##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185099
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.844813808804158	32.0	32.0	32.0	32.0	32.0
2	31.449100032993023	32.0	32.0	32.0	32.0	32.0
3	31.52689353378916	32.0	32.0	32.0	32.0	32.0
4	31.61981066560684	32.0	32.0	32.0	32.0	32.0
5	31.53410137043403	32.0	32.0	32.0	32.0	32.0
6	35.094348235885775	36.0	36.0	36.0	36.0	36.0
7	35.14979761184509	36.0	36.0	36.0	36.0	36.0
8	35.138636518974366	36.0	36.0	36.0	36.0	36.0
9	35.22264131519814	36.0	36.0	36.0	36.0	36.0
10	35.10113669828428	36.0	36.0	36.0	36.0	36.0
11	35.22028792531257	36.0	36.0	36.0	36.0	36.0
12	35.16945757274287	36.0	36.0	36.0	36.0	36.0
13	35.18959513087092	36.0	36.0	36.0	36.0	36.0
14	35.137632383454886	36.0	36.0	36.0	36.0	36.0
15	35.12443601758165	36.0	36.0	36.0	36.0	36.0
16	35.13151897014511	36.0	36.0	36.0	36.0	36.0
17	35.11727712199571	36.0	36.0	36.0	36.0	36.0
18	35.096467046213014	36.0	36.0	36.0	36.0	36.0
19	35.056448448610624	36.0	36.0	36.0	36.0	36.0
20	35.0254982917039	36.0	36.0	36.0	36.0	36.0
21	35.0026031580484	36.0	36.0	36.0	36.0	36.0
22	34.992398947260945	36.0	36.0	36.0	36.0	36.0
23	35.02567549208969	36.0	36.0	36.0	36.0	36.0
24	34.996641630783586	36.0	36.0	36.0	36.0	36.0
25	34.95973416566886	36.0	36.0	36.0	36.0	36.0
26	34.93353297910132	36.0	36.0	36.0	36.0	36.0
27	34.87154237747226	36.0	36.0	36.0	36.0	36.0
28	34.80912818254002	36.0	36.0	36.0	32.0	36.0
29	34.765366437740646	36.0	36.0	36.0	32.0	36.0
30	34.682764055998696	36.0	36.0	36.0	32.0	36.0
31	34.64615951916253	36.0	36.0	36.0	32.0	36.0
32	34.55872884881347	36.0	36.0	36.0	32.0	36.0
33	34.54336220011999	36.0	36.0	36.0	32.0	36.0
34	34.43567161899554	36.0	36.0	36.0	32.0	36.0
35	34.356286689972734	36.0	36.0	36.0	32.0	36.0
36	34.262362047390134	36.0	36.0	36.0	32.0	36.0
37	34.148707407566796	36.0	36.0	36.0	32.0	36.0
38	34.061465750962576	36.0	36.0	36.0	32.0	36.0
39	33.852387859579665	36.0	36.0	36.0	32.0	36.0
40	33.870359353944266	36.0	36.0	36.0	32.0	36.0
41	33.79325102797319	36.0	36.0	36.0	32.0	36.0
42	33.73561027390961	36.0	36.0	36.0	27.0	36.0
43	33.67050938360424	36.0	36.0	36.0	27.0	36.0
44	33.448940552645816	36.0	36.0	36.0	21.0	36.0
45	33.306514476849614	36.0	36.0	36.0	14.0	36.0
46	33.004374318094946	36.0	36.0	36.0	14.0	36.0
47	32.869738308782644	36.0	36.0	36.0	14.0	36.0
48	32.68231852360014	36.0	36.0	36.0	14.0	36.0
49	32.51804195261324	36.0	36.0	36.0	14.0	36.0
50	32.09114090890297	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	9.0
21	42.0
22	133.0
23	445.0
24	1266.0
25	3054.0
26	6559.0
27	12477.0
28	20926.0
29	30442.0
30	40140.0
31	53245.0
32	73968.0
33	110028.0
34	199646.0
35	632713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02119490093688	21.095154269781823	13.81997667514485	23.06367415413645
2	10.530894595702957	22.223131667192643	49.019993350738424	18.225980386365976
3	18.727122067954102	30.178956268447905	30.834094173120448	20.25982749047755
4	9.460391072813326	18.414410947946124	40.20778010950984	31.917417869730713
5	8.613963896687112	43.56589618251302	35.133182966148816	12.686956954651047
6	26.402733278372992	40.934792679417804	19.898961857130683	12.763512185078522
7	24.40490153556921	34.30062323959706	22.820813131065954	18.47366209376777
8	23.43107779363208	41.7254452589113	19.763614286425497	15.079862661031123
9	27.969109414844155	13.87131398058875	22.20556145292409	35.954015151643006
10	16.364144188083447	29.770203300843562	34.102866362161215	19.762786148911772
11	31.336733333445842	21.54243784058365	30.47131967145333	16.64950915451718
12	27.482644880545642	25.12722587265009	34.12530788618991	13.264821360614363
13	31.1150470555376	24.064099827101593	28.175267849081376	16.64558526827943
14	19.33379236787568	26.45540951956635	30.485378399724578	23.72541971283339
15	18.789619183279438	39.58466944352558	26.274395965678565	15.351315407516422
16	15.648418560262462	28.506924784678983	37.05732302413908	18.78733363091948
17	15.432732145358443	31.759567563188867	33.169915061876736	19.63778522957595
18	15.747052142523236	26.82546084796363	42.35134984617306	15.076137163340078
19	23.139754569027566	23.836405228592717	32.93842961642867	20.085410585951045
20	23.605737628144386	27.69682277027295	34.09819853192461	14.599241069658058
21	21.482846580749793	24.15477525506308	31.46066278007154	22.90171538411559
22	22.253602655645356	31.180037431503553	31.454023211582506	15.112336701268585
23	17.67584288814708	31.712474643318835	35.022091111913674	15.589591356620414
24	22.396451251968198	27.38930413958724	35.514651158473505	14.699593449971058
25	19.086390792182804	30.345799439700272	35.831251898606006	14.73655786951092
26	14.36731559538577	32.30222019695704	37.82064589082133	15.50981831683586
27	15.866359944776558	31.89736068401457	35.4573486671775	16.778930704031378
28	15.000856511895389	32.55361215959981	33.51467757601226	18.930853752492535
29	17.93103855643727	28.3901008212187	32.22304542861675	21.45581519372728
30	15.313582763875832	30.028117051996293	34.53536824730855	20.12293193681933
31	20.121062576001663	28.046079052697582	33.37912771823289	18.453730653067872
32	16.295081164872236	32.88196263977176	33.81558861725639	17.00736757809962
33	15.475712294227051	32.959029369373624	36.33907264202848	15.226185694370841
34	17.380385803250466	31.96892984321469	35.71447859180126	14.936205761733584
35	15.864104656392636	33.84852568521905	34.226228398754834	16.061141259633487
36	14.737542974901139	32.48303366936879	35.808366962195585	16.971056393534482
37	15.792040774994936	32.83146560453656	30.63499966245865	20.74149395800986
38	17.30877962251286	36.21917072997838	30.762096685721612	15.709952961787149
39	19.809796252462984	31.003126463552018	31.35366167529503	17.833415608689965
40	21.257182107617908	31.400355930444203	33.01959473884511	14.32286722309278
41	20.377013309975688	29.403038350248217	34.70382287344596	15.516125466330141
42	17.465253195372775	35.871292317013385	32.39412255616321	14.269331931450635
43	14.500925251649042	36.26218585533672	33.37437124657092	15.86251764644333
44	14.456817719632667	35.44019353951333	31.65302624883869	18.449962492015317
45	14.97152520118844	35.79268287537666	31.236040007324384	17.99975191611052
46	17.41412423085621	31.166313949053396	30.82287837824154	20.59668344184885
47	21.48235343754615	31.74583887095753	29.163470666413517	17.6083370250828
48	16.29877028464961	33.660768004617445	28.837597072242925	21.202864638490016
49	19.683771653363912	31.798432696843204	29.36005866206281	19.157736987730075
50	16.096705626028935	37.10328661078863	28.079852769557522	18.720154993624906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	124.0
2	53.0
3	189.5
4	326.0
5	409.0
6	492.0
7	631.0
8	770.0
9	1136.0
10	1502.0
11	2356.5
12	3211.0
13	6002.5
14	8794.0
15	13247.5
16	17701.0
17	20833.5
18	23966.0
19	24677.0
20	25388.0
21	25412.5
22	25437.0
23	25949.5
24	26462.0
25	24216.5
26	21971.0
27	28618.5
28	35266.0
29	44688.5
30	54111.0
31	67001.5
32	79892.0
33	101204.0
34	122516.0
35	140152.0
36	157788.0
37	150790.0
38	143792.0
39	123684.5
40	103577.0
41	88313.0
42	73049.0
43	60360.0
44	47671.0
45	43737.5
46	39804.0
47	36851.0
48	33898.0
49	30449.5
50	27001.0
51	25613.5
52	24226.0
53	22375.5
54	20525.0
55	18697.5
56	16870.0
57	15555.5
58	14241.0
59	12129.0
60	10017.0
61	8791.5
62	7566.0
63	6296.5
64	5027.0
65	4433.5
66	3840.0
67	3225.0
68	2610.0
69	2265.5
70	1921.0
71	1571.0
72	1221.0
73	1098.0
74	975.0
75	850.0
76	725.0
77	558.0
78	391.0
79	274.0
80	157.0
81	106.0
82	55.0
83	47.5
84	40.0
85	25.0
86	10.0
87	10.5
88	11.0
89	11.0
90	11.0
91	8.5
92	6.0
93	4.5
94	3.0
95	5.5
96	8.0
97	8.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00818497020080179
2	4.2190568045370047E-4
3	4.2190568045370047E-4
4	0.0
5	0.0
6	1.687622721814802E-4
7	8.43811360907401E-5
8	1.687622721814802E-4
9	0.0010969547691796213
10	6.750490887259208E-4
11	8.43811360907401E-5
12	1.687622721814802E-4
13	8.438113609074009E-4
14	2.531434082722203E-4
15	0.005653536118079587
16	6.750490887259208E-4
17	8.43811360907401E-5
18	8.43811360907401E-5
19	0.0
20	8.438113609074009E-4
21	0.0
22	8.43811360907401E-5
23	5.906679526351807E-4
24	4.2190568045370047E-4
25	0.001603241585724062
26	0.005822298390261067
27	0.008016207928620308
28	0.005062868165444406
29	0.002615815218812943
30	0.004472200212809225
31	0.007763064520348089
32	0.003544007715811084
33	0.004640962484990706
34	0.003290864307538864
35	0.0033752454436296038
36	0.008860019289527711
37	0.0049784870293536655
38	0.008860019289527711
39	0.004556581348899966
40	0.0030377208992666436
41	0.0040502945323555245
42	0.0021939095383592426
43	0.0013500981774518415
44	0.0015188604496333218
45	0.0013500981774518415
46	0.0012657170413611016
47	0.0020251472661777623
48	0.002362671810540723
49	5.062868165444406E-4
50	0.0015188604496333218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1185099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.314709474763326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.31573965393049	25.171809475608182
2	13.698848044161064	9.949393737252777
3	5.884129056666286	6.41041311614548
4	3.1971317516790303	4.644116428590606
5	1.9244574493660274	3.494305658513566
6	1.2652628780095523	2.756859229447188
7	0.8836477387759041	2.246258763817496
8	0.6154263417476168	1.7879223042944887
9	0.4493680105723381	1.468680187606815
>10	2.4066455782819696	15.720838340041801
>50	0.1878341539819269	4.807567396369057
>100	0.14564312421893227	10.364638830024095
>500	0.01654505874983737	4.114832423910536
>1k	0.009088130862586726	6.475328786533888
>5k	2.330289964765827E-4	0.5870353218440594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6937	0.585351941061464	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	4675	0.39448181122420994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4414	0.37245833470452683	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4092	0.34528760888330845	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	4087	0.34486570320285476	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	3670	0.3096787694530162	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	3421	0.2886678665664219	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2983	0.2517089289586777	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2936	0.2477430155624129	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2749	0.23196374311344453	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2687	0.22673211267581866	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	2262	0.1908701298372541	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	2160	0.1822632539559986	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2085	0.17593466874919308	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1903	0.1605773019806784	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	1890	0.15948034721149879	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1864	0.15728643767313955	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1647	0.13897573114144893	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1573	0.13273152707073416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1534	0.1294406627631953	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1427	0.12041188120148613	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1424	0.1201587377932139	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	1367	0.11534901303604173	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1326	0.11188938645632138	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1325	0.11180500532023063	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1217	0.10269184262243071	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1213	0.10235431807806773	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	1208	0.10193241239761404	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1187	0.10016040853970849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	8.43811360907401E-5	0.0
13	0.0	0.0	0.0	8.43811360907401E-5	0.0
14	0.0	0.0	0.0	1.687622721814802E-4	0.0
15	0.0	0.0	0.0	1.687622721814802E-4	0.0
16	0.0	0.0	0.0	2.531434082722203E-4	0.0
17	0.0	0.0	0.0	2.531434082722203E-4	0.0
18	0.0	0.0	0.0	5.906679526351807E-4	0.0
19	0.0	0.0	0.0	8.438113609074009E-4	0.0
20	0.0	0.0	0.0	9.281924969981411E-4	0.0
21	0.0	0.0	0.0	0.0016876227218148019	0.0
22	0.0	0.0	0.0	0.003290864307538864	0.0
23	0.0	0.0	0.0	0.00919754383389067	0.0
24	0.0	0.0	0.0	0.01603241585724062	0.0
25	0.0	0.0	0.0	0.019660804709142443	0.0
26	0.0	0.0	0.0	0.023711099241497967	0.0
27	0.0	0.0	0.0	0.028773967406942373	0.0
28	0.0	0.0	0.0	0.03636826965510898	0.0
29	0.0	0.0	0.0	0.05391954596198292	0.0
30	0.0	0.0	0.0	0.08885333630354933	0.0
31	0.0	0.0	0.0	0.15813024903404693	0.0
32	0.0	0.0	0.0	0.23120431288862786	0.0
33	0.0	0.0	0.0	0.28141108886261823	0.0
34	0.0	0.0	0.0	0.33735578209077893	0.0
35	0.0	0.0	0.0	0.4057888834603691	0.0
36	0.0	0.0	0.0	0.4938827895391018	0.0
37	0.0	0.0	0.0	0.6181762030007619	0.0
38	0.0	0.0	0.0	0.7612022286745664	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1755	0.0	27.200792	4
ATAGGAC	345	0.0	26.781145	3
TATAGGA	370	0.0	26.755293	2
GTCCTAT	215	0.0	26.603197	1
TAGTACG	105	1.1277734E-10	25.141481	4
ACGCTTA	45	0.0013976115	24.446203	13
GCGCAAG	45	0.0013986425	24.44311	1
GTCCGCG	55	1.5932714E-4	24.000713	38
GACGTCG	75	2.0651933E-6	23.465384	7
CTCTAGG	275	0.0	23.198732	1
ACTGTTC	1230	0.0	22.356503	8
ACTACCC	70	3.2134994E-5	22.001583	12
TCCGGAA	50	0.0025799621	22.000654	37
TAACTGC	50	0.0025812266	21.998798	6
GTACGTG	120	5.984475E-10	21.998796	6
TGTAGGA	6000	0.0	21.962133	2
CTGTAGG	5915	0.0	21.757053	1
CTAGGAC	305	0.0	21.63816	3
TTAGGAC	3770	0.0	21.590332	3
AGGACCT	3560	0.0	21.566236	5
>>END_MODULE