##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064087_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185099
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.122781303502915	32.0	32.0	32.0	32.0	32.0
2	30.97070118192657	32.0	32.0	32.0	32.0	32.0
3	31.0274128996818	32.0	32.0	32.0	32.0	32.0
4	30.983965896520036	32.0	32.0	32.0	32.0	32.0
5	31.065991111291122	32.0	32.0	32.0	32.0	32.0
6	34.66252861575278	36.0	36.0	36.0	32.0	36.0
7	34.49362542707402	36.0	36.0	36.0	32.0	36.0
8	34.40574669289232	36.0	36.0	36.0	32.0	36.0
9	34.39637616772945	36.0	36.0	36.0	32.0	36.0
10	34.32477539851101	36.0	36.0	36.0	32.0	36.0
11	34.56140204320483	36.0	36.0	36.0	32.0	36.0
12	34.425383027072	36.0	36.0	36.0	32.0	36.0
13	34.46415194004889	36.0	36.0	36.0	32.0	36.0
14	34.43476536559393	36.0	36.0	36.0	32.0	36.0
15	34.46035647654753	36.0	36.0	36.0	32.0	36.0
16	34.4354286013236	36.0	36.0	36.0	32.0	36.0
17	34.366349140451554	36.0	36.0	36.0	32.0	36.0
18	34.397567629371046	36.0	36.0	36.0	32.0	36.0
19	34.259126874632415	36.0	36.0	36.0	32.0	36.0
20	34.14498451184247	36.0	36.0	36.0	32.0	36.0
21	34.10935963999632	36.0	36.0	36.0	32.0	36.0
22	34.126121108869384	36.0	36.0	36.0	32.0	36.0
23	34.1906920856401	36.0	36.0	36.0	32.0	36.0
24	34.15742482273633	36.0	36.0	36.0	32.0	36.0
25	33.89461555532491	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	8.0
5	17.0
6	48.0
7	17.0
8	67.0
9	83.0
10	99.0
11	30.0
12	71.0
13	65.0
14	300.0
15	403.0
16	571.0
17	680.0
18	878.0
19	1115.0
20	1614.0
21	2179.0
22	3164.0
23	4532.0
24	6335.0
25	8400.0
26	11619.0
27	15123.0
28	20334.0
29	27756.0
30	36709.0
31	49677.0
32	70823.0
33	102483.0
34	236037.0
35	583856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88051159151017	20.112407638924648	13.361196065901467	22.645884703663715
2	10.720316348824198	21.512445771003215	50.08780435114652	17.679433529026067
3	18.87956458451997	30.174208383435648	30.259688205387846	20.68653882665654
4	9.512871641377938	17.772544042556962	40.09182758152208	32.62275673454302
5	8.847126614293636	43.4440458377955	35.215758155753655	12.49306939215721
6	26.07262062999768	40.784353440091145	20.613372154355762	12.529653775555417
7	24.841936815238885	34.07302936924117	22.50342037675207	18.58161343876787
8	23.584841156286007	41.59911535425926	19.70742536856722	15.108618120887515
9	28.730674514630934	13.949891558626302	21.87738231219465	35.44205161454811
10	16.44296746968726	29.476115815309484	34.34405350205969	19.736863212943568
11	31.637855704570494	21.619914812422696	30.34442291537387	16.39780656763294
12	27.860666271503863	24.96694311175508	33.97834372746601	13.194046889275047
13	31.122249091474043	24.136224855108683	27.899937179989465	16.841588873427813
14	19.332219859796908	26.454003353930904	30.136487762205284	24.077289024066904
15	18.569619291268776	39.92586776091931	26.137693457281085	15.366819490530832
16	15.452157459093009	28.674198895074277	37.22491900504335	18.64872464078937
17	15.344639065145163	31.708482676224612	33.13617960831001	19.810698650320216
18	15.686681832998087	27.525878186322945	41.76677627634475	15.020663704334227
19	22.881315142154822	24.483359327567033	31.874278543906332	20.761046986371813
20	23.692557394049228	28.004647934185794	33.223186976326275	15.079607695438707
21	21.804442072516807	24.681520863486746	30.178800934284283	23.335236129712165
22	22.363346644497344	31.849345433572672	30.28341886086948	15.503889061060496
23	17.849234807194385	32.321183367591495	34.20287701009841	15.626704815115703
24	22.413200528629154	28.05863899104455	34.8441768458742	14.683983634452098
25	19.027895295951577	30.99482019297188	35.339811892107335	14.637472618969207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	759.0
1	759.0
2	1873.5
3	2988.0
4	2988.0
5	2988.0
6	8520.5
7	14053.0
8	14053.0
9	14053.0
10	12523.5
11	10994.0
12	10994.0
13	10994.0
14	8075.5
15	5157.0
16	5157.0
17	5157.0
18	10678.0
19	16199.0
20	16199.0
21	16199.0
22	29772.5
23	43346.0
24	43346.0
25	43346.0
26	72984.5
27	102623.0
28	102623.0
29	102623.0
30	135218.0
31	167813.0
32	167813.0
33	167813.0
34	164171.0
35	160529.0
36	160529.0
37	160529.0
38	152003.5
39	143478.0
40	143478.0
41	143478.0
42	140573.5
43	137669.0
44	137669.0
45	137669.0
46	147417.5
47	157166.0
48	157166.0
49	157166.0
50	139202.5
51	121239.0
52	121239.0
53	121239.0
54	83374.0
55	45509.0
56	45509.0
57	45509.0
58	35058.0
59	24607.0
60	24607.0
61	24607.0
62	19229.0
63	13851.0
64	13851.0
65	13851.0
66	11086.5
67	8322.0
68	8322.0
69	8322.0
70	6407.0
71	4492.0
72	4492.0
73	4492.0
74	3345.5
75	2199.0
76	2199.0
77	2199.0
78	1528.0
79	857.0
80	857.0
81	857.0
82	584.5
83	312.0
84	312.0
85	312.0
86	227.5
87	143.0
88	143.0
89	143.0
90	105.0
91	67.0
92	67.0
93	67.0
94	65.5
95	64.0
96	64.0
97	64.0
98	363.5
99	663.0
100	663.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040671707595736725
2	0.006581728615077728
3	0.0020251472661777623
4	0.004387819076718485
5	0.011644596780522133
6	0.014682317679788778
7	0.02430176719413315
8	0.0374652244242886
9	0.04961610802135518
10	0.05872927071915511
11	0.055185263003344025
12	0.06556414274250505
13	0.0643828068372347
14	0.06893938818613465
15	0.06151384821014953
16	0.06463595024550692
17	0.06277956525151063
18	0.07568987907339388
19	0.07273653931021797
20	0.07737750179520868
21	0.07400225635157907
22	0.07425539975985129
23	0.08041522269447532
24	0.07628054702602906
25	0.07467730544030499
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1185099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.008420887836298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.7795908425145	14.44459249270727
2	15.657570379856258	7.205119387613033
3	7.164357191029329	4.945216369259982
4	3.8736105321565093	3.5650264591765
5	2.405791500708833	2.7676731708344073
6	1.5740486151726363	2.1729823821484735
7	1.154691476896386	1.8597339247220543
8	0.792994740432196	1.459644539976357
9	0.6223293175293195	1.2886933381699053
>10	3.2934226329483263	14.18869463298369
>50	0.32738502508357825	5.28922944031709
>100	0.28953911160748086	13.670209406887308
>500	0.03453892955723361	5.423902590106421
>1k	0.02535304403669275	11.152913967214946
>5k	0.003306918787394707	5.884566979237612
>10k+	0.0014697416832865364	4.6818009186449405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	18810	1.5872091698668211	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13802	1.164628440324395	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	12118	1.0225306071475886	No Hit
GTACATGGGGTGGTATCAACGCAAA	10649	0.8985747182302913	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	9923	0.837314013428414	No Hit
CTTTAGGACGTGAAATATGGCGAGG	8699	0.7340315028533482	No Hit
GTCCTACAGTGGACATTTCTAAATT	8562	0.7224712872089167	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8024	0.6770742359920985	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7851	0.6624762994484005	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	7076	0.597080918978077	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6617	0.5583499775124272	No Hit
GTATCAACGCAGAGTACATGGGGTG	6453	0.5445114711935458	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6401	0.5401236521168273	No Hit
GTACATGGGTGGTATCAACGCAAAA	4608	0.3888282751061304	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4533	0.38249968989932487	No Hit
TATCAACGCAGAGTACATGGGGTGG	4403	0.37153014220752867	No Hit
CTGAAGGACCTGGAATATGGCGAGA	4154	0.3505192393209344	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4124	0.34798780523821216	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3619	0.30537533151238844	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3592	0.3030970408379384	No Hit
GTACATGGGAGTGGTATCAACGCAA	3368	0.28419566635361265	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3108	0.2622565709700202	No Hit
GAATATGGCAAGAAAACTGAAAATC	3012	0.2541559819053092	No Hit
GGTATCAACGCAGAGTACATGGGGT	2904	0.24504281920750925	No Hit
TTGTAGAACAGTGTATATCAATGAG	2780	0.23457955833225747	No Hit
ACGCAGAGTACATGGGGTGGTATCA	2773	0.23398889037962228	No Hit
GATATACACTGTTCTACAAATCCCG	2705	0.22825097312545195	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2467	0.20816826273585584	No Hit
CTGTAGGACATGGAATATGGCAAGA	2413	0.20361168138695587	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2389	0.20158653412077812	No Hit
GTGTATATCAATGAGTTACAATGAA	2158	0.18209449168381714	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2054	0.17331885353038015	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2023	0.17070303831156722	No Hit
GTATCAACGCAGAGTACATGGGAGT	1966	0.16589331355439504	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1962	0.16555578901003207	No Hit
GTATCAACGCAGAGTACATGGGTGG	1961	0.16547140787394132	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1903	0.1605773019806784	No Hit
GCCATATTCCACGTCCTACAGTGGA	1889	0.15939596607540804	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1837	0.15500814699868956	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1808	0.15256109405205812	No Hit
GTGTATATCAATGAGTTACAATGAG	1765	0.14893270520015628	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1753	0.1479201315670674	No Hit
GATATACACTGTTCTACAATGCCGG	1718	0.1449667918038915	No Hit
ATACACACTTTAGGACGTGAAATAT	1664	0.14041021045499152	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1629	0.1374568706918156	No Hit
GTCCACTGTAGGACGTGGAATATGG	1587	0.13391286297600452	No Hit
GACCTGGAATATGGCGAGAAAACTG	1556	0.1312970477571916	No Hit
CTGTAGAACATATTAGATGAGTGAG	1554	0.1311282854850101	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1554	0.1311282854850101	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1541	0.13003133071583048	No Hit
GAAATACACACTTTAGGACGTGAAA	1514	0.1277530400413805	No Hit
CACTTTAGGACGTGAAATATGGCGA	1500	0.12657170413611016	No Hit
GAGTACATGGGGTGGTATCAACGCA	1469	0.1239558889172972	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1438	0.12134007369848426	No Hit
GTATCAACGCAGAGTACATGGGAAG	1397	0.11788044711876391	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1370	0.11560215644431393	No Hit
CCACTGTAGGACGTGGAATATGGCA	1368	0.11543339417213247	No Hit
TATCAACGCAGAGTACATGGGTGGT	1358	0.11458958281122505	No Hit
ATCAACGCAGAGTACATGGGGTGGT	1346	0.11357700917813618	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1345	0.11349262804204543	No Hit
TATCAACGCAGAGTACATGGGAGTG	1287	0.1085985221487825	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1252	0.10564518238560659	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1235	0.10421070307206402	No Hit
CTACAGTGGACATTTCTAAATTTTC	1233	0.10404194079988253	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1232	0.10395755966379179	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	1213	0.10235431807806773	No Hit
GTACATGGGCAGTGGTATCAACGCA	1206	0.10176365012543255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.43811360907401E-5	0.0
8	0.0	0.0	0.0	8.43811360907401E-5	0.0
9	0.0	0.0	0.0	1.687622721814802E-4	0.0
10	0.0	0.0	0.0	2.531434082722203E-4	0.0
11	0.0	0.0	0.0	2.531434082722203E-4	0.0
12	0.0	0.0	0.0	3.375245443629604E-4	0.0
13	0.0	0.0	0.0	3.375245443629604E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	35	0.0021688882	16.286728	8
GTACTAG	55	1.1280939E-5	15.545109	1
TAAGCCT	55	1.1294487E-5	15.54314	4
CCACCTT	965	0.0	14.669927	13
CACCTTT	995	0.0	14.3237095	14
TAGAAAT	1185	0.0	14.267919	4
CGGTGTT	60	2.5636957E-5	14.2520895	14
GCCGGCC	40	0.005274467	14.251488	13
TTCCACC	1050	0.0	14.205646	11
TAGGACC	1690	0.0	13.994958	4
CCAACGT	240	0.0	13.854444	19
TGCGAGG	55	1.9592003E-4	13.818458	19
GTATTAT	55	1.959902E-4	13.817875	1
AGGGTTT	110	1.8553692E-10	13.817291	6
GAAATGT	1215	0.0	13.760429	6
AAATGTC	1235	0.0	13.538733	7
TTAGGGT	120	5.0931703E-11	13.45633	4
AATGTCC	1285	0.0	13.456091	8
AATCGTA	100	1.00480975E-8	13.30139	13
GACGTGT	50	0.0014989541	13.300266	7
>>END_MODULE