FastQCFastQC Report
Thu 2 Feb 2017
SRR4064090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920573
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23200.25201695031246846No Hit
TATCAACGCAGAGTACTTTTTTTTT18530.2012876762625017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14970.16261610974903673No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9600.10428287599136624No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9370.10178443208740642No Hit
GCGCAAGACGGACCAGAGCGAAAGC9280.10080678012498738No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9230.10026364014586568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT902.2901077E-914.7746931
CGAACGT602.5671212E-514.2493494
TCGAACG602.5697544E-514.24783
GTGACCG551.958642E-413.81839
CGAGCCG1600.013.65710915
GTTATTC1001.0071744E-813.2979453
ATTCGAA655.4584587E-513.1510981
GCATCGC1251.0186341E-1012.92081314
CATCGCC1301.9645086E-1012.42385815
AGGCCCG1700.012.29422310
ACGGTAT1405.2750693E-1112.2143919
TTATACA701.0904333E-412.2137274
ATGGCGA1800.012.13965317
TTTCGTT550.003065365112.0916717
TAATAGT550.003067862212.0903554
TTAGCCT550.003067862212.0903554
CGGTCCA1900.012.00010210
CGCATCG1353.6925485E-1011.963716513
TGGCGAG1701.8189894E-1211.736032518
TTAATAT2350.011.7215933
Produced by FastQC (version 0.11.5)