FastQCFastQC Report
Thu 2 Feb 2017
SRR4064088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872692
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19320.10316699168896967No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19270.10289999636886366No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19220.10263300104875761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32200.023.2984621
ACCGTTA550.00448276720.00134735
AACGCAG40400.018.4047226
CGTCTTA2300.017.21717315
CGTCGTA4400.016.4993510
TCAACGC44750.016.4190254
GTATAGG1753.6288839E-916.3430861
CAACGCA45500.016.1483825
TATCAAC46350.016.0899812
CGAATGC4950.015.99979743
ATCAACG46250.015.8865173
GTAAACG4750.015.7480127
ATACCGT4500.015.6434096
TACTAGC1004.9469783E-415.3993932
CGCAATA5150.015.37926736
ACGCAGA48750.015.2974497
ATACGAA5050.015.24733140
ACCGTCG4650.015.1387838
TATACTT2203.0559022E-1014.9990075
CGAGCCG6800.014.88216315
Produced by FastQC (version 0.11.5)