##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	930025
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244437515120563	32.0	32.0	32.0	32.0	32.0
2	31.430836805462217	32.0	32.0	32.0	32.0	32.0
3	31.49879089271794	32.0	32.0	32.0	32.0	32.0
4	31.581683288083653	32.0	32.0	32.0	32.0	32.0
5	31.524180532781376	32.0	32.0	32.0	32.0	32.0
6	35.120661272546435	36.0	36.0	36.0	36.0	36.0
7	35.13304481062337	36.0	36.0	36.0	36.0	36.0
8	35.094444772989974	36.0	36.0	36.0	36.0	36.0
9	35.19194107685277	36.0	36.0	36.0	36.0	36.0
10	35.050679282814976	36.0	36.0	36.0	36.0	36.0
11	35.19278836590414	36.0	36.0	36.0	36.0	36.0
12	35.09701459638182	36.0	36.0	36.0	36.0	36.0
13	35.1386629391683	36.0	36.0	36.0	36.0	36.0
14	35.07581409101906	36.0	36.0	36.0	36.0	36.0
15	35.05651891078197	36.0	36.0	36.0	36.0	36.0
16	35.066793903389694	36.0	36.0	36.0	36.0	36.0
17	35.02940458589823	36.0	36.0	36.0	36.0	36.0
18	35.02644660089783	36.0	36.0	36.0	36.0	36.0
19	35.01330824440203	36.0	36.0	36.0	36.0	36.0
20	35.00050428751915	36.0	36.0	36.0	36.0	36.0
21	35.00058278003279	36.0	36.0	36.0	36.0	36.0
22	34.983627321846186	36.0	36.0	36.0	36.0	36.0
23	34.94393914142093	36.0	36.0	36.0	36.0	36.0
24	34.899296255477005	36.0	36.0	36.0	32.0	36.0
25	34.87283352598048	36.0	36.0	36.0	32.0	36.0
26	34.81240289239537	36.0	36.0	36.0	32.0	36.0
27	34.816129673933496	36.0	36.0	36.0	32.0	36.0
28	34.77524045052552	36.0	36.0	36.0	32.0	36.0
29	34.74569608343862	36.0	36.0	36.0	32.0	36.0
30	34.72377194161447	36.0	36.0	36.0	32.0	36.0
31	34.71445176204941	36.0	36.0	36.0	32.0	36.0
32	34.679774199618286	36.0	36.0	36.0	32.0	36.0
33	34.631515281847264	36.0	36.0	36.0	32.0	36.0
34	34.6153888336335	36.0	36.0	36.0	32.0	36.0
35	34.56477836617295	36.0	36.0	36.0	32.0	36.0
36	34.52407085831026	36.0	36.0	36.0	32.0	36.0
37	34.51080884922448	36.0	36.0	36.0	32.0	36.0
38	34.44409666406817	36.0	36.0	36.0	32.0	36.0
39	34.431128195478614	36.0	36.0	36.0	32.0	36.0
40	34.39647321308567	36.0	36.0	36.0	32.0	36.0
41	34.369013736189885	36.0	36.0	36.0	32.0	36.0
42	34.28225262761754	36.0	36.0	36.0	32.0	36.0
43	34.28713959302169	36.0	36.0	36.0	32.0	36.0
44	34.19133571678181	36.0	36.0	36.0	32.0	36.0
45	34.147469154054995	36.0	36.0	36.0	32.0	36.0
46	34.07765812746969	36.0	36.0	36.0	32.0	36.0
47	34.02395957097927	36.0	36.0	36.0	32.0	36.0
48	33.95990752936749	36.0	36.0	36.0	32.0	36.0
49	33.91306470256176	36.0	36.0	36.0	32.0	36.0
50	33.32804064406871	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	1.0
20	9.0
21	30.0
22	118.0
23	327.0
24	772.0
25	1820.0
26	3794.0
27	6980.0
28	11588.0
29	17272.0
30	24661.0
31	34783.0
32	49723.0
33	79339.0
34	161031.0
35	537775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19537436501826	18.37543604588644	12.04648001823758	25.382709570857724
2	15.509690599715062	21.040724711701298	38.04639660224188	25.403188086341764
3	17.99215502428975	25.61218981916557	29.188234256824032	27.207420899720653
4	11.639687105185345	16.14064138060805	36.99147872369022	35.22819279051638
5	13.620816644713852	37.80898362947232	33.342652079245184	15.22754764656864
6	33.05943388618586	36.83653665224053	17.017176957608665	13.086852503964947
7	29.20072041074165	31.34915728071826	21.06620789763716	18.383914410902932
8	27.989062647845646	33.33892458689238	19.4591752470904	19.212837518171575
9	27.854281920184082	13.48924479712693	18.850663698972596	39.80580958371639
10	16.049526082912642	26.918598087127627	31.375407923528115	25.65646790643162
11	37.17921561248353	21.07846563264428	22.95658718851644	18.785731566355743
12	24.878067662769993	24.093786827006614	29.242901258462144	21.785244251761245
13	29.703081015636258	19.601878673316	25.84175790145997	24.85328240958777
14	23.49995645268988	20.36035667935094	25.575926616868617	30.563760251090567
15	24.652222581848797	28.339087955938698	22.8673397299073	24.141349732305205
16	24.805111298921094	26.081323157186205	25.10177222376341	24.01179332012929
17	23.106798204349346	26.43735383457434	25.868551920647292	24.58729604042902
18	24.086767933197205	24.90621179521753	28.041732390989022	22.965287880596243
19	25.376979518808117	24.580871031285216	26.269107033796978	23.77304241610969
20	25.708073886344746	24.206605643086245	25.93882086787101	24.146499602698
21	27.11195935593129	23.855917851670654	25.00577941453187	24.026343377866187
22	25.65460676283623	24.606354244621645	25.845139480271477	23.89389951227065
23	23.774460521774287	24.413479724607775	26.584295589659995	25.22776416395794
24	24.468776007449286	25.12833029756285	26.411095651500716	23.99179804348714
25	24.47199038281638	24.6658609336324	26.576394459366753	24.285754224184465
26	23.686310184348844	25.935830602080053	27.07241907318038	23.305440140390722
27	24.557300403451272	25.14699304068062	26.171428079864427	24.12427847600368
28	23.475729742168163	25.39661887383251	26.859006479667435	24.268644904331893
29	23.596287303843663	24.79446192357403	26.569196923004135	25.04005384957817
30	23.325494547796698	25.606161780060237	26.94864789014087	24.11969578200219
31	24.540866855420507	25.100891544681005	25.64586180681084	24.712379793087653
32	24.08899460101141	25.548689285572195	25.49428003987144	24.868036073544957
33	23.556502767260838	25.055673180419813	26.58763996253687	24.80018408978248
34	24.47843749529567	25.282098594184475	26.512644167596783	23.726819742923073
35	25.100188926153205	25.14674841638593	26.50353282229006	23.2495298351708
36	23.464151527729687	26.112166135092295	25.95807050332171	24.465611833856304
37	24.64545127071942	25.904718893315483	25.632026323005206	23.817803512959888
38	23.989534842458085	25.586514442283022	25.995786838548167	24.428163876710723
39	24.902579388121136	24.9934407755799	25.589900084732275	24.514079751566687
40	25.3305376344086	25.524193548387096	26.02709677419355	23.118172043010755
41	23.77806906147747	25.688426562636092	26.9142554218733	23.619248954013138
42	25.40657946377209	26.426778062719418	25.744853577630465	22.421788895878024
43	24.085929704435195	25.226017616901213	26.30911726249871	24.378935416164882
44	23.599107110215545	26.15014397632724	25.533701643201066	24.717047270256145
45	23.823210313018475	26.34684180475673	25.524591697499698	24.305356184725095
46	23.72684164686401	25.59628391092569	25.504349415597684	25.172525026612618
47	24.305832586916758	25.535833909498827	25.99798494406983	24.16034855951458
48	25.099945161879976	25.99498930119031	24.650702680616337	24.25436285631337
49	24.044214102922517	26.48814003999914	25.111072880153113	24.35657297692523
50	24.194523057866167	26.84772604106631	24.5719391661147	24.385811734952817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	46.0
2	40.0
3	102.0
4	164.0
5	194.0
6	224.0
7	234.0
8	244.0
9	335.0
10	426.0
11	657.5
12	889.0
13	1561.0
14	2233.0
15	3209.5
16	4186.0
17	4608.0
18	5030.0
19	5074.0
20	5118.0
21	5412.0
22	5706.0
23	6239.5
24	6773.0
25	6853.0
26	6933.0
27	8780.0
28	10627.0
29	12601.0
30	14575.0
31	16896.5
32	19218.0
33	22628.5
34	26039.0
35	30372.0
36	34705.0
37	38459.0
38	42213.0
39	43431.0
40	44649.0
41	46772.5
42	48896.0
43	48262.5
44	47629.0
45	52471.5
46	57314.0
47	60734.0
48	64154.0
49	66197.0
50	68240.0
51	64286.5
52	60333.0
53	59396.5
54	58460.0
55	59730.5
56	61001.0
57	59667.0
58	58333.0
59	53128.5
60	47924.0
61	41783.5
62	35643.0
63	31225.5
64	26808.0
65	22924.5
66	19041.0
67	16801.5
68	14562.0
69	13461.0
70	12360.0
71	9516.5
72	6673.0
73	5743.0
74	4813.0
75	3692.0
76	2571.0
77	2329.0
78	2087.0
79	1706.5
80	1326.0
81	1050.0
82	774.0
83	657.0
84	540.0
85	387.0
86	234.0
87	167.5
88	101.0
89	75.5
90	50.0
91	48.0
92	46.0
93	35.0
94	24.0
95	21.0
96	18.0
97	17.0
98	16.0
99	13.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008279347329372867
2	0.0
3	3.225719738716701E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	1.075239912905567E-4
9	0.001075239912905567
10	0.001075239912905567
11	0.0
12	1.075239912905567E-4
13	7.526679390338969E-4
14	2.150479825811134E-4
15	0.005161151581946722
16	7.526679390338969E-4
17	0.0
18	4.300959651622268E-4
19	1.075239912905567E-4
20	2.150479825811134E-4
21	0.0
22	1.075239912905567E-4
23	6.451439477433402E-4
24	3.225719738716701E-4
25	0.0024730517996828043
26	0.005268675573237278
27	0.005268675573237278
28	0.004193435660331712
29	0.002903147764845031
30	0.003655815703878928
31	0.007096583425176742
32	0.003870863686460041
33	0.0047310556167844955
34	0.0033332437300072574
35	0.0034407677212978144
36	0.008924491277116206
37	0.004300959651622268
38	0.00881696728582565
39	0.003978387677750598
40	0.0026880997822639175
41	0.0045160076342033815
42	0.001075239912905567
43	9.677159216150103E-4
44	0.0011827639041961239
45	0.0017203838606489072
46	0.0016128598693583504
47	0.002795623773554474
48	0.0016128598693583504
49	5.376199564527835E-4
50	0.001827907851939464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	930025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.281213801165265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96384303243896	42.86253246205474
2	12.81437904576475	13.911600974246571
3	3.668638513690814	5.974144545625207
4	1.557127126401428	3.3809100185516
5	0.792227744977917	2.1501541802180673
6	0.4998260153593158	1.627869768186212
7	0.29663624733267596	1.1271242893848226
8	0.2256873187931457	0.9800465282897989
9	0.16190369429729584	0.7909496140815014
>10	0.8260064322120384	8.192445430843668
>50	0.0877870270232692	3.325092176200048
>100	0.09356841623693606	10.464595832826012
>500	0.009975310864106132	3.6769196392554666
>1k	0.0023940746073854717	1.5356145402362829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1367	0.14698529609419103	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1334	0.14343700438160265	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1326	0.14257681245127818	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1307	0.1405338566167576	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1187	0.1276309776618908	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1153	0.12397516195801189	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1152	0.12386763796672132	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.11902905835864627	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1104	0.1187064863847746	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1068	0.11483562269831457	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1058	0.11376038278540901	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1017	0.10935189914249618	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	983	0.10569608343861724	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	979	0.105265987473455	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	968	0.10408322356925889	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	944	0.10150264777828552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.075239912905567E-4	0.0
11	0.0	0.0	0.0	1.075239912905567E-4	0.0
12	0.0	0.0	0.0	1.075239912905567E-4	3.225719738716701E-4
13	0.0	0.0	0.0	1.075239912905567E-4	3.225719738716701E-4
14	0.0	0.0	0.0	2.150479825811134E-4	3.225719738716701E-4
15	0.0	0.0	1.075239912905567E-4	3.225719738716701E-4	3.225719738716701E-4
16	0.0	0.0	1.075239912905567E-4	4.300959651622268E-4	3.225719738716701E-4
17	0.0	0.0	1.075239912905567E-4	4.300959651622268E-4	3.225719738716701E-4
18	0.0	0.0	1.075239912905567E-4	4.300959651622268E-4	3.225719738716701E-4
19	0.0	0.0	1.075239912905567E-4	6.451439477433402E-4	3.225719738716701E-4
20	0.0	0.0	1.075239912905567E-4	7.526679390338969E-4	4.300959651622268E-4
21	0.0	0.0	1.075239912905567E-4	0.001075239912905567	4.300959651622268E-4
22	0.0	0.0	1.075239912905567E-4	0.0012902878954866805	4.300959651622268E-4
23	0.0	0.0	1.075239912905567E-4	0.002150479825811134	4.300959651622268E-4
24	0.0	0.0	1.075239912905567E-4	0.004085911669041155	4.300959651622268E-4
25	0.0	0.0	1.075239912905567E-4	0.005161151581946722	4.300959651622268E-4
26	0.0	0.0	1.075239912905567E-4	0.006558963468723959	4.300959651622268E-4
27	0.0	0.0	1.075239912905567E-4	0.00849439531195398	4.300959651622268E-4
28	0.0	0.0	1.075239912905567E-4	0.012902878954866805	4.300959651622268E-4
29	0.0	0.0	1.075239912905567E-4	0.020859654310368	4.300959651622268E-4
30	0.0	0.0	1.075239912905567E-4	0.0384935888820193	4.300959651622268E-4
31	0.0	0.0	1.075239912905567E-4	0.06999811833015242	4.300959651622268E-4
32	0.0	0.0	1.075239912905567E-4	0.10300798365635333	4.300959651622268E-4
33	0.0	0.0	1.075239912905567E-4	0.13128679336576973	5.376199564527835E-4
34	0.0	0.0	1.075239912905567E-4	0.1644041826832612	5.376199564527835E-4
35	0.0	0.0	1.075239912905567E-4	0.20483320340851052	5.376199564527835E-4
36	0.0	0.0	1.075239912905567E-4	0.2592403430015322	5.376199564527835E-4
37	0.0	0.0	1.075239912905567E-4	0.34160372033009867	5.376199564527835E-4
38	0.0	0.0	1.075239912905567E-4	0.44988037955968924	5.376199564527835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	65	5.934926E-7	27.07368	17
GATATAC	420	0.0	22.000914	1
GTCCTAA	335	0.0	21.015799	1
CGCTTCG	315	0.0	20.955505	32
GACGCTT	85	6.1172723E-6	20.703402	16
CGCTTAC	85	6.1172723E-6	20.703402	18
CGCGGGA	330	0.0	20.666414	44
CTCGACG	75	5.471408E-5	20.533083	13
AACGTAT	130	1.6207196E-9	20.31072	31
GTAAACG	325	0.0	20.308537	27
GCATATA	65	4.9296045E-4	20.308537	1
AACGCTT	335	0.0	19.70443	30
TAAACGC	340	0.0	19.41257	28
ACGTATG	115	2.0169864E-7	19.133287	32
AAACGCT	345	0.0	19.13123	29
CGGGCCC	345	0.0	19.13123	37
GATTTCG	265	0.0	19.094107	41
ACGCTTC	350	0.0	18.859955	31
ATTTCGT	270	0.0	18.74051	42
CGACGAG	60	0.007409072	18.334097	28
>>END_MODULE