FastQCFastQC Report
Thu 2 Feb 2017
SRR4064087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185099
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT188101.5872091698668211No Hit
TATCAACGCAGAGTACTTTTTTTTT138021.164628440324395No Hit
GTACTTTTTTTTTTTTTTTTTTTTT121181.0225306071475886No Hit
GTACATGGGGTGGTATCAACGCAAA106490.8985747182302913No Hit
GTCCTAAAGTGTGTATTTCTCATTT99230.837314013428414No Hit
CTTTAGGACGTGAAATATGGCGAGG86990.7340315028533482No Hit
GTCCTACAGTGGACATTTCTAAATT85620.7224712872089167No Hit
CTGTAGGACGTGGAATATGGCAAGA80240.6770742359920985No Hit
GAGTACTTTTTTTTTTTTTTTTTTT78510.6624762994484005No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70760.597080918978077No Hit
GTCCTACAGTGTGCATTTCTCATTT66170.5583499775124272No Hit
GTATCAACGCAGAGTACATGGGGTG64530.5445114711935458No Hit
GGTATCAACGCAGAGTACTTTTTTT64010.5401236521168273No Hit
GTACATGGGTGGTATCAACGCAAAA46080.3888282751061304No Hit
CTGTAGGACCTGGAATATGGCGAGA45330.38249968989932487No Hit
TATCAACGCAGAGTACATGGGGTGG44030.37153014220752867No Hit
CTGAAGGACCTGGAATATGGCGAGA41540.3505192393209344No Hit
ATTTAGAAATGTCCACTGTAGGACG41240.34798780523821216No Hit
GTCCTTCAGTGTGCATTTCTCATTT36190.30537533151238844No Hit
GCAGAGTACTTTTTTTTTTTTTTTT35920.3030970408379384No Hit
GTACATGGGAGTGGTATCAACGCAA33680.28419566635361265No Hit
TTTCTAAATTTTCCACCTTTTTCAG31080.2622565709700202No Hit
GAATATGGCAAGAAAACTGAAAATC30120.2541559819053092No Hit
GGTATCAACGCAGAGTACATGGGGT29040.24504281920750925No Hit
TTGTAGAACAGTGTATATCAATGAG27800.23457955833225747No Hit
ACGCAGAGTACATGGGGTGGTATCA27730.23398889037962228No Hit
GATATACACTGTTCTACAAATCCCG27050.22825097312545195No Hit
ATCAACGCAGAGTACTTTTTTTTTT24670.20816826273585584No Hit
CTGTAGGACATGGAATATGGCAAGA24130.20361168138695587No Hit
GGAATATGGCGAGAAAACTGAAAAT23890.20158653412077812No Hit
GTGTATATCAATGAGTTACAATGAA21580.18209449168381714No Hit
GAAATATGGCGAGGAAAACTGAAAA20540.17331885353038015No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20230.17070303831156722No Hit
GTATCAACGCAGAGTACATGGGAGT19660.16589331355439504No Hit
CCATATTCCAGGTCCTTCAGTGTGC19620.16555578901003207No Hit
GTATCAACGCAGAGTACATGGGTGG19610.16547140787394132No Hit
CCATATTTCACGTCCTAAAGTGTGT19030.1605773019806784No Hit
GCCATATTCCACGTCCTACAGTGGA18890.15939596607540804No Hit
TTCCAGGTCCTTCAGTGTGCATTTC18370.15500814699868956No Hit
GTTCTACAGTGTGGTTTTTATCATT18080.15256109405205812No Hit
GTGTATATCAATGAGTTACAATGAG17650.14893270520015628No Hit
ACAGTGGACATTTCTAAATTTTCCA17530.1479201315670674No Hit
GATATACACTGTTCTACAATGCCGG17180.1449667918038915No Hit
ATACACACTTTAGGACGTGAAATAT16640.14041021045499152No Hit
ACCTGGAATATGGCGAGAAAACTGA16290.1374568706918156No Hit
GTCCACTGTAGGACGTGGAATATGG15870.13391286297600452No Hit
GACCTGGAATATGGCGAGAAAACTG15560.1312970477571916No Hit
CTGTAGAACATATTAGATGAGTGAG15540.1311282854850101No Hit
CCTAAAGTGTGTATTTCTCATTTTC15540.1311282854850101No Hit
GTGTATTTCTCATTTTCCGTGATTT15410.13003133071583048No Hit
GAAATACACACTTTAGGACGTGAAA15140.1277530400413805No Hit
CACTTTAGGACGTGAAATATGGCGA15000.12657170413611016No Hit
GAGTACATGGGGTGGTATCAACGCA14690.1239558889172972No Hit
TCCTAAAGTGTGTATTTCTCATTTT14380.12134007369848426No Hit
GTATCAACGCAGAGTACATGGGAAG13970.11788044711876391No Hit
CCATAGGGTCTTCTCGTCTTATTAT13700.11560215644431393No Hit
CCACTGTAGGACGTGGAATATGGCA13680.11543339417213247No Hit
TATCAACGCAGAGTACATGGGTGGT13580.11458958281122505No Hit
ATCAACGCAGAGTACATGGGGTGGT13460.11357700917813618No Hit
ATGTTCTACAGTGTGGTTTTTATCA13450.11349262804204543No Hit
TATCAACGCAGAGTACATGGGAGTG12870.1085985221487825No Hit
GTTCTACAAATCCCGTTTCCAACGA12520.10564518238560659No Hit
GTAGGACGTGGAATATGGCAAGAAA12350.10421070307206402No Hit
CTACAGTGGACATTTCTAAATTTTC12330.10404194079988253No Hit
GTATTAGAGGCACTGCCTGCCCAGT12320.10395755966379179No Hit
AGTGTGTATTTCTCATTTTCCGTGA12130.10235431807806773No Hit
GTACATGGGCAGTGGTATCAACGCA12060.10176365012543255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA350.002168888216.2867288
GTACTAG551.1280939E-515.5451091
TAAGCCT551.1294487E-515.543144
CCACCTT9650.014.66992713
CACCTTT9950.014.323709514
TAGAAAT11850.014.2679194
CGGTGTT602.5636957E-514.252089514
GCCGGCC400.00527446714.25148813
TTCCACC10500.014.20564611
TAGGACC16900.013.9949584
CCAACGT2400.013.85444419
TGCGAGG551.9592003E-413.81845819
GTATTAT551.959902E-413.8178751
AGGGTTT1101.8553692E-1013.8172916
GAAATGT12150.013.7604296
AAATGTC12350.013.5387337
TTAGGGT1205.0931703E-1113.456334
AATGTCC12850.013.4560918
AATCGTA1001.00480975E-813.3013913
GACGTGT500.001498954113.3002667
Produced by FastQC (version 0.11.5)