##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064086_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246896
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226767910074297	32.0	32.0	32.0	32.0	32.0
2	30.815254840820725	32.0	32.0	32.0	32.0	32.0
3	30.822118284123135	32.0	32.0	32.0	32.0	32.0
4	30.85617725937047	32.0	32.0	32.0	32.0	32.0
5	30.704837452361705	32.0	32.0	32.0	32.0	32.0
6	34.38393578935212	36.0	36.0	36.0	32.0	36.0
7	34.33687332383776	36.0	36.0	36.0	32.0	36.0
8	34.268666352286	36.0	36.0	36.0	32.0	36.0
9	34.45661787350348	36.0	36.0	36.0	32.0	36.0
10	34.07714757285291	36.0	36.0	36.0	32.0	36.0
11	34.416327424259926	36.0	36.0	36.0	32.0	36.0
12	34.209199484159065	36.0	36.0	36.0	32.0	36.0
13	34.319420384699285	36.0	36.0	36.0	32.0	36.0
14	34.19955553630776	36.0	36.0	36.0	32.0	36.0
15	34.11223871116757	36.0	36.0	36.0	32.0	36.0
16	34.12057300689071	36.0	36.0	36.0	32.0	36.0
17	34.03912435359484	36.0	36.0	36.0	32.0	36.0
18	34.02950687146322	36.0	36.0	36.0	32.0	36.0
19	34.0600667577729	36.0	36.0	36.0	32.0	36.0
20	34.04271246358959	36.0	36.0	36.0	32.0	36.0
21	34.02052456660379	36.0	36.0	36.0	32.0	36.0
22	33.9945737254751	36.0	36.0	36.0	32.0	36.0
23	33.930235560944936	36.0	36.0	36.0	32.0	36.0
24	33.92533459085601	36.0	36.0	36.0	32.0	36.0
25	33.5058336862096	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	11.0
5	13.0
6	46.0
7	15.0
8	74.0
9	83.0
10	105.0
11	49.0
12	57.0
13	58.0
14	116.0
15	195.0
16	358.0
17	475.0
18	622.0
19	823.0
20	1235.0
21	1833.0
22	2912.0
23	4502.0
24	6663.0
25	10039.0
26	14334.0
27	19068.0
28	26122.0
29	35712.0
30	47335.0
31	64113.0
32	90940.0
33	127870.0
34	264703.0
35	526410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.95749255666443	16.72722692325281	11.03386747773819	26.281413042344575
2	17.10265442436142	19.438749975938865	36.99279128409463	26.46580431560509
3	18.879213136211035	23.412550105303342	28.413295970563183	29.294940787922435
4	12.84349588442546	14.930597049041221	35.33852134460548	36.887385721927835
5	14.853287006291366	36.381165041691126	33.40215016506415	15.363397786953357
6	34.771993175797014	34.935780464074675	16.64422144490547	13.648004915222833
7	30.796825340800982	29.999951869933305	20.622209458841574	18.58101333042414
8	28.681110179587876	32.40155158789055	19.169170413930594	19.748167818590975
9	27.71918225343275	13.716407647257528	17.940500825240733	40.623909274068986
10	15.855371065225695	26.116196810132596	31.741636147122925	26.286795977518786
11	37.63349314931092	20.480410840739033	22.491925615358383	19.39417039459167
12	24.929339879592906	23.013130504146517	28.80685912755416	23.25067048870642
13	29.808054196462173	18.91547691939785	25.203971004700144	26.072497879439837
14	23.970204566739188	18.869799060586775	24.88465986195296	32.275336510721075
15	25.51763099061275	26.620059414616765	21.91664299390607	25.945666600864413
16	26.179932717432475	25.138112236053956	23.200207368981964	25.4817476775316
17	24.376897354476974	25.467703144823666	24.835592704850086	25.31980679584927
18	25.2735848829454	24.53615975511193	25.538602054485025	24.65165330745764
19	25.985206848895086	24.338072299599833	24.982784663020812	24.693936188484265
20	26.221647912419538	23.72465768817118	24.4347239834984	25.61897041591088
21	26.976003210272875	23.7053772070626	24.15064205457464	25.167977528089885
22	26.35568700314217	23.675172859372932	24.362757884514288	25.60638225297061
23	25.021109712715273	23.63003583032607	24.9267982585691	26.42205619838956
24	25.261711504551208	24.305560571876658	24.821158120059426	25.611569803512708
25	25.37380874796743	23.784268811629282	24.790241725644044	26.051680714759247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	150.0
2	275.0
3	400.0
4	400.0
5	400.0
6	1087.0
7	1774.0
8	1774.0
9	1774.0
10	1828.5
11	1883.0
12	1883.0
13	1883.0
14	2106.5
15	2330.0
16	2330.0
17	2330.0
18	4086.5
19	5843.0
20	5843.0
21	5843.0
22	9854.0
23	13865.0
24	13865.0
25	13865.0
26	21388.5
27	28912.0
28	28912.0
29	28912.0
30	36848.0
31	44784.0
32	44784.0
33	44784.0
34	57382.5
35	69981.0
36	69981.0
37	69981.0
38	83204.0
39	96427.0
40	96427.0
41	96427.0
42	113126.5
43	129826.0
44	129826.0
45	129826.0
46	146055.0
47	162284.0
48	162284.0
49	162284.0
50	168771.5
51	175259.0
52	175259.0
53	175259.0
54	165222.0
55	155185.0
56	155185.0
57	155185.0
58	143073.5
59	130962.0
60	130962.0
61	130962.0
62	115166.0
63	99370.0
64	99370.0
65	99370.0
66	81122.0
67	62874.0
68	62874.0
69	62874.0
70	47438.0
71	32002.0
72	32002.0
73	32002.0
74	24159.0
75	16316.0
76	16316.0
77	16316.0
78	12848.0
79	9380.0
80	9380.0
81	9380.0
82	6400.5
83	3421.0
84	3421.0
85	3421.0
86	2587.5
87	1754.0
88	1754.0
89	1754.0
90	1305.0
91	856.0
92	856.0
93	856.0
94	599.0
95	342.0
96	342.0
97	342.0
98	529.0
99	716.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03841539310415624
2	0.005774338838203026
3	0.001764381311673147
4	0.0037693600749380864
5	0.008661508257304539
6	0.01259126663330382
7	0.021974567245383735
8	0.03456583387868756
9	0.04755809626464436
10	0.0561394053714183
11	0.053813630006030976
12	0.06335732891917209
13	0.06119195185484595
14	0.06407912127394746
15	0.05918697309158102
16	0.06480091362872284
17	0.06038996034953998
18	0.07530700234823112
19	0.07330202358496618
20	0.0766703879072513
21	0.07185843887541543
22	0.07546740064929233
23	0.08212393014333193
24	0.07723178196096547
25	0.07522680319770053
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1246896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.71765741969584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2248499264744	39.421706753651314
2	13.075027440156322	13.52419579806255
3	4.342264950071391	6.737153133400345
4	1.9264988073346705	3.985360213487485
5	1.0421353117644903	2.6948398519401913
6	0.687167929898575	2.1323229352977644
7	0.4754125485641295	1.7211056323764913
8	0.35851980811604145	1.4833443691456414
9	0.27516395587286024	1.2807751683672801
>10	1.4243508942148808	13.12481708822644
>50	0.09228855408164224	3.324895535069578
>100	0.06822062771607851	7.21776641660928
>500	0.007008962654998761	2.5491705997442224
>1k	0.001090283079666474	0.8025465046214905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2586	0.20739500327212534	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1862	0.1493308182879727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.019915053059759E-5
7	0.0	0.0	0.0	0.0	8.019915053059759E-5
8	0.0	0.0	0.0	0.0	8.019915053059759E-5
9	0.0	0.0	0.0	0.0	8.019915053059759E-5
10	0.0	0.0	0.0	0.0	8.019915053059759E-5
11	0.0	0.0	0.0	0.0	8.019915053059759E-5
12	0.0	0.0	0.0	0.0	8.019915053059759E-5
13	0.0	0.0	0.0	0.0	8.019915053059759E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	8.730269E-5	15.196212	5
GTCTTAA	65	3.3823726E-6	14.610569	1
CGCGTAA	70	7.248378E-6	13.573493	10
TCGCGTA	70	7.248378E-6	13.573493	9
CCGATAA	65	5.4430195E-5	13.155847	9
ATAACGA	65	5.44507E-5	13.155318	12
CGTCGTA	175	0.0	13.030554	10
CGAGCCG	190	0.0	13.001978	15
CGCATCG	195	0.0	12.667577	13
AACCGCG	115	5.305992E-9	12.393189	7
CGAACGA	70	1.0890175E-4	12.216144	16
CGATAAC	70	1.0890175E-4	12.216144	10
TAACGAA	70	1.0898341E-4	12.215163	13
AGAACCG	125	1.4151738E-9	12.156969	5
GGTTTAC	55	0.0030643637	12.09275	8
GGGCTTA	55	0.003066207	12.091778	6
GTGTACG	55	0.003075438	12.0869255	1
GACTATA	55	0.003075438	12.0869255	1
CGCCAGT	200	0.0	11.877283	18
GCGTAAC	80	2.8608338E-5	11.876806	11
>>END_MODULE