##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246896
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23497308516508	32.0	32.0	32.0	32.0	32.0
2	31.318055395157256	32.0	32.0	32.0	32.0	32.0
3	31.418402978275655	32.0	32.0	32.0	32.0	32.0
4	31.52529962402638	32.0	32.0	32.0	32.0	32.0
5	31.44030376230255	32.0	32.0	32.0	32.0	32.0
6	35.049202980842026	36.0	36.0	36.0	36.0	36.0
7	35.07147588892738	36.0	36.0	36.0	36.0	36.0
8	35.004726937932276	36.0	36.0	36.0	36.0	36.0
9	35.1438323645276	36.0	36.0	36.0	36.0	36.0
10	34.970485910613235	36.0	36.0	36.0	36.0	36.0
11	35.143416130936345	36.0	36.0	36.0	36.0	36.0
12	35.03784036519485	36.0	36.0	36.0	36.0	36.0
13	35.08729517136955	36.0	36.0	36.0	36.0	36.0
14	35.02937775083086	36.0	36.0	36.0	36.0	36.0
15	34.99393453824537	36.0	36.0	36.0	36.0	36.0
16	35.00905769206093	36.0	36.0	36.0	36.0	36.0
17	34.966377308131555	36.0	36.0	36.0	36.0	36.0
18	34.96674301625797	36.0	36.0	36.0	36.0	36.0
19	34.95920189013358	36.0	36.0	36.0	36.0	36.0
20	34.96424401072744	36.0	36.0	36.0	36.0	36.0
21	34.96002794138405	36.0	36.0	36.0	36.0	36.0
22	34.938735868909674	36.0	36.0	36.0	36.0	36.0
23	34.88970130628376	36.0	36.0	36.0	32.0	36.0
24	34.86014551333872	36.0	36.0	36.0	32.0	36.0
25	34.832698156061134	36.0	36.0	36.0	32.0	36.0
26	34.76775288396145	36.0	36.0	36.0	32.0	36.0
27	34.76119419743106	36.0	36.0	36.0	32.0	36.0
28	34.750588661764894	36.0	36.0	36.0	32.0	36.0
29	34.70921071204014	36.0	36.0	36.0	32.0	36.0
30	34.69741020903107	36.0	36.0	36.0	32.0	36.0
31	34.69630987668579	36.0	36.0	36.0	32.0	36.0
32	34.65192606279914	36.0	36.0	36.0	32.0	36.0
33	34.625398589778136	36.0	36.0	36.0	32.0	36.0
34	34.615949525862625	36.0	36.0	36.0	32.0	36.0
35	34.575496272343486	36.0	36.0	36.0	32.0	36.0
36	34.561974695564025	36.0	36.0	36.0	32.0	36.0
37	34.54637355481131	36.0	36.0	36.0	32.0	36.0
38	34.50888365990427	36.0	36.0	36.0	32.0	36.0
39	34.50552652346306	36.0	36.0	36.0	32.0	36.0
40	34.48749695243228	36.0	36.0	36.0	32.0	36.0
41	34.46350056460202	36.0	36.0	36.0	32.0	36.0
42	34.400458418344435	36.0	36.0	36.0	32.0	36.0
43	34.39337202140355	36.0	36.0	36.0	32.0	36.0
44	34.335919755937944	36.0	36.0	36.0	32.0	36.0
45	34.310243997895576	36.0	36.0	36.0	32.0	36.0
46	34.29253682744992	36.0	36.0	36.0	32.0	36.0
47	34.25796217166468	36.0	36.0	36.0	32.0	36.0
48	34.20992608846287	36.0	36.0	36.0	32.0	36.0
49	34.196556890069424	36.0	36.0	36.0	32.0	36.0
50	33.57407113343856	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	1.0
19	3.0
20	13.0
21	35.0
22	132.0
23	385.0
24	969.0
25	2285.0
26	4850.0
27	8664.0
28	14284.0
29	21959.0
30	31796.0
31	45900.0
32	66932.0
33	110342.0
34	228952.0
35	709391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93940001555984	17.74456026993926	11.603233560501733	26.712806153999164
2	16.308842494273744	20.258194801778508	36.32892449041133	27.10403821353642
3	18.100313580187507	24.019199769025334	28.892123603525572	28.98836304726159
4	12.512591266633304	15.58822868948172	35.597515751113164	36.30166429277181
5	14.472016912396866	36.73794767165826	33.20790186190348	15.582133554041397
6	34.843030086735446	35.357026854707094	16.31219950356686	13.487743554990598
7	30.658099245003985	30.195990998432904	20.575365668613372	18.570544087949735
8	28.593082342071835	32.69182032823908	18.91745582630789	19.797641503381197
9	27.729951430459582	13.513415972128653	17.820032850204353	40.93659974720742
10	15.648663951636316	26.410193072983418	31.747248746076224	26.193894229304043
11	37.73300879384391	20.493305370540423	22.38825241900882	19.38543341660685
12	24.954847878251275	23.079069946491128	28.678253839935326	23.28782833532227
13	29.95679647506715	18.951646858701938	25.131507295356688	25.960049370874234
14	23.978561111498742	18.825833491727035	24.80573714023577	32.38986825653845
15	25.615741026650763	26.716531751295886	21.800388344531054	25.867338877522293
16	26.198165829246484	25.221572157817278	23.200856534483936	25.379405478452306
17	24.550162964673877	25.49218218680628	24.74079634548511	25.216858503034732
18	25.437567968998113	24.490412962790682	25.47213391376318	24.59988515444802
19	26.007301330664305	24.4136640104708	24.9237306078454	24.655304051019492
20	26.29327871205857	23.63955412229958	24.57283687641803	25.49433028922382
21	27.07210545225905	23.727239481079415	24.07257702326417	25.128078043397363
22	26.348793640507758	23.692990250959987	24.51062321355819	25.447592894974065
23	25.025904491822843	23.504516845137655	24.955970383867676	26.513608279171823
24	25.364004770256948	24.24394073910111	24.82065421812457	25.57140027251737
25	25.51837635559006	23.659166844444588	24.960100218626742	25.862356581338613
26	25.219858087769996	24.442925923163358	25.15328910907447	25.183926879992175
27	25.74655745041598	24.254445097660213	24.481823001340207	25.517174450583603
28	25.3081785823705	23.92901426163058	25.167743787905767	25.59506336809315
29	25.264362598416675	24.065758416896106	24.98566406842109	25.68421491626613
30	25.167842560430138	24.40889182606129	25.030216071982824	25.39304954152575
31	25.77518447224896	24.08036573628489	24.228585178055823	25.915864613410335
32	25.462861597464997	24.404525597439335	24.13464831340733	25.997964491688343
33	25.112304354625913	24.05611007069901	24.684501156929343	26.14708441774573
34	25.918461296624823	23.896916113012765	24.83286121706548	25.35176137329693
35	26.28494160559354	23.805926218214307	24.775557277205408	25.133574898986737
36	25.415524740595302	24.4276333677415	24.420334665546996	25.736507226116203
37	26.11725906808058	24.213976706024642	24.469661850781893	25.199102375112886
38	25.41151314443925	24.15734347341717	24.70185699241499	25.729286389728596
39	25.980047367251792	24.019752127957343	24.306313575056564	25.6938869297343
40	26.13783186177836	24.226962458706502	24.571666424726935	25.063539254788203
41	25.184926161868837	24.466715696928816	24.943838427909075	25.404519713293272
42	26.24211535145583	24.59240427144444	24.51428960970739	24.651190767392343
43	25.55039334146563	23.734662730445006	24.892431595316634	25.822512332772735
44	25.270074538166526	24.319861285546782	24.548512364481528	25.861551811805167
45	25.478334305092954	24.47575105523471	24.356413092208626	25.689501547463706
46	25.287155361436408	24.21953570735416	24.618609880935384	25.874699050274042
47	25.563807262928268	24.23593288848967	24.79909854998075	25.40116129860131
48	26.128454817697268	24.61259365360956	23.98623125524308	25.272720273450094
49	25.38070658886255	24.708795950250703	24.276761740391308	25.633735720495437
50	25.500490023883653	24.7993789288126	24.096262826034305	25.603868221269444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	45.5
2	36.0
3	110.0
4	184.0
5	216.0
6	248.0
7	231.5
8	215.0
9	285.0
10	355.0
11	504.0
12	653.0
13	1178.0
14	1703.0
15	2219.5
16	2736.0
17	2727.0
18	2718.0
19	2654.0
20	2590.0
21	2772.5
22	2955.0
23	3402.5
24	3850.0
25	4476.0
26	5102.0
27	6407.0
28	7712.0
29	9096.5
30	10481.0
31	12644.5
32	14808.0
33	17655.5
34	20503.0
35	24344.0
36	28185.0
37	33684.0
38	39183.0
39	44043.0
40	48903.0
41	54304.0
42	59705.0
43	63067.0
44	66429.0
45	73675.5
46	80922.0
47	86412.5
48	91903.0
49	95369.0
50	98835.0
51	98658.0
52	98481.0
53	98519.0
54	98557.0
55	98083.0
56	97609.0
57	94179.5
58	90750.0
59	83319.0
60	75888.0
61	67238.0
62	58588.0
63	51011.0
64	43434.0
65	36746.5
66	30059.0
67	25417.5
68	20776.0
69	18541.5
70	16307.0
71	12665.0
72	9023.0
73	7702.5
74	6382.0
75	5038.0
76	3694.0
77	3156.0
78	2618.0
79	2118.0
80	1618.0
81	1295.0
82	972.0
83	797.5
84	623.0
85	459.0
86	295.0
87	195.0
88	95.0
89	73.5
90	52.0
91	42.0
92	32.0
93	30.0
94	28.0
95	26.0
96	24.0
97	17.5
98	11.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007779317601467966
2	6.415932042447807E-4
3	4.811949031835855E-4
4	0.0
5	0.0
6	8.019915053059759E-5
7	1.6039830106119517E-4
8	0.0
9	0.001924779612734342
10	8.019915053059758E-4
11	8.019915053059759E-5
12	0.0
13	5.613940537141831E-4
14	2.4059745159179274E-4
15	0.0052129447844888425
16	8.821906558365735E-4
17	0.0
18	3.2079660212239034E-4
19	0.0
20	5.613940537141831E-4
21	0.0
22	3.2079660212239034E-4
23	6.415932042447807E-4
24	2.4059745159179274E-4
25	0.001764381311673147
26	0.005533741386611233
27	0.005854537988733623
28	0.004330754128652269
29	0.0023257753653873297
30	0.0034485634728156963
31	0.007699118450937368
32	0.0026465719675097202
33	0.0048921481823664525
34	0.0022455762148567322
35	0.0024059745159179277
36	0.00793971590252916
37	0.004009957526529879
38	0.00761891930040677
39	0.0034485634728156963
40	0.0023257753653873297
41	0.0037693600749380864
42	8.821906558365735E-4
43	0.0012029872579589638
44	0.0014435847095507564
45	0.0013633855590201589
46	8.019915053059758E-4
47	0.0012831864084895614
48	0.0011227881074283661
49	3.2079660212239034E-4
50	0.0014435847095507564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1246896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75729746900827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25693640858734	42.303810484692725
2	12.805407892544332	14.023790583680723
3	4.136867256639203	6.795710128847792
4	1.7743231312407002	3.886285580139568
5	0.9736686586961807	2.6657732190238526
6	0.6387278682107012	2.0985007128815325
7	0.43487082358720336	1.6668645733429979
8	0.31537505003358507	1.3815268343193907
9	0.23469206356353817	1.156599282434765
>10	1.2899160866281227	12.53169888470567
>50	0.07540136437457325	2.834199546661926
>100	0.05836052674427759	6.472023475824834
>500	0.004863369782709586	1.836051830015614
>1k	5.894993676011619E-4	0.34716486342858527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	8.019915053059759E-5
10	0.0	0.0	0.0	0.0	8.019915053059759E-5
11	0.0	0.0	0.0	0.0	8.019915053059759E-5
12	0.0	0.0	0.0	0.0	8.019915053059759E-5
13	0.0	0.0	0.0	0.0	8.019915053059759E-5
14	0.0	0.0	0.0	0.0	8.019915053059759E-5
15	0.0	0.0	0.0	0.0	1.6039830106119517E-4
16	0.0	0.0	0.0	8.019915053059759E-5	1.6039830106119517E-4
17	0.0	0.0	0.0	8.019915053059759E-5	1.6039830106119517E-4
18	0.0	0.0	0.0	2.4059745159179274E-4	1.6039830106119517E-4
19	0.0	0.0	0.0	2.4059745159179274E-4	1.6039830106119517E-4
20	0.0	0.0	0.0	2.4059745159179274E-4	1.6039830106119517E-4
21	0.0	0.0	0.0	4.811949031835855E-4	1.6039830106119517E-4
22	0.0	0.0	0.0	6.415932042447807E-4	1.6039830106119517E-4
23	0.0	0.0	0.0	9.62389806367171E-4	2.4059745159179274E-4
24	0.0	0.0	0.0	0.001523783860081354	2.4059745159179274E-4
25	0.0	0.0	0.0	0.0017643813116731467	2.4059745159179274E-4
26	0.0	0.0	0.0	0.0025663728169791227	2.4059745159179274E-4
27	0.0	0.0	0.0	0.003047567720162708	2.4059745159179274E-4
28	0.0	0.0	0.0	0.004330754128652269	2.4059745159179274E-4
29	0.0	0.0	0.0	0.009222902311018721	3.2079660212239034E-4
30	0.0	0.0	0.0	0.017724012267262064	3.2079660212239034E-4
31	0.0	0.0	0.0	0.03673121094301369	3.2079660212239034E-4
32	0.0	0.0	0.0	0.057262193478846674	3.2079660212239034E-4
33	0.0	0.0	0.0	0.07707138365990428	3.2079660212239034E-4
34	0.0	0.0	0.0	0.09567758658300292	3.2079660212239034E-4
35	0.0	0.0	0.0	0.11877494193581502	3.2079660212239034E-4
36	0.0	0.0	0.0	0.15406256816927796	3.2079660212239034E-4
37	0.0	0.0	0.0	0.21124456249759402	3.2079660212239034E-4
38	0.0	0.0	0.0	0.28398519202884603	3.2079660212239034E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	40	7.033043E-4	27.499474	5
TTTAGCG	55	1.593864E-4	23.999542	41
GTATCAA	1960	0.0	22.113596	1
ACCGTCG	255	0.0	21.568213	8
CGTCGTA	260	0.0	21.151743	10
CCGTCGT	260	0.0	20.307304	9
TTAATAC	115	2.0210791E-7	19.130835	3
CCGCTAG	335	0.0	18.389185	36
TATGCGA	60	0.007416478	18.331512	17
TACCGTC	325	0.0	18.276573	7
GGTATAT	75	0.0012903726	17.601076	1
GTATAGA	200	3.6379788E-12	17.601074	1
CGCTAGA	350	0.0	17.600368	37
ATACCGT	325	0.0	17.599663	6
CGCAATA	340	0.0	17.471655	36
GTCGTAG	315	0.0	17.458582	11
TCGTTTA	320	0.0	17.18924	30
GTATTAC	90	2.2144073E-4	17.112158	1
CGTATTG	390	0.0	16.926147	27
AATAACG	365	0.0	16.875711	2
>>END_MODULE