FastQCFastQC Report
Thu 2 Feb 2017
SRR4064086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246896
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25860.20739500327212534No Hit
TATCAACGCAGAGTACTTTTTTTTT18620.1493308182879727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG508.730269E-515.1962125
GTCTTAA653.3823726E-614.6105691
CGCGTAA707.248378E-613.57349310
TCGCGTA707.248378E-613.5734939
CCGATAA655.4430195E-513.1558479
ATAACGA655.44507E-513.15531812
CGTCGTA1750.013.03055410
CGAGCCG1900.013.00197815
CGCATCG1950.012.66757713
AACCGCG1155.305992E-912.3931897
CGAACGA701.0890175E-412.21614416
CGATAAC701.0890175E-412.21614410
TAACGAA701.0898341E-412.21516313
AGAACCG1251.4151738E-912.1569695
GGTTTAC550.003064363712.092758
GGGCTTA550.00306620712.0917786
GTGTACG550.00307543812.08692551
GACTATA550.00307543812.08692551
CGCCAGT2000.011.87728318
GCGTAAC802.8608338E-511.87680611
Produced by FastQC (version 0.11.5)