FastQCFastQC Report
Thu 2 Feb 2017
SRR4064089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930025
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13670.14698529609419103No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13340.14343700438160265No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13260.14257681245127818No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.1405338566167576No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11870.1276309776618908No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11530.12397516195801189No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11520.12386763796672132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.11902905835864627No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11040.1187064863847746No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10680.11483562269831457No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10580.11376038278540901No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG10170.10935189914249618No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9830.10569608343861724No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9790.105265987473455No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9680.10408322356925889No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9440.10150264777828552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA655.934926E-727.0736817
GATATAC4200.022.0009141
GTCCTAA3350.021.0157991
CGCTTCG3150.020.95550532
GACGCTT856.1172723E-620.70340216
CGCTTAC856.1172723E-620.70340218
CGCGGGA3300.020.66641444
CTCGACG755.471408E-520.53308313
AACGTAT1301.6207196E-920.3107231
GTAAACG3250.020.30853727
GCATATA654.9296045E-420.3085371
AACGCTT3350.019.7044330
TAAACGC3400.019.4125728
ACGTATG1152.0169864E-719.13328732
AAACGCT3450.019.1312329
CGGGCCC3450.019.1312337
GATTTCG2650.019.09410741
ACGCTTC3500.018.85995531
ATTTCGT2700.018.7405142
CGACGAG600.00740907218.33409728
Produced by FastQC (version 0.11.5)