##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920573
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301307989697722	32.0	32.0	32.0	32.0	32.0
2	31.39543523435947	32.0	32.0	32.0	32.0	32.0
3	31.470838271380977	32.0	32.0	32.0	32.0	32.0
4	31.556195977939826	32.0	32.0	32.0	32.0	32.0
5	31.50214703233747	32.0	32.0	32.0	32.0	32.0
6	35.079138753797906	36.0	36.0	36.0	36.0	36.0
7	35.10321723535233	36.0	36.0	36.0	36.0	36.0
8	35.05191331920445	36.0	36.0	36.0	36.0	36.0
9	35.15088645875993	36.0	36.0	36.0	36.0	36.0
10	35.00689461889497	36.0	36.0	36.0	36.0	36.0
11	35.16386424542106	36.0	36.0	36.0	36.0	36.0
12	35.060990274535534	36.0	36.0	36.0	36.0	36.0
13	35.10800664368823	36.0	36.0	36.0	36.0	36.0
14	35.048140668909475	36.0	36.0	36.0	36.0	36.0
15	35.015959625146515	36.0	36.0	36.0	36.0	36.0
16	35.03421999124458	36.0	36.0	36.0	36.0	36.0
17	34.99738315158059	36.0	36.0	36.0	36.0	36.0
18	34.99829997186535	36.0	36.0	36.0	36.0	36.0
19	34.99862259701295	36.0	36.0	36.0	36.0	36.0
20	34.985774077666846	36.0	36.0	36.0	36.0	36.0
21	34.97232810434371	36.0	36.0	36.0	36.0	36.0
22	34.967350769575035	36.0	36.0	36.0	36.0	36.0
23	34.91725371046077	36.0	36.0	36.0	32.0	36.0
24	34.89831659194871	36.0	36.0	36.0	32.0	36.0
25	34.86365013964129	36.0	36.0	36.0	32.0	36.0
26	34.79335479098344	36.0	36.0	36.0	32.0	36.0
27	34.8007154239805	36.0	36.0	36.0	32.0	36.0
28	34.77848796347492	36.0	36.0	36.0	32.0	36.0
29	34.74893571721091	36.0	36.0	36.0	32.0	36.0
30	34.72855710519426	36.0	36.0	36.0	32.0	36.0
31	34.722409846910566	36.0	36.0	36.0	32.0	36.0
32	34.671133087761646	36.0	36.0	36.0	32.0	36.0
33	34.65103473597422	36.0	36.0	36.0	32.0	36.0
34	34.63400947018867	36.0	36.0	36.0	32.0	36.0
35	34.599057326252236	36.0	36.0	36.0	32.0	36.0
36	34.558517358210594	36.0	36.0	36.0	32.0	36.0
37	34.55924625206257	36.0	36.0	36.0	32.0	36.0
38	34.514110233517606	36.0	36.0	36.0	32.0	36.0
39	34.50184070138924	36.0	36.0	36.0	32.0	36.0
40	34.49097464296693	36.0	36.0	36.0	32.0	36.0
41	34.47025711160332	36.0	36.0	36.0	32.0	36.0
42	34.401733485557365	36.0	36.0	36.0	32.0	36.0
43	34.39124110744069	36.0	36.0	36.0	32.0	36.0
44	34.32492588854985	36.0	36.0	36.0	32.0	36.0
45	34.28850726666978	36.0	36.0	36.0	32.0	36.0
46	34.265561775111806	36.0	36.0	36.0	32.0	36.0
47	34.22781246028289	36.0	36.0	36.0	32.0	36.0
48	34.17884947744503	36.0	36.0	36.0	32.0	36.0
49	34.14441548904867	36.0	36.0	36.0	32.0	36.0
50	33.5660691764803	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	15.0
21	27.0
22	102.0
23	310.0
24	805.0
25	1674.0
26	3684.0
27	6598.0
28	10908.0
29	16357.0
30	23877.0
31	33292.0
32	47815.0
33	77259.0
34	160157.0
35	537688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.980866358926335	18.086027509769433	11.796000534507801	26.137105596796427
2	15.941444910701968	20.78366749260512	37.284114498750235	25.990773097942686
3	17.988548386921565	24.954131629459607	29.09146408362654	27.965855899992288
4	11.9870993392159	16.172753274319366	36.57113558620555	35.269011800259186
5	13.884721798271293	37.449610188436985	33.65414801433455	15.011519998957171
6	33.63256855271033	36.49666673546469	16.777123713703155	13.093640998121813
7	29.71185306526812	30.953472406286526	21.040831135424497	18.29384339302086
8	28.035364930320572	33.21637719116246	19.437893572807372	19.310364305709594
9	27.787759624576346	13.688950204223516	18.35371947510211	40.16957069609803
10	15.650028732292162	26.886907742728123	31.9605199838795	25.502543541100213
11	37.30155023012841	20.91740687593488	22.9338683624221	18.84717453151461
12	24.696466222667837	23.702954572858424	29.201377837499038	22.3992013669747
13	29.581205577842447	19.587082365184216	25.374468672161083	25.45724338481225
14	23.654532182165003	19.606722776708395	25.56779915096266	31.170945890163942
15	25.014448166929558	27.446085524457597	22.590197842117384	24.94926846649546
16	25.479703835023592	25.844369997653622	24.069053019440172	24.60687314788261
17	23.82744225607312	26.000653940534864	25.33726277003562	24.8346410333564
18	24.6636858982715	25.04377732008934	26.50613534252766	23.786401439111504
19	25.544797739663753	24.753332442581833	25.636262710860976	24.065607106893438
20	25.811914561723103	24.24701625404233	25.27290127540407	24.668167908830497
21	26.848169563956365	24.055886931291706	24.714715725966325	24.381227778785604
22	25.9530748783638	24.282376302585455	25.24112699373108	24.523421825319666
23	24.4352109845584	24.169613226659713	25.76960888150212	25.62556690727977
24	24.750615653178965	24.959834536758326	25.682867189460406	24.606682620602303
25	24.933518727730945	24.45337620578778	25.74074476405666	24.87236030242461
26	24.492387578962234	25.317233999793597	25.990755375949853	24.199623045294313
27	25.12797059066358	24.832269446532624	25.253985773320416	24.78577418948338
28	24.45075477765128	24.81836669071063	25.989966715044275	24.740911816593812
29	24.478795515773008	24.722668810289388	25.851438254975236	24.94709741896237
30	24.38921852560774	25.15279042129528	26.049979251354095	24.408011801742884
31	25.13918838910827	24.819041514804212	24.955812778716265	25.085957317371253
32	24.722961823536377	25.16965566987239	24.808237764531942	25.299144742059287
33	24.398415255609017	24.698134872741377	25.594469255111473	25.308980616538136
34	25.263754790603898	24.737983296978335	25.540545238074547	24.457716674343217
35	25.5642291330816	24.636086331185336	25.640270795221976	24.159413740511088
36	24.66417598319169	25.22658954890673	25.154454604414788	24.954779863486788
37	25.406743628488837	24.9981804217728	25.02533830576084	24.569737643977522
38	24.734847209201018	24.884981863255792	25.39241961223543	24.98775131530776
39	25.254633150107548	24.8208660134269	24.891150846242425	25.03334999022313
40	25.58342901898758	24.987588927911496	25.41298724999973	24.015994803101194
41	24.50507741041206	25.37890473935091	25.77877525126338	24.337242598973656
42	25.798752932997303	25.53934561571218	25.013144173112018	23.6487572781785
43	24.908995512509247	24.653824469697216	25.422487857921112	25.014692159872425
44	24.6266435505036	25.39030250888582	24.893652614472803	25.08940132613778
45	24.828691975788754	25.537062061953325	24.927218531248236	24.707027431009685
46	24.642259298325275	25.097412578524803	24.986068399001052	25.274259724148866
47	24.8863725243115	25.069740764237107	25.296126671822993	24.747760039628396
48	25.611776348449904	25.419284194243268	24.407939975471372	24.560999481835456
49	24.73779860543076	25.68166931176411	24.68152918134506	24.899002901460072
50	24.95081814063785	25.805947012860607	24.45937974913178	24.783855097369763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	53.0
2	23.0
3	93.5
4	164.0
5	179.5
6	195.0
7	175.5
8	156.0
9	233.0
10	310.0
11	469.0
12	628.0
13	1120.5
14	1613.0
15	2156.0
16	2699.0
17	2891.0
18	3083.0
19	3096.0
20	3109.0
21	3330.0
22	3551.0
23	4012.0
24	4473.0
25	5017.5
26	5562.0
27	6886.0
28	8210.0
29	9844.5
30	11479.0
31	13073.0
32	14667.0
33	17291.5
34	19916.0
35	23334.0
36	26752.0
37	31207.0
38	35662.0
39	38551.0
40	41440.0
41	45133.5
42	48827.0
43	49751.5
44	50676.0
45	56068.0
46	61460.0
47	64508.5
48	67557.0
49	69824.0
50	72091.0
51	69797.5
52	67504.0
53	67365.5
54	67227.0
55	66494.5
56	65762.0
57	63273.0
58	60784.0
59	55197.5
60	49611.0
61	43379.5
62	37148.0
63	32079.0
64	27010.0
65	23035.5
66	19061.0
67	16360.0
68	13659.0
69	12413.5
70	11168.0
71	8706.0
72	6244.0
73	5190.0
74	4136.0
75	3260.5
76	2385.0
77	2063.5
78	1742.0
79	1462.5
80	1183.0
81	980.5
82	778.0
83	600.0
84	422.0
85	290.5
86	159.0
87	112.5
88	66.0
89	54.5
90	43.0
91	37.5
92	32.0
93	28.5
94	25.0
95	19.5
96	14.0
97	13.0
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010862799582433984
2	4.3451198329735934E-4
3	4.3451198329735934E-4
4	0.0
5	0.0
6	4.3451198329735934E-4
7	1.0862799582433983E-4
8	0.0
9	0.0014121639457164179
10	6.517679749460391E-4
11	0.0
12	0.0
13	2.1725599164867967E-4
14	1.0862799582433983E-4
15	0.004453747828797933
16	5.431399791216992E-4
17	0.0
18	5.431399791216992E-4
19	2.1725599164867967E-4
20	2.1725599164867967E-4
21	0.0
22	0.0
23	8.690239665947187E-4
24	6.517679749460391E-4
25	0.0014121639457164179
26	0.004127863841324914
27	0.005322771795392652
28	0.004019235845500574
29	0.0014121639457164179
30	0.0033674678705545346
31	0.006300423757811711
32	0.0034760958663788747
33	0.0049968878079196324
34	0.002281187912311137
35	0.002715699895608496
36	0.008255727682649828
37	0.003258839874730195
38	0.007169447724406429
39	0.0035847238622032144
40	0.0026070718997841563
41	0.002715699895608496
42	0.0014121639457164179
43	0.0015207919415407578
44	5.431399791216992E-4
45	9.776519624190585E-4
46	4.3451198329735934E-4
47	0.002281187912311137
48	0.0017380479331894373
49	2.1725599164867967E-4
50	0.0010862799582433984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	920573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80104012475897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3370194509167	43.36022104836025
2	14.162165254578293	16.08851433757538
3	4.486858064266501	7.645746148274997
4	1.841767523805507	4.184572440806183
5	0.945193861355444	2.684399722226322
6	0.5075101632169882	1.729626308676667
7	0.3480254037872515	1.383774344746858
8	0.24575712784129297	1.1167408383567041
9	0.17795093570657752	0.9097018415376943
>10	0.8038065016222068	8.156326130708168
>50	0.0690897900522525	2.7384787902561163
>100	0.06889054092643782	7.663137629540502
>500	0.005772950249559971	2.2185269067208737
>1k	1.924316749853324E-4	0.12023351221328085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1100	0.11949079540677382	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	969	0.10526052795378531	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	946	0.1027620840498255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0862799582433983E-4
2	0.0	0.0	0.0	1.0862799582433983E-4	1.0862799582433983E-4
3	0.0	0.0	0.0	1.0862799582433983E-4	1.0862799582433983E-4
4	0.0	0.0	0.0	1.0862799582433983E-4	1.0862799582433983E-4
5	0.0	0.0	0.0	2.1725599164867967E-4	1.0862799582433983E-4
6	0.0	0.0	0.0	2.1725599164867967E-4	2.1725599164867967E-4
7	0.0	0.0	0.0	2.1725599164867967E-4	2.1725599164867967E-4
8	0.0	0.0	0.0	2.1725599164867967E-4	3.2588398747301954E-4
9	0.0	0.0	0.0	2.1725599164867967E-4	3.2588398747301954E-4
10	0.0	0.0	0.0	2.1725599164867967E-4	3.2588398747301954E-4
11	0.0	0.0	0.0	3.2588398747301954E-4	3.2588398747301954E-4
12	0.0	0.0	0.0	3.2588398747301954E-4	6.517679749460391E-4
13	0.0	0.0	0.0	3.2588398747301954E-4	8.690239665947187E-4
14	0.0	0.0	0.0	3.2588398747301954E-4	0.0010862799582433984
15	0.0	0.0	0.0	3.2588398747301954E-4	0.0013035359498920782
16	0.0	0.0	0.0	3.2588398747301954E-4	0.0013035359498920782
17	0.0	0.0	0.0	3.2588398747301954E-4	0.0013035359498920782
18	0.0	0.0	0.0	3.2588398747301954E-4	0.0013035359498920782
19	0.0	0.0	0.0	3.2588398747301954E-4	0.0013035359498920782
20	0.0	0.0	0.0	3.2588398747301954E-4	0.0014121639457164179
21	0.0	0.0	0.0	8.690239665947187E-4	0.0014121639457164179
22	0.0	0.0	0.0	0.0010862799582433984	0.0014121639457164179
23	0.0	0.0	0.0	0.0013035359498920782	0.0015207919415407578
24	0.0	0.0	0.0	0.001955303924838117	0.0015207919415407578
25	0.0	0.0	0.0	0.0024984439039598162	0.0015207919415407578
26	0.0	0.0	0.0	0.0028243278914328357	0.0017380479331894373
27	0.0	0.0	0.0	0.0032588398747301953	0.0017380479331894373
28	0.0	0.0	0.0	0.0049968878079196324	0.0018466759290137772
29	0.0	0.0	0.0	0.00792984369517681	0.0018466759290137772
30	0.0	0.0	0.0	0.01346987148221814	0.0018466759290137772
31	0.0	0.0	0.0	0.024875811043773823	0.0018466759290137772
32	0.0	0.0	0.0	0.038128426534343285	0.0018466759290137772
33	0.0	0.0	0.0	0.0507292740499667	0.0018466759290137772
34	0.0	0.0	0.0	0.06724072941526636	0.001955303924838117
35	0.0	0.0	0.0	0.08744553663859357	0.001955303924838117
36	0.0	0.0	0.0	0.11427665160720551	0.001955303924838117
37	0.0	0.0	0.0	0.15729333795364409	0.001955303924838117
38	0.0	0.0	0.0	0.2132367558031791	0.001955303924838117
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	135	0.0	24.442362	5
TTAACGG	90	4.0765553E-7	22.00171	35
GTATCAA	1885	0.0	21.35975	1
TCCAATA	315	0.0	20.950598	2
TAGGACG	140	1.8553692E-10	20.426832	4
GTATTAA	135	2.593879E-9	19.557077	1
GTATAGG	90	1.0008618E-5	19.557076	1
TAGCGTA	225	0.0	19.55389	7
GTATTAG	195	0.0	19.18098	1
ACTGCGC	70	8.1259635E-4	18.855537	8
AGCGTAT	245	0.0	18.855536	8
ATAGCGT	235	0.0	18.72181	6
GTAGGAC	295	0.0	18.642479	3
ATAATAC	155	7.221388E-10	18.45004	3
ATATACT	155	7.221388E-10	18.45004	4
AACGCAG	2185	0.0	18.323383	6
CGTATAT	255	0.0	18.118073	10
AATAGCG	245	0.0	17.957653	5
CCAATAC	125	4.8537004E-7	17.598501	3
CATATAC	150	9.596988E-9	17.5985	3
>>END_MODULE