##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1872692
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176056180087276	32.0	32.0	32.0	32.0	32.0
2	30.676863039944635	32.0	32.0	32.0	32.0	32.0
3	30.682378629267387	32.0	32.0	32.0	32.0	32.0
4	30.72484476892089	32.0	32.0	32.0	32.0	32.0
5	30.539952111719387	32.0	32.0	32.0	32.0	32.0
6	34.223769311771505	36.0	36.0	36.0	32.0	36.0
7	34.15168271130543	36.0	36.0	36.0	32.0	36.0
8	34.080437680088345	36.0	36.0	36.0	32.0	36.0
9	34.304698263248845	36.0	36.0	36.0	32.0	36.0
10	33.85101073748379	36.0	36.0	36.0	32.0	36.0
11	34.278603208642956	36.0	36.0	36.0	32.0	36.0
12	34.0146046440098	36.0	36.0	36.0	32.0	36.0
13	34.14436383559069	36.0	36.0	36.0	32.0	36.0
14	34.00175629521566	36.0	36.0	36.0	32.0	36.0
15	33.902465541583986	36.0	36.0	36.0	32.0	36.0
16	33.91714227433022	36.0	36.0	36.0	32.0	36.0
17	33.82448849036574	36.0	36.0	36.0	32.0	36.0
18	33.82317487339082	36.0	36.0	36.0	32.0	36.0
19	33.852825237679234	36.0	36.0	36.0	32.0	36.0
20	33.84011946438603	36.0	36.0	36.0	32.0	36.0
21	33.826706687485185	36.0	36.0	36.0	32.0	36.0
22	33.791288690291836	36.0	36.0	36.0	32.0	36.0
23	33.72160771765992	36.0	36.0	36.0	32.0	36.0
24	33.70244492954527	36.0	36.0	36.0	27.0	36.0
25	33.253501376627874	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	14.0
5	24.0
6	77.0
7	30.0
8	95.0
9	120.0
10	174.0
11	59.0
12	109.0
13	99.0
14	158.0
15	316.0
16	480.0
17	723.0
18	962.0
19	1363.0
20	2119.0
21	3256.0
22	5158.0
23	8178.0
24	12249.0
25	17621.0
26	25299.0
27	32657.0
28	44356.0
29	59719.0
30	78650.0
31	105550.0
32	147652.0
33	202290.0
34	401391.0
35	721740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81050083174588	16.956664811219863	11.088235576928215	26.144598780106048
2	17.062537181282313	19.46510219579169	36.941399628961506	26.53096099396448
3	18.739677133579296	23.440576334343124	28.58047603866793	29.239270493409652
4	12.625799816086355	15.040440709210316	35.571323142253256	36.76243633245008
5	14.743210096751817	36.4835572113133	33.51926785411982	15.253964837815076
6	34.70316147462494	35.2079015236196	16.678691916308537	13.41024508544692
7	30.869874306920142	30.272093764804975	20.531828128480086	18.326203799794794
8	28.786221219868402	32.276054225394994	19.111203939236276	19.826520615500325
9	27.618637018564257	13.897521293412893	18.15107518178802	40.33276650623483
10	15.764198999169714	26.472741105957002	31.568469678561012	26.194590216312275
11	37.50372655476139	20.867477835681107	22.29799401827855	19.330801591278952
12	24.801916688796144	23.475163246081745	28.635561437751694	23.087358627370413
13	29.481994437631748	19.19765108829525	25.256678448628243	26.06367602544476
14	23.83423814307393	19.205922585333532	25.038792827067347	31.921046444525185
15	25.395429453162897	26.808578163702773	22.042106477969007	25.75388590516532
16	26.147038754619974	25.54468544632547	23.251436152846953	25.056839646207603
17	24.416479836201866	25.667674019487364	24.824532532010522	25.091313612300247
18	25.169510002800227	24.80035013541562	25.6394567129595	24.390683148824653
19	25.876135949495065	24.786436685544384	25.12768911294675	24.209738252013796
20	25.997240368007933	24.087301417651386	24.638319339227387	25.27713887511329
21	27.01814048657138	23.95391748902791	24.282238546837316	24.745703477563392
22	26.09470532374762	24.032562069240086	24.609714218837926	25.26301838817437
23	24.773311856723794	23.929834762286745	25.128488234448476	26.168365146540985
24	25.07711332184738	24.67375133064568	24.799815312792582	25.449320034714358
25	25.143526824005093	24.23477744591458	25.008590500589712	25.613105229490614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	153.0
2	379.0
3	605.0
4	605.0
5	605.0
6	1604.5
7	2604.0
8	2604.0
9	2604.0
10	2860.5
11	3117.0
12	3117.0
13	3117.0
14	3787.5
15	4458.0
16	4458.0
17	4458.0
18	7541.5
19	10625.0
20	10625.0
21	10625.0
22	17112.5
23	23600.0
24	23600.0
25	23600.0
26	35516.5
27	47433.0
28	47433.0
29	47433.0
30	59291.0
31	71149.0
32	71149.0
33	71149.0
34	90762.5
35	110376.0
36	110376.0
37	110376.0
38	130088.5
39	149801.0
40	149801.0
41	149801.0
42	173601.5
43	197402.0
44	197402.0
45	197402.0
46	220601.0
47	243800.0
48	243800.0
49	243800.0
50	250485.5
51	257171.0
52	257171.0
53	257171.0
54	241352.5
55	225534.0
56	225534.0
57	225534.0
58	207188.0
59	188842.0
60	188842.0
61	188842.0
62	165920.0
63	142998.0
64	142998.0
65	142998.0
66	117882.0
67	92766.0
68	92766.0
69	92766.0
70	70112.0
71	47458.0
72	47458.0
73	47458.0
74	36413.0
75	25368.0
76	25368.0
77	25368.0
78	20257.5
79	15147.0
80	15147.0
81	15147.0
82	10543.0
83	5939.0
84	5939.0
85	5939.0
86	4562.0
87	3185.0
88	3185.0
89	3185.0
90	2316.5
91	1448.0
92	1448.0
93	1448.0
94	1012.5
95	577.0
96	577.0
97	577.0
98	856.5
99	1136.0
100	1136.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03876772047939544
2	0.005873897042332642
3	0.0015485728566149693
4	0.003951530737569232
5	0.009024441819583786
6	0.012975972557153017
7	0.02312179472118213
8	0.03492298786986862
9	0.04640378663442787
10	0.055855420966181304
11	0.052865073380993784
12	0.060394341407983804
13	0.06338468899317133
14	0.06471966559370147
15	0.056389411606393366
16	0.0640254777614258
17	0.05980695170375054
18	0.07561307465402746
19	0.07208873642862788
20	0.07625386342228194
21	0.0722489336206915
22	0.07662765687043038
23	0.0805791876079996
24	0.07582667091011228
25	0.0768412531265152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1872692.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.00539976712122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79180513523349	39.9358852489669
2	13.289366730060603	13.822376588973645
3	4.2818767985685335	6.680421439893533
4	1.8407748219445272	3.829209219859061
5	0.973041838043406	2.5301714888790876
6	0.5918246998688235	1.8466848065240817
7	0.40122126606449426	1.4605970635728172
8	0.2895226293930366	1.2045392066570357
9	0.22112662604305985	1.0349800727871643
>10	1.1581184171188514	10.666427168345678
>50	0.07739912345091271	2.7389511752904547
>100	0.070004673218776	7.669117167344633
>500	0.009999795379102455	3.5550592737012963
>1k	0.00391744561243189	3.0255800792046292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3523	0.18812490254670816	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2724	0.1454590503937647	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2087	0.11144384661225658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.339906402120584E-5
2	0.0	0.0	0.0	0.0	5.339906402120584E-5
3	0.0	0.0	0.0	0.0	5.339906402120584E-5
4	0.0	0.0	0.0	0.0	5.339906402120584E-5
5	0.0	0.0	0.0	0.0	5.339906402120584E-5
6	0.0	0.0	0.0	0.0	5.339906402120584E-5
7	0.0	0.0	0.0	0.0	5.339906402120584E-5
8	0.0	0.0	0.0	0.0	5.339906402120584E-5
9	0.0	0.0	0.0	0.0	5.339906402120584E-5
10	0.0	0.0	0.0	0.0	5.339906402120584E-5
11	0.0	0.0	0.0	5.339906402120584E-5	5.339906402120584E-5
12	0.0	0.0	0.0	5.339906402120584E-5	5.339906402120584E-5
13	0.0	0.0	0.0	5.339906402120584E-5	5.339906402120584E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAT	45	6.7540875E-4	14.780417	16
TATCGAC	55	1.9579635E-4	13.820281	14
GCGTTAT	145	0.0	13.755563	1
CGTTATT	160	0.0	13.055411	2
TCTTATA	110	2.7703209E-9	12.9475155	2
AGAACCG	215	0.0	12.811449	5
TTACGCT	55	0.003073345	12.088546	4
GGTAAAT	55	0.0030739598	12.0882225	1
TCGCGTA	120	1.0002623E-8	11.876169	9
TAGAACC	265	0.0	11.827876	4
CGCGGTC	185	0.0	11.811974	10
AACCGCG	185	0.0	11.811027	7
CAAGACG	430	0.0	11.707013	4
GAACCGC	225	0.0	11.398381	6
ACCGTCG	300	0.0	11.084425	8
GCCTTAT	95	1.3638495E-5	10.997557	1
CGTCGTA	295	0.0	10.950231	10
CGGACAT	200	0.0	10.922865	5
CGACAAT	70	0.0014894265	10.859083	17
TAATACT	185	5.456968E-12	10.781676	4
>>END_MODULE