##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064085_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421954
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.225791410973912	32.0	32.0	32.0	32.0	32.0
2	30.798816980014823	32.0	32.0	32.0	32.0	32.0
3	30.82046606289655	32.0	32.0	32.0	32.0	32.0
4	30.84918640124786	32.0	32.0	32.0	32.0	32.0
5	30.748582584246748	32.0	32.0	32.0	32.0	32.0
6	34.40116487593832	36.0	36.0	36.0	32.0	36.0
7	34.31766639427155	36.0	36.0	36.0	32.0	36.0
8	34.278856418702716	36.0	36.0	36.0	32.0	36.0
9	34.41341984339859	36.0	36.0	36.0	32.0	36.0
10	34.09118790059313	36.0	36.0	36.0	32.0	36.0
11	34.4357742936832	36.0	36.0	36.0	32.0	36.0
12	34.197927640415934	36.0	36.0	36.0	32.0	36.0
13	34.29867421871594	36.0	36.0	36.0	32.0	36.0
14	34.203337801363475	36.0	36.0	36.0	32.0	36.0
15	34.1174313655716	36.0	36.0	36.0	32.0	36.0
16	34.125736134924196	36.0	36.0	36.0	32.0	36.0
17	34.05125060304342	36.0	36.0	36.0	32.0	36.0
18	34.04659574079049	36.0	36.0	36.0	32.0	36.0
19	34.055238073805484	36.0	36.0	36.0	32.0	36.0
20	34.03902306263072	36.0	36.0	36.0	32.0	36.0
21	34.022805238425434	36.0	36.0	36.0	32.0	36.0
22	33.992571489654374	36.0	36.0	36.0	32.0	36.0
23	33.938051441889115	36.0	36.0	36.0	32.0	36.0
24	33.9320533575629	36.0	36.0	36.0	32.0	36.0
25	33.51110373471997	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	21.0
5	8.0
6	41.0
7	22.0
8	78.0
9	94.0
10	136.0
11	33.0
12	84.0
13	65.0
14	165.0
15	248.0
16	429.0
17	529.0
18	745.0
19	1033.0
20	1434.0
21	2204.0
22	3511.0
23	5336.0
24	7975.0
25	11785.0
26	16550.0
27	22079.0
28	30078.0
29	40365.0
30	52902.0
31	71496.0
32	101290.0
33	142886.0
34	301474.0
35	606854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16201263135974	16.983107297610847	11.04952008009101	25.805359990938403
2	16.827677103275022	19.877216648122804	37.34975602277027	25.94535022583191
3	18.890470477200513	24.158706760868068	28.464862249440543	28.485960512490877
4	12.292820496590817	15.263292999834727	36.24339349952	36.200493004054444
5	14.419906599898724	36.86364147864739	33.57979800821471	15.136653913239185
6	34.02291348257786	35.83676271273465	16.894701934120747	13.245621870566746
7	30.112446962961297	30.579299517180065	20.9582822189459	18.349971300912742
8	28.430195215191173	32.5382336574374	19.430204360736905	19.601366766634516
9	27.645786969250718	13.778813159226017	18.493473086375626	40.08192678514764
10	15.696466354763375	26.517573233828255	31.832430671413004	25.953529739995368
11	37.28003670130165	21.09515670878826	22.52901239302197	19.09579419688813
12	24.84914334531273	23.735249473804952	28.914936902334965	22.500670278547357
13	29.63796717799894	19.373509474272357	25.408163911561733	25.580359436166972
14	23.69754226702556	19.668126880244895	25.319610844284934	31.314720008444606
15	25.009200560970342	27.274192197783563	22.566601154450503	25.150006086795596
16	25.568392933546512	25.73376663589074	24.077566599860383	24.620273830702367
17	23.817813978854037	25.872244603557164	25.30028323628239	25.009658181306406
18	24.728615828115853	24.879017273996666	26.52435419575695	23.868012702130535
19	25.313955943416143	24.891969878246183	25.6756985009501	24.118375677387572
20	25.791353944382823	24.178030708249114	25.104232941269206	24.92638240609886
21	26.982550766333407	24.02144523063194	24.597761171971438	24.39824283106321
22	25.888398110456112	24.28698714592166	25.083047593308898	24.74156715031333
23	24.38231112487507	24.262377004180802	25.686594687425217	25.668717183518915
24	24.701947790542842	24.94264166453186	25.609828192431234	24.74558235249407
25	24.748109590275156	24.623607177645027	25.51539353741688	25.112889694662936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	171.0
2	434.5
3	698.0
4	698.0
5	698.0
6	1935.5
7	3173.0
8	3173.0
9	3173.0
10	3352.0
11	3531.0
12	3531.0
13	3531.0
14	3659.5
15	3788.0
16	3788.0
17	3788.0
18	6583.5
19	9379.0
20	9379.0
21	9379.0
22	15233.5
23	21088.0
24	21088.0
25	21088.0
26	31347.5
27	41607.0
28	41607.0
29	41607.0
30	51798.0
31	61989.0
32	61989.0
33	61989.0
34	75908.0
35	89827.0
36	89827.0
37	89827.0
38	102540.0
39	115253.0
40	115253.0
41	115253.0
42	132012.0
43	148771.0
44	148771.0
45	148771.0
46	167776.5
47	186782.0
48	186782.0
49	186782.0
50	189921.0
51	193060.0
52	193060.0
53	193060.0
54	177710.5
55	162361.0
56	162361.0
57	162361.0
58	149136.5
59	135912.0
60	135912.0
61	135912.0
62	119673.0
63	103434.0
64	103434.0
65	103434.0
66	85840.5
67	68247.0
68	68247.0
69	68247.0
70	51122.5
71	33998.0
72	33998.0
73	33998.0
74	26246.0
75	18494.0
76	18494.0
77	18494.0
78	14893.5
79	11293.0
80	11293.0
81	11293.0
82	7831.5
83	4370.0
84	4370.0
85	4370.0
86	3384.0
87	2398.0
88	2398.0
89	2398.0
90	1735.5
91	1073.0
92	1073.0
93	1073.0
94	727.0
95	381.0
96	381.0
97	381.0
98	628.5
99	876.0
100	876.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04015601067263779
2	0.0070325762999365665
3	0.002531727467977164
4	0.004149220016962574
5	0.008017136981927684
6	0.01202570547289153
7	0.021519683477805893
8	0.03495190421068473
9	0.04817314765456548
10	0.05534637548050078
11	0.052814648012523606
12	0.06350416398842719
13	0.06294155788443226
14	0.0653326338264107
15	0.05942526973446398
16	0.06540295958941006
17	0.06181634567644242
18	0.07665508166930857
19	0.07412335420133141
20	0.0772176877733035
21	0.07257618741534536
22	0.07609247556531365
23	0.08256244576125528
24	0.07468596030532633
25	0.0772176877733035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1421954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05858191399216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92205280120739	42.12355674260491
2	13.504981744083908	14.601203237190571
3	4.054261555002449	6.5750289111554725
4	1.6511699251517482	3.570396186109443
5	0.8037336291846648	2.17243501151547
6	0.49270080355078677	1.5980824048703972
7	0.3072967170351049	1.162841732482016
8	0.20860124590525017	0.9021350031303829
9	0.1386041003816824	0.6743467002688532
>10	0.7521189829581919	7.378055630057754
>50	0.07039363978478087	2.705429123041169
>100	0.0790365622070348	9.14774185767982
>500	0.010860942299216443	3.9087105047666744
>1k	0.0041873512478906765	3.4800369551270687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4494	0.31604397891914926	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3129	0.22004931242501513	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2318	0.1630151186325296	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1768	0.12433594898287849	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1718	0.12081966083291021	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1714	0.12053835778091274	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1641	0.11540457708195904	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1570	0.11041144790900409	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1570	0.11041144790900409	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1561	0.1097785160420098	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1552	0.1091455841750155	No Hit
GTACATGGGGTGGTATCAACGCAAA	1518	0.10675450823303706	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1475	0.10373050042406436	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1472	0.10351952313506627	No Hit
GAACTACGACGGTATCTGATCGTCT	1443	0.10148007600808465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8130305199746265E-4
2	0.0	0.0	0.0	0.0	2.8130305199746265E-4
3	0.0	0.0	0.0	0.0	2.8130305199746265E-4
4	0.0	0.0	0.0	0.0	2.8130305199746265E-4
5	0.0	0.0	0.0	0.0	2.8130305199746265E-4
6	0.0	0.0	0.0	0.0	2.8130305199746265E-4
7	0.0	0.0	0.0	7.032576299936566E-5	2.8130305199746265E-4
8	0.0	0.0	0.0	7.032576299936566E-5	2.8130305199746265E-4
9	0.0	0.0	0.0	7.032576299936566E-5	2.8130305199746265E-4
10	0.0	0.0	0.0	1.4065152599873133E-4	3.516288149968283E-4
11	0.0	0.0	0.0	2.10977288998097E-4	3.516288149968283E-4
12	7.032576299936566E-5	0.0	0.0	2.10977288998097E-4	6.329318669942909E-4
13	7.032576299936566E-5	0.0	0.0	2.10977288998097E-4	8.43909155992388E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	155	0.0	15.939469	16
GCGTTAT	120	3.6379788E-12	14.250053	1
GGGTATA	40	0.005278679	14.250052	1
CCGGTTT	50	0.001497805	13.301922	12
CGGTCCA	285	0.0	12.999133	10
AACCGCG	125	1.03682396E-10	12.918229	7
ACGAACG	185	0.0	12.841047	15
TCCAACG	60	4.0842328E-4	12.669389	18
TAGACAG	150	1.8189894E-12	12.663594	5
AACGAAC	200	0.0	12.352654	14
TAACGAA	200	0.0	12.35222	13
CGTCTTA	85	3.9316346E-6	12.297193	15
CGTTATT	140	5.2750693E-11	12.210034	2
GATAACG	205	0.0	12.048402	11
CGATAAC	205	0.0	12.047977	10
AGGGGCG	150	1.4551915E-11	12.030415	5
ATCGCCA	240	0.0	11.87797	16
ACGGTAT	240	0.0	11.873372	9
GTTATTC	185	0.0	11.807946	3
CTTTCGT	105	2.7102215E-7	11.764847	16
>>END_MODULE