FastQCFastQC Report
Thu 2 Feb 2017
SRR4064088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872692
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35230.18812490254670816No Hit
TATCAACGCAGAGTACTTTTTTTTT27240.1454590503937647No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20870.11144384661225658No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT456.7540875E-414.78041716
TATCGAC551.9579635E-413.82028114
GCGTTAT1450.013.7555631
CGTTATT1600.013.0554112
TCTTATA1102.7703209E-912.94751552
AGAACCG2150.012.8114495
TTACGCT550.00307334512.0885464
GGTAAAT550.003073959812.08822251
TCGCGTA1201.0002623E-811.8761699
TAGAACC2650.011.8278764
CGCGGTC1850.011.81197410
AACCGCG1850.011.8110277
CAAGACG4300.011.7070134
GAACCGC2250.011.3983816
ACCGTCG3000.011.0844258
GCCTTAT951.3638495E-510.9975571
CGTCGTA2950.010.95023110
CGGACAT2000.010.9228655
CGACAAT700.001489426510.85908317
TAATACT1855.456968E-1210.7816764
Produced by FastQC (version 0.11.5)