FastQCFastQC Report
Thu 2 Feb 2017
SRR4064086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246896
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG407.033043E-427.4994745
TTTAGCG551.593864E-423.99954241
GTATCAA19600.022.1135961
ACCGTCG2550.021.5682138
CGTCGTA2600.021.15174310
CCGTCGT2600.020.3073049
TTAATAC1152.0210791E-719.1308353
CCGCTAG3350.018.38918536
TATGCGA600.00741647818.33151217
TACCGTC3250.018.2765737
GGTATAT750.001290372617.6010761
GTATAGA2003.6379788E-1217.6010741
CGCTAGA3500.017.60036837
ATACCGT3250.017.5996636
CGCAATA3400.017.47165536
GTCGTAG3150.017.45858211
TCGTTTA3200.017.1892430
GTATTAC902.2144073E-417.1121581
CGTATTG3900.016.92614727
AATAACG3650.016.8757112
Produced by FastQC (version 0.11.5)