FastQCFastQC Report
Thu 2 Feb 2017
SRR4064090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920573
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11000.11949079540677382No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9690.10526052795378531No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9460.1027620840498255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1350.024.4423625
TTAACGG904.0765553E-722.0017135
GTATCAA18850.021.359751
TCCAATA3150.020.9505982
TAGGACG1401.8553692E-1020.4268324
GTATTAA1352.593879E-919.5570771
GTATAGG901.0008618E-519.5570761
TAGCGTA2250.019.553897
GTATTAG1950.019.180981
ACTGCGC708.1259635E-418.8555378
AGCGTAT2450.018.8555368
ATAGCGT2350.018.721816
GTAGGAC2950.018.6424793
ATAATAC1557.221388E-1018.450043
ATATACT1557.221388E-1018.450044
AACGCAG21850.018.3233836
CGTATAT2550.018.11807310
AATAGCG2450.017.9576535
CCAATAC1254.8537004E-717.5985013
CATATAC1509.596988E-917.59853
Produced by FastQC (version 0.11.5)