FastQCFastQC Report
Thu 2 Feb 2017
SRR4064085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421954
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44940.31604397891914926No Hit
TATCAACGCAGAGTACTTTTTTTTT31290.22004931242501513No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23180.1630151186325296No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17680.12433594898287849No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17180.12081966083291021No Hit
GGTATCAACGCAGAGTACTTTTTTT17140.12053835778091274No Hit
GATTAAGAGGGACGGCCGGGGGCAT16410.11540457708195904No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15700.11041144790900409No Hit
GAATAGGACCGCGGTTCTATTTTGT15700.11041144790900409No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15610.1097785160420098No Hit
GCGCAAGACGGACCAGAGCGAAAGC15520.1091455841750155No Hit
GTACATGGGGTGGTATCAACGCAAA15180.10675450823303706No Hit
GAATAACGCCGCCGCATCGCCAGTC14750.10373050042406436No Hit
GTATCTGATCGTCTTCGAACCTCCG14720.10351952313506627No Hit
GAACTACGACGGTATCTGATCGTCT14430.10148007600808465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1550.015.93946916
GCGTTAT1203.6379788E-1214.2500531
GGGTATA400.00527867914.2500521
CCGGTTT500.00149780513.30192212
CGGTCCA2850.012.99913310
AACCGCG1251.03682396E-1012.9182297
ACGAACG1850.012.84104715
TCCAACG604.0842328E-412.66938918
TAGACAG1501.8189894E-1212.6635945
AACGAAC2000.012.35265414
TAACGAA2000.012.3522213
CGTCTTA853.9316346E-612.29719315
CGTTATT1405.2750693E-1112.2100342
GATAACG2050.012.04840211
CGATAAC2050.012.04797710
AGGGGCG1501.4551915E-1112.0304155
ATCGCCA2400.011.8779716
ACGGTAT2400.011.8733729
GTTATTC1850.011.8079463
CTTTCGT1052.7102215E-711.76484716
Produced by FastQC (version 0.11.5)