	23.540564096573906	40.77435238514552	21.375408456356944
3	17.367070106236092	33.896711083955566	29.792529628997332	18.943689180811013
4	9.228737184805365	16.83593855295749	37.31868539988798	36.61663886234916
5	9.765455356848697	43.20221875458621	30.29402691378066	16.73829897478443
6	25.12010303102911	40.0793698334731	19.82778243680364	14.97274469869414
7	24.973867934982824	36.422557737637064	21.24169988708552	17.361874440294596
8	30.81996280111776	27.930310499848748	19.62375816533436	21.625968533699133
9	30.61857738881451	12.153205203082553	22.375471353394495	34.85274605470845
10	18.0849406972565	27.38259254822092	29.478854678327544	25.05361207619504
11	31.447132361530006	25.580670957616636	24.345500342936553	18.626696337916805
12	26.99584582530902	29.458998864865027	28.637149163923674	14.908006145902283
13	28.79477656643105	22.672816580807474	30.206661075835626	18.325745776925842
14	24.551561783988905	21.354314860683896	32.854520023601225	21.239603331725974
15	22.74461763331616	27.041525393269193	32.384055974217354	17.82980099919729
16	19.41781304005343	28.91496620033006	29.548129711662007	22.119091047954498
17	16.70295704158068	26.52801447222214	31.121267757075127	25.647760729122055
18	17.34180741042467	25.366522803034613	37.29532378302449	19.996346003516223
19	20.812505054195956	23.735103225400668	38.31634624312255	17.136045477280827
20	23.674515841115618	21.41021570753389	36.29529349043663	18.619974960913865
21	26.608582099610338	20.54983661843591	35.10112884530716	17.74045243664659
22	22.69850635406028	22.616141679221037	35.5860321491789	19.09931981753978
23	20.72320046004966	24.10977695378837	37.40928545542221	17.757737130739766
24	19.7393682180178	25.790626001479566	36.72895432803903	17.7410514524636
25	20.206065144140773	25.687757394234367	36.859004118307745	17.247173343317108
26	19.09909424196679	26.341264706587115	37.78544557065152	16.774195480794575
27	18.281450774718973	27.358527300120112	36.89836849246547	17.46165343269545
28	17.084150779506686	27.04116933491082	38.34753568058946	17.527144204993036
29	17.341428276190506	26.90649981579712	37.38000605024096	18.372065857771414
30	17.56620349431044	27.709468425104163	36.76634007973474	17.957988000850662
31	17.809569168740996	28.915687913180747	34.58182439050692	18.692918527571344
32	16.50008236862205	29.248348883530767	35.01924430533299	19.23232444251419
33	18.32637938316016	28.252307084043455	34.525239394601975	18.89607413819441
34	18.879786740149463	27.868749344795052	34.84102855944768	18.410435355607806
35	19.701199276360718	28.79760863574826	33.13315442030982	18.3680376675812
36	18.200334879931464	29.90555667049073	33.49757826096072	18.396530188617078
37	18.780532367679346	31.23818766155385	32.529120877475165	17.452159093291638
38	17.51067870429724	31.832423721401142	33.49219091666117	17.16470665764044
39	17.34263849160589	32.77222829586809	31.921884594072452	17.963248618453566
40	17.490078622238862	33.225608386372144	32.67001123174841	16.614301759640586
41	17.53967160477557	33.40861303636788	31.748366110954695	17.303349247901853
42	17.401787532797393	34.44055734601699	30.79750080870284	17.360154312482777
43	17.072907743639817	34.204220753937115	30.038277913224746	18.68459358919832
44	17.689607456705225	33.3580931728736	29.860965513936392	19.09133385648478
45	18.09370628951727	33.46991221212857	29.201223219607453	19.23515827874671
46	19.85180037978399	33.58482541317982	27.273761958103954	19.28961224893223
47	19.496452222565004	33.90950397009623	26.86366890206098	19.730374905277788
48	20.01833026830481	34.71369438770315	25.89090495216758	19.37707039182446
49	19.192639227502262	34.6111453884353	26.37071025943608	19.82550512462636
50	18.012920925969745	34.973657963860944	26.91500163233766	20.098419477831655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	96.0
2	51.0
3	150.0
4	249.0
5	301.0
6	353.0
7	393.0
8	433.0
9	679.0
10	925.0
11	1416.0
12	1907.0
13	3522.0
14	5137.0
15	7712.5
16	10288.0
17	12031.0
18	13774.0
19	14184.5
20	14595.0
21	14327.5
22	14060.0
23	14000.5
24	13941.0
25	10871.0
26	7801.0
27	7436.0
28	7071.0
29	7634.0
30	8197.0
31	9155.0
32	10113.0
33	11751.5
34	13390.0
35	13991.0
36	14592.0
37	15441.0
38	16290.0
39	15763.5
40	15237.0
41	16546.0
42	17855.0
43	17045.5
44	16236.0
45	16685.5
46	17135.0
47	16788.0
48	16441.0
49	15572.5
50	14704.0
51	13857.5
52	13011.0
53	12442.5
54	11874.0
55	11937.0
56	12000.0
57	11607.0
58	11214.0
59	10195.0
60	9176.0
61	8155.0
62	7134.0
63	6045.0
64	4956.0
65	4523.0
66	4090.0
67	3605.0
68	3120.0
69	2784.5
70	2449.0
71	1928.0
72	1407.0
73	1214.0
74	1021.0
75	793.0
76	565.0
77	482.5
78	400.0
79	314.0
80	228.0
81	182.0
82	136.0
83	109.5
84	83.0
85	63.5
86	44.0
87	33.0
88	22.0
89	17.5
90	13.0
91	10.5
92	8.0
93	6.0
94	4.0
95	5.0
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008985237255189724
2	5.990158170126482E-4
3	5.990158170126482E-4
4	0.0
5	0.0
6	2.995079085063241E-4
7	0.0
8	0.0
9	5.990158170126482E-4
10	2.995079085063241E-4
11	0.0
12	0.0
13	2.995079085063241E-4
14	0.0
15	0.0038936028105822137
16	5.990158170126482E-4
17	0.0
18	0.0
19	0.0
20	8.985237255189724E-4
21	0.0
22	0.0
23	0.0011980316340252965
24	0.0
25	0.0017970474510379448
26	0.00509163444460751
27	0.005391142353113834
28	0.004792126536101186
29	0.002995079085063241
30	0.007188189804151779
31	0.011381300523240316
32	0.004792126536101186
33	0.005391142353113834
34	0.004792126536101186
35	0.0038936028105822137
36	0.008985237255189724
37	0.004193110719088538
38	0.011680808431746641
39	0.004792126536101186
40	0.0017970474510379448
41	0.004492618627594862
42	0.0038936028105822137
43	0.002096555359544269
44	0.002096555359544269
45	0.002396063268050593
46	0.002096555359544269
47	0.002995079085063241
48	0.002096555359544269
49	8.985237255189724E-4
50	0.0011980316340252965
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	333881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.02059424547226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23332520075562	26.018872980576642
2	12.972418891542118	9.345484745490753
3	5.198197661900238	5.617265063612132
4	2.6493274713066404	3.8172139946927817
5	1.5702417171614313	2.828051988059277
6	1.005326520382048	2.1727475204936546
7	0.6865198013197344	1.731019584337427
8	0.4901232101180827	1.4123623425561438
9	0.3544847349017682	1.1491875721899352
>10	2.3607321303458875	16.718948625640774
>50	0.2820895865109673	7.0638672838846395
>100	0.1730815162073192	12.094328718324148
>500	0.0149782081333257	3.455650949034922
>1k	0.00915334941481015	6.574998631106778
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4166	1.2477499468373463	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	2889	0.8652783476747703	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2613	0.7826141649270248	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2408	0.7212150436832284	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	1694	0.507366397009713	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1623	0.48610133550576407	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1526	0.4570490683806506	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1375	0.41182337419619564	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	1364	0.4085287872026261	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1144	0.3426370473312348	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	1134	0.33964196824617154	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	914	0.27375022837478025	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	759	0.22732650255630002	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	751	0.2249304392882494	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	721	0.21594520203305967	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	706	0.21145258340546483	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.21055405967994584	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	694	0.20785848850338892	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	659	0.1973757117056676	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	634	0.18988801399300947	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	620	0.18569490327392094	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	586	0.17551163438470593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	586	0.17551163438470593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	562	0.16832344458055415	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	549	0.16442984176997194	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	543	0.162632794318934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	520	0.15574411242328853	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	516	0.15454608078926324	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	506	0.1515510017042	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	483	0.14466231980855454	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	478	0.14316478026602292	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	463	0.13867216163842808	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	460	0.13777363791290909	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	446	0.13358052719382055	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	431	0.1290879085662257	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	426	0.12759036902369406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	426	0.12759036902369406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	419	0.1254938136641498	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	411	0.12309775039609921	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	410	0.12279824248759288	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA	410	0.12279824248759288	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	406	0.12160021085356759	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	389	0.11650857640896008	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	387	0.11590956059194743	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA	380	0.11381300523240316	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	362	0.10842186287928933	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	347	0.10392924425169446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.995079085063241E-4	0.0
4	0.0	0.0	0.0	2.995079085063241E-4	0.0
5	0.0	0.0	0.0	2.995079085063241E-4	0.0
6	0.0	0.0	0.0	2.995079085063241E-4	0.0
7	0.0	0.0	0.0	2.995079085063241E-4	0.0
8	0.0	0.0	0.0	2.995079085063241E-4	0.0
9	0.0	0.0	0.0	2.995079085063241E-4	0.0
10	0.0	0.0	0.0	2.995079085063241E-4	0.0
11	0.0	0.0	0.0	2.995079085063241E-4	0.0
12	0.0	0.0	0.0	2.995079085063241E-4	0.0
13	0.0	0.0	0.0	5.990158170126482E-4	0.0
14	0.0	0.0	0.0	5.990158170126482E-4	0.0
15	0.0	0.0	0.0	5.990158170126482E-4	0.0
16	0.0	0.0	0.0	8.985237255189723E-4	0.0
17	0.0	0.0	0.0	0.0011980316340252965	0.0
18	0.0	0.0	0.0	0.002096555359544269	0.0
19	0.0	0.0	0.0	0.002396063268050593	0.0
20	0.0	0.0	0.0	0.003294586993569565	0.0
21	0.0	0.0	0.0	0.004193110719088538	0.0
22	0.0	0.0	0.0	0.008985237255189724	0.0
23	0.0	0.0	0.0	0.02036653777843004	0.0
24	0.0	0.0	0.0	0.041332091373872726	0.0
25	0.0	0.0	0.0	0.04732224954399921	0.0
26	0.0	0.0	0.0	0.05540896307366996	0.0
27	0.0	0.0	0.0	0.06319616869483438	0.0
28	0.0	0.0	0.0	0.08266418274774545	0.0
29	0.0	0.0	0.0	0.11441202104941581	0.0
30	0.0	0.0	0.0	0.18659342699943993	0.0
31	0.0	0.0	0.0	0.3129857643891087	0.0
32	0.0	0.0	0.0	0.42949434079806875	0.0
33	0.0	0.0	0.0	0.5124580314543206	0.0
34	0.0	0.0	0.0	0.5918276272084965	0.0
35	0.0	0.0	0.0	0.7179204566896589	0.0
36	0.0	0.0	0.0	0.8784566956490486	0.0
37	0.0	0.0	0.0	1.0554658695762862	0.0
38	0.0	0.0	0.0	1.2774012297794723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATAGC	25	0.0023507942	35.19868	35
CATTATA	25	0.0023507942	35.19868	2
AGACCGT	25	0.0023507942	35.19868	6
AGGATAG	25	0.0023507942	35.19868	34
GTAACGT	25	0.0023507942	35.19868	43
GATAGCA	30	0.0057401857	29.332235	36
AGGTAGT	40	7.022939E-4	27.498972	15
CGACGGT	40	7.022939E-4	27.498972	7
TCTAGTA	40	7.022939E-4	27.498972	2
TGTCTAA	40	7.022939E-4	27.498972	34
CCTAATA	50	8.325809E-5	26.399012	2
GTTATAT	80	1.3043791E-7	24.749075	1
CAAGTCG	45	0.0013964018	24.44353	13
TTGCCGC	45	0.0013964018	24.44353	33
ATACCGT	45	0.0013964018	24.44353	6
CTCCCGA	55	1.5906553E-4	23.999102	1
TACCGTC	75	2.057497E-6	23.465786	7
CGACCGC	60	2.8645727E-4	22.002472	21
TAGCCAA	80	3.603749E-6	21.999178	11
CTAGTAG	60	2.867477E-4	21.999178	3
>>END_MODULE