##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920573
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226855447639675	32.0	32.0	32.0	32.0	32.0
2	30.824738505257052	32.0	32.0	32.0	32.0	32.0
3	30.849879368610637	32.0	32.0	32.0	32.0	32.0
4	30.882397159160654	32.0	32.0	32.0	32.0	32.0
5	30.799635661702006	32.0	32.0	32.0	32.0	32.0
6	34.445250946964556	36.0	36.0	36.0	32.0	36.0
7	34.36768295398627	36.0	36.0	36.0	32.0	36.0
8	34.334303743429366	36.0	36.0	36.0	32.0	36.0
9	34.457235873743855	36.0	36.0	36.0	32.0	36.0
10	34.16958025056134	36.0	36.0	36.0	32.0	36.0
11	34.444460135154955	36.0	36.0	36.0	32.0	36.0
12	34.26678058122496	36.0	36.0	36.0	32.0	36.0
13	34.350493659927025	36.0	36.0	36.0	32.0	36.0
14	34.238437364554464	36.0	36.0	36.0	32.0	36.0
15	34.18798617817381	36.0	36.0	36.0	32.0	36.0
16	34.1863111344782	36.0	36.0	36.0	32.0	36.0
17	34.11032150627924	36.0	36.0	36.0	32.0	36.0
18	34.10570155761683	36.0	36.0	36.0	32.0	36.0
19	34.12636477498254	36.0	36.0	36.0	32.0	36.0
20	34.096010854109345	36.0	36.0	36.0	32.0	36.0
21	34.076671812012734	36.0	36.0	36.0	32.0	36.0
22	34.06387652038459	36.0	36.0	36.0	32.0	36.0
23	34.00531082271585	36.0	36.0	36.0	32.0	36.0
24	34.00137957554697	36.0	36.0	36.0	32.0	36.0
25	33.591402311386496	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	7.0
5	8.0
6	43.0
7	11.0
8	37.0
9	48.0
10	91.0
11	29.0
12	61.0
13	44.0
14	83.0
15	148.0
16	249.0
17	340.0
18	443.0
19	658.0
20	942.0
21	1431.0
22	2270.0
23	3515.0
24	5169.0
25	7543.0
26	10551.0
27	13950.0
28	18771.0
29	25328.0
30	33203.0
31	44504.0
32	62702.0
33	88179.0
34	188251.0
35	411962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75490612642316	17.098824529165086	11.247550876033849	25.89871846837791
2	16.57077862681664	20.00199887454672	37.60752098275101	25.819701515885622
3	18.860775444405338	24.128787385015144	28.777391054971126	28.233046115608396
4	12.344737533920382	15.500392161974846	36.32093406247216	35.83393624163261
5	14.270132505657319	37.12885216444737	33.77682948954306	14.824185840352246
6	33.6853289529434	35.9836213396258	17.177386236971273	13.153663470459529
7	29.941469128143165	30.61511198225929	21.032325078310983	18.411093811286563
8	28.172411769691674	32.89552323142757	19.505314790787843	19.426750208092912
9	27.596366608269246	13.765742306538629	18.533319132472297	40.10457195271982
10	15.618125298900049	26.65938437459241	32.08360506064954	25.638885265858008
11	37.05259519782976	20.900482999378315	23.03202751053165	19.014894292260273
12	24.715784027382995	23.65957345049441	29.275087582759323	22.349554939363273
13	29.41439257740744	19.548544459685957	25.51646626855159	25.520596694355014
14	23.555435263477694	19.73971258164553	25.595364262165717	31.109487892711062
15	25.010108256162777	27.38152689013521	22.555541063432024	25.05282379026999
16	25.383567704115627	25.7835759651558	24.171803548116756	24.661052782611815
17	23.766615326589196	25.90148574633509	25.514641704592023	24.817257222483693
18	24.603672423558212	25.098735382651927	26.433361634033893	23.86423055975597
19	25.411776727440333	24.847812191547924	25.578857200005217	24.161553881006526
20	25.74654489596911	24.390376476701626	25.18690120679965	24.67617742052962
21	26.65650634078255	24.112692653040817	24.691439889207516	24.539361116969122
22	25.876386722253326	24.25390130288025	25.1734727710529	24.69623920381352
23	24.42535389806996	24.27510825405212	25.648301256649347	25.651236591228567
24	24.672244133006917	25.059463502237218	25.509190209285435	24.759102155470426
25	24.7863833228971	24.72757079245808	25.535617999025952	24.95042788561887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	209.0
1	209.0
2	354.5
3	500.0
4	500.0
5	500.0
6	1242.5
7	1985.0
8	1985.0
9	1985.0
10	1940.0
11	1895.0
12	1895.0
13	1895.0
14	2083.5
15	2272.0
16	2272.0
17	2272.0
18	4011.5
19	5751.0
20	5751.0
21	5751.0
22	9643.0
23	13535.0
24	13535.0
25	13535.0
26	20184.5
27	26834.0
28	26834.0
29	26834.0
30	33782.0
31	40730.0
32	40730.0
33	40730.0
34	50495.0
35	60260.0
36	60260.0
37	60260.0
38	68561.5
39	76863.0
40	76863.0
41	76863.0
42	87429.5
43	97996.0
44	97996.0
45	97996.0
46	109217.0
47	120438.0
48	120438.0
49	120438.0
50	123735.0
51	127032.0
52	127032.0
53	127032.0
54	117078.5
55	107125.0
56	107125.0
57	107125.0
58	96914.0
59	86703.0
60	86703.0
61	86703.0
62	75504.5
63	64306.0
64	64306.0
65	64306.0
66	53172.0
67	42038.0
68	42038.0
69	42038.0
70	31555.0
71	21072.0
72	21072.0
73	21072.0
74	16222.5
75	11373.0
76	11373.0
77	11373.0
78	8991.5
79	6610.0
80	6610.0
81	6610.0
82	4529.0
83	2448.0
84	2448.0
85	2448.0
86	1853.5
87	1259.0
88	1259.0
89	1259.0
90	931.5
91	604.0
92	604.0
93	604.0
94	433.5
95	263.0
96	263.0
97	263.0
98	367.5
99	472.0
100	472.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037585286555221586
2	0.005974539770338691
3	0.0018466759290137772
4	0.003801979853851894
5	0.009124751649244547
6	0.011623195553204364
7	0.022160111148165327
8	0.03378330670136969
9	0.04790494615853387
10	0.057898721774373135
11	0.05311908995810218
12	0.06365600555306315
13	0.06202658561569806
14	0.0640905175363605
15	0.057898721774373135
16	0.06430777352800919
17	0.06126618964492767
18	0.0749533171187945
19	0.07136859325659127
20	0.07625685306868657
21	0.07115133726494259
22	0.07473606112714581
23	0.08103648488495752
24	0.07397566515637542
25	0.07614822507286223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	920573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.63874750516816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3123991505793	40.39662762046364
2	14.607712662008629	15.670788222110577
3	4.6387245684438145	7.464461276183338
4	1.9884137697651856	4.266240965289375
5	0.9557422281289573	2.5632408027317988
6	0.5874954640844688	1.890751251507505
7	0.3638081525431044	1.3659949544206895
8	0.2595690432391774	1.1138366696371451
9	0.18087167437664764	0.8731557065453397
>10	0.9341962951683704	8.993912011540564
>50	0.08116290689232553	3.0854697126377673
>100	0.08075095402587719	8.535546207736502
>500	0.008542922027876397	3.1613618884228103
>1k	6.102087162768855E-4	0.6186127107729615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2320	0.25201695031246846	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1853	0.2012876762625017	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1497	0.16261610974903673	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	960	0.10428287599136624	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	937	0.10178443208740642	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	928	0.10080678012498738	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	923	0.10026364014586568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0862799582433983E-4
2	0.0	0.0	0.0	0.0	1.0862799582433983E-4
3	0.0	0.0	0.0	0.0	1.0862799582433983E-4
4	0.0	0.0	0.0	0.0	1.0862799582433983E-4
5	0.0	0.0	0.0	0.0	2.1725599164867967E-4
6	0.0	0.0	0.0	0.0	2.1725599164867967E-4
7	0.0	0.0	0.0	0.0	2.1725599164867967E-4
8	0.0	0.0	0.0	0.0	2.1725599164867967E-4
9	0.0	0.0	0.0	0.0	3.2588398747301954E-4
10	0.0	0.0	0.0	0.0	3.2588398747301954E-4
11	0.0	0.0	0.0	3.2588398747301954E-4	3.2588398747301954E-4
12	0.0	0.0	0.0	3.2588398747301954E-4	6.517679749460391E-4
13	0.0	0.0	0.0	3.2588398747301954E-4	8.690239665947187E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	90	2.2901077E-9	14.774693	1
CGAACGT	60	2.5671212E-5	14.249349	4
TCGAACG	60	2.5697544E-5	14.2478	3
GTGACCG	55	1.958642E-4	13.8183	9
CGAGCCG	160	0.0	13.657109	15
GTTATTC	100	1.0071744E-8	13.297945	3
ATTCGAA	65	5.4584587E-5	13.151098	1
GCATCGC	125	1.0186341E-10	12.920813	14
CATCGCC	130	1.9645086E-10	12.423858	15
AGGCCCG	170	0.0	12.294223	10
ACGGTAT	140	5.2750693E-11	12.214391	9
TTATACA	70	1.0904333E-4	12.213727	4
ATGGCGA	180	0.0	12.139653	17
TTTCGTT	55	0.0030653651	12.09167	17
TAATAGT	55	0.0030678622	12.090355	4
TTAGCCT	55	0.0030678622	12.090355	4
CGGTCCA	190	0.0	12.000102	10
CGCATCG	135	3.6925485E-10	11.9637165	13
TGGCGAG	170	1.8189894E-12	11.7360325	18
TTAATAT	235	0.0	11.721593	3
>>END_MODULE