##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064089_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	930025
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21378134996371	32.0	32.0	32.0	32.0	32.0
2	30.8230950780893	32.0	32.0	32.0	32.0	32.0
3	30.843013897475874	32.0	32.0	32.0	32.0	32.0
4	30.866915405499853	32.0	32.0	32.0	32.0	32.0
5	30.785870272304507	32.0	32.0	32.0	32.0	32.0
6	34.43113142119835	36.0	36.0	36.0	32.0	36.0
7	34.335507110023926	36.0	36.0	36.0	32.0	36.0
8	34.29611677105454	36.0	36.0	36.0	32.0	36.0
9	34.413210397569955	36.0	36.0	36.0	32.0	36.0
10	34.1268320744066	36.0	36.0	36.0	32.0	36.0
11	34.444005268675575	36.0	36.0	36.0	32.0	36.0
12	34.217001693502866	36.0	36.0	36.0	32.0	36.0
13	34.31262062847773	36.0	36.0	36.0	32.0	36.0
14	34.22193059326362	36.0	36.0	36.0	32.0	36.0
15	34.14840568801914	36.0	36.0	36.0	32.0	36.0
16	34.14954759280665	36.0	36.0	36.0	32.0	36.0
17	34.06875299051101	36.0	36.0	36.0	32.0	36.0
18	34.06722614983468	36.0	36.0	36.0	32.0	36.0
19	34.07714308755141	36.0	36.0	36.0	32.0	36.0
20	34.055671621730596	36.0	36.0	36.0	32.0	36.0
21	34.02393161474154	36.0	36.0	36.0	32.0	36.0
22	33.99103572484611	36.0	36.0	36.0	32.0	36.0
23	33.94925190183059	36.0	36.0	36.0	32.0	36.0
24	33.94102739173678	36.0	36.0	36.0	32.0	36.0
25	33.53972957716191	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	7.0
5	13.0
6	37.0
7	17.0
8	67.0
9	48.0
10	75.0
11	31.0
12	47.0
13	50.0
14	116.0
15	178.0
16	294.0
17	355.0
18	499.0
19	620.0
20	981.0
21	1428.0
22	2354.0
23	3607.0
24	5205.0
25	7455.0
26	10751.0
27	14019.0
28	19009.0
29	25756.0
30	33804.0
31	46392.0
32	66061.0
33	93506.0
34	198281.0
35	398956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81928719099383	17.52711165419276	11.572981496739718	25.080619658073687
2	16.038607240362975	20.234822001739822	38.58971312317133	25.136857634725875
3	18.807667452680565	24.91083419622821	28.886494646826	27.395003704265225
4	12.021084075007177	15.588968065282351	36.73981791221635	35.650129947494115
5	13.939931608490872	37.45499709658688	33.55786394821171	15.04720734671054
6	32.996377002515345	36.441504560202084	17.350921019945307	13.211197417337264
7	29.321183167725845	31.136394678907177	21.019800840817844	18.522621312549138
8	27.99206618966281	33.02201159093549	19.7116894627468	19.27423275665489
9	27.78017429170463	13.67095851831612	18.945751411104453	39.603115778874795
10	16.01535897681414	26.630331289558022	31.40736469178226	25.94694504184557
11	37.09757065812024	21.072047560491484	23.086977991653676	18.743403789734604
12	24.988917962798784	23.882973687155165	29.34727876810783	21.780829581938217
13	29.662059148525483	19.63164242761369	25.77427070343643	24.932027720424394
14	23.45341745893067	20.328296413615607	25.660638430650177	30.557647696803542
15	24.59160136161569	28.35336595358285	22.92207289205624	24.132959792745222
16	24.842884026300585	25.963488625567432	25.15426469939499	24.039362648736994
17	23.016572362468114	26.324804532626672	26.0575465415159	24.601076563389313
18	23.99151200027547	25.03238955270135	27.901178506249785	23.074919940773388
19	25.344577312399185	24.62879051184352	26.15258138416295	23.87405079159435
20	25.597173768473702	24.329760011363465	25.863397374781282	24.20966884538155
21	27.164723541849366	23.854757920901278	24.830283694429948	24.150234842819405
22	25.681345441358612	24.749677445736097	25.587834296960832	23.981142815944455
23	23.81003624342496	24.586773302111762	26.39593143880371	25.207259015659574
24	24.50351007829347	25.274285394544854	26.27275309795077	23.94945142921091
25	24.393015440537948	24.94514729793276	26.33500016679095	24.326837094738337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	419.0
3	678.0
4	678.0
5	678.0
6	1859.0
7	3040.0
8	3040.0
9	3040.0
10	2943.5
11	2847.0
12	2847.0
13	2847.0
14	2668.5
15	2490.0
16	2490.0
17	2490.0
18	4383.0
19	6276.0
20	6276.0
21	6276.0
22	10728.5
23	15181.0
24	15181.0
25	15181.0
26	24056.0
27	32931.0
28	32931.0
29	32931.0
30	41802.5
31	50674.0
32	50674.0
33	50674.0
34	58539.0
35	66404.0
36	66404.0
37	66404.0
38	72465.0
39	78526.0
40	78526.0
41	78526.0
42	88570.0
43	98614.0
44	98614.0
45	98614.0
46	109679.0
47	120744.0
48	120744.0
49	120744.0
50	120878.0
51	121012.0
52	121012.0
53	121012.0
54	108135.5
55	95259.0
56	95259.0
57	95259.0
58	87831.0
59	80403.0
60	80403.0
61	80403.0
62	72010.0
63	63617.0
64	63617.0
65	63617.0
66	53301.5
67	42986.0
68	42986.0
69	42986.0
70	32384.0
71	21782.0
72	21782.0
73	21782.0
74	17047.0
75	12312.0
76	12312.0
77	12312.0
78	10050.5
79	7789.0
80	7789.0
81	7789.0
82	5323.5
83	2858.0
84	2858.0
85	2858.0
86	2289.0
87	1720.0
88	1720.0
89	1720.0
90	1230.0
91	740.0
92	740.0
93	740.0
94	534.0
95	328.0
96	328.0
97	328.0
98	491.0
99	654.0
100	654.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0377409209429854
2	0.0047310556167844955
3	0.0017203838606489072
4	0.003655815703878928
5	0.00913953925969732
6	0.014193166850353484
7	0.02322518211876025
8	0.03580548909975539
9	0.046665412220101614
10	0.05763285933173839
11	0.05526733152334615
12	0.06376172683530013
13	0.06472944275691514
14	0.06677239859143572
15	0.058170479288191176
16	0.06623477863498294
17	0.06300905889626623
18	0.07666460579016693
19	0.07505174592080857
20	0.07860003763339696
21	0.07311631407757856
22	0.07784736969436305
23	0.0809655654417892
24	0.07537431789468026
25	0.07720222574661971
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	930025.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.14568463687092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53158448216375	38.10342800357335
2	13.386015957742986	13.15729837606717
3	3.9683221823191506	5.8507773152926905
4	1.684723858625297	3.3118762982484466
5	0.888981501525226	2.1844802260985374
6	0.5094920591107617	1.5023601637228494
7	0.36024916132205576	1.239328417112169
8	0.2498281407495214	0.9822380014953422
9	0.16949158233599837	0.7496801868680295
>10	1.0112584452997753	9.159722332198587
>50	0.1047633156432537	3.657479982217608
>100	0.11749964699422251	12.198115183952108
>500	0.013616781511327482	4.6132042860950255
>1k	0.0041728846566971315	3.2900112270580832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4374	0.47030993790489506	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3054	0.32837826940136017	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2523	0.27128303002607457	No Hit
GTACATGGGGTGGTATCAACGCAAA	1717	0.18461869304588588	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1706	0.18343592914168974	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1629	0.17515658181231686	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1527	0.16418913470068008	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1328	0.1427918604338593	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1272	0.13677051692158812	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1260	0.13548022902610143	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1205	0.12956640950512083	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1197	0.1287062175747964	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1191	0.12806107362705305	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1163	0.12505040187091745	No Hit
GAACTACGACGGTATCTGATCGTCT	1127	0.12117953818445741	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1118	0.1202118222628424	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1057	0.11365285879411843	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1017	0.10935189914249618	No Hit
GTATCAACGCAGAGTACATGGGGTG	1016	0.10924437515120562	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	997	0.10720141931668505	No Hit
GTACATGGGGAATAATTGCAATCCC	976	0.10494341549958333	No Hit
CTTTAATATACGCTATTGGAGCTGG	950	0.10214779172602888	No Hit
TCGTAGTTCCGACCATAAACGATGC	940	0.10107255181312332	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	936	0.10064245584796108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.075239912905567E-4
7	0.0	0.0	0.0	0.0	1.075239912905567E-4
8	0.0	0.0	0.0	0.0	1.075239912905567E-4
9	0.0	0.0	0.0	0.0	1.075239912905567E-4
10	0.0	0.0	0.0	1.075239912905567E-4	1.075239912905567E-4
11	0.0	0.0	0.0	1.075239912905567E-4	1.075239912905567E-4
12	0.0	0.0	0.0	1.075239912905567E-4	2.150479825811134E-4
13	0.0	0.0	0.0	1.075239912905567E-4	3.225719738716701E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGC	35	0.0021721085	16.282578	5
CGCATCG	245	0.0	15.124419	13
GTATTAC	45	6.779639E-4	14.771753	1
CGGACAT	65	3.3753386E-6	14.61257	5
GCATCGC	245	0.0	14.349581	14
CGAACGA	100	6.566552E-10	14.251857	16
TTAGACC	60	2.5720386E-5	14.246489	4
CTAAACC	40	0.0052893967	14.244957	3
CGCCAGT	255	0.0	14.158708	18
GCTCGTA	95	4.8821676E-9	14.001824	9
ATCGCCA	270	0.0	13.7240095	16
AGACCGT	50	0.0014997162	13.29887	6
GCTTAGG	50	0.0015033692	13.294578	1
CCGCATC	280	0.0	13.23458	12
CGCCCCT	180	0.0	13.191903	5
TTTTCGG	140	3.6379788E-12	12.894538	16
TCGCCAG	275	0.0	12.783484	17
ATCACGA	90	5.385082E-7	12.669	12
CCGACCA	225	0.0	12.668317	9
GGACCGT	60	4.092208E-4	12.66559	6
>>END_MODULE