FastQCFastQC Report
Thu 2 Feb 2017
SRR4064089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930025
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43740.47030993790489506No Hit
TATCAACGCAGAGTACTTTTTTTTT30540.32837826940136017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25230.27128303002607457No Hit
GTACATGGGGTGGTATCAACGCAAA17170.18461869304588588No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17060.18343592914168974No Hit
GGTATCAACGCAGAGTACTTTTTTT16290.17515658181231686No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15270.16418913470068008No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13280.1427918604338593No Hit
GTATCTGATCGTCTTCGAACCTCCG12720.13677051692158812No Hit
GATTAAGAGGGACGGCCGGGGGCAT12600.13548022902610143No Hit
GAATAGGACCGCGGTTCTATTTTGT12050.12956640950512083No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11970.1287062175747964No Hit
GCGCAAGACGGACCAGAGCGAAAGC11910.12806107362705305No Hit
CTATTGGAGCTGGAATTACCGCGGC11630.12505040187091745No Hit
GAACTACGACGGTATCTGATCGTCT11270.12117953818445741No Hit
GAATAACGCCGCCGCATCGCCAGTC11180.1202118222628424No Hit
GTCCTATTCCATTATTCCTAGCTGC10570.11365285879411843No Hit
ATCAGATACCGTCGTAGTTCCGACC10170.10935189914249618No Hit
GTATCAACGCAGAGTACATGGGGTG10160.10924437515120562No Hit
GGGTAGGCACACGCTGAGCCAGTCA9970.10720141931668505No Hit
GTACATGGGGAATAATTGCAATCCC9760.10494341549958333No Hit
CTTTAATATACGCTATTGGAGCTGG9500.10214779172602888No Hit
TCGTAGTTCCGACCATAAACGATGC9400.10107255181312332No Hit
CTCTTAATCATGGCCTCAGTTCCGA9360.10064245584796108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGC350.002172108516.2825785
CGCATCG2450.015.12441913
GTATTAC456.779639E-414.7717531
CGGACAT653.3753386E-614.612575
GCATCGC2450.014.34958114
CGAACGA1006.566552E-1014.25185716
TTAGACC602.5720386E-514.2464894
CTAAACC400.005289396714.2449573
CGCCAGT2550.014.15870818
GCTCGTA954.8821676E-914.0018249
ATCGCCA2700.013.724009516
AGACCGT500.001499716213.298876
GCTTAGG500.001503369213.2945781
CCGCATC2800.013.2345812
CGCCCCT1800.013.1919035
TTTTCGG1403.6379788E-1212.89453816
TCGCCAG2750.012.78348417
ATCACGA905.385082E-712.66912
CCGACCA2250.012.6683179
GGACCGT604.092208E-412.665596
Produced by FastQC (version 0.11.5)