FastQCFastQC Report
Thu 2 Feb 2017
SRR4064085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421954
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16910.11892086523192734No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16480.11589685742295461No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16410.11540457708195904No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15740.11069275096100156No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15340.10787972044102694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACT654.9331225E-420.3074674
TACCGTC3950.020.050417
ATACCGT4100.019.3168566
CGTCGTA4150.019.08412610
ACCGTCG4050.019.0121338
ATACGAA4100.018.77961740
GTATAAG2350.018.725831
AATCACG1303.5861376E-818.61648634
ATAACGC5000.018.4804423
GTATCAA38650.018.2171061
CCTATAC1701.2732926E-1018.1180823
ATCGTTT4650.017.9776629
CATCGTT4750.017.59980428
TAATAGT1254.854537E-717.5998024
CGTTTAT4900.017.51062431
GCATCGT4800.017.41708627
CCGTCGT4450.017.3037859
TGGCGAG3450.017.21659318
TATACTG2055.456968E-1217.170545
TAACGCC4750.017.136654
Produced by FastQC (version 0.11.5)