##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1421954
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264524028203443	32.0	32.0	32.0	32.0	32.0
2	31.41152667385865	32.0	32.0	32.0	32.0	32.0
3	31.482622503962858	32.0	32.0	32.0	32.0	32.0
4	31.571363068003606	32.0	32.0	32.0	32.0	32.0
5	31.51890567486712	32.0	32.0	32.0	32.0	32.0
6	35.10956824201064	36.0	36.0	36.0	36.0	36.0
7	35.12319526510703	36.0	36.0	36.0	36.0	36.0
8	35.07920228080515	36.0	36.0	36.0	36.0	36.0
9	35.17549161224625	36.0	36.0	36.0	36.0	36.0
10	35.03719881233852	36.0	36.0	36.0	36.0	36.0
11	35.18635694262965	36.0	36.0	36.0	36.0	36.0
12	35.08652670902153	36.0	36.0	36.0	36.0	36.0
13	35.13444949695982	36.0	36.0	36.0	36.0	36.0
14	35.06529887746017	36.0	36.0	36.0	36.0	36.0
15	35.04874489610775	36.0	36.0	36.0	36.0	36.0
16	35.05675500051338	36.0	36.0	36.0	36.0	36.0
17	35.01559614446037	36.0	36.0	36.0	36.0	36.0
18	35.01432465466534	36.0	36.0	36.0	36.0	36.0
19	35.01389637076868	36.0	36.0	36.0	36.0	36.0
20	35.006047312360316	36.0	36.0	36.0	36.0	36.0
21	35.004609853764606	36.0	36.0	36.0	36.0	36.0
22	34.99344985843424	36.0	36.0	36.0	36.0	36.0
23	34.94068162542529	36.0	36.0	36.0	36.0	36.0
24	34.90669037113718	36.0	36.0	36.0	32.0	36.0
25	34.87566616079001	36.0	36.0	36.0	32.0	36.0
26	34.8178154848891	36.0	36.0	36.0	32.0	36.0
27	34.81701377119091	36.0	36.0	36.0	32.0	36.0
28	34.79147075081191	36.0	36.0	36.0	32.0	36.0
29	34.76366394412196	36.0	36.0	36.0	32.0	36.0
30	34.73542252421667	36.0	36.0	36.0	32.0	36.0
31	34.73489648750944	36.0	36.0	36.0	32.0	36.0
32	34.70079271200053	36.0	36.0	36.0	32.0	36.0
33	34.668004731517335	36.0	36.0	36.0	32.0	36.0
34	34.64791828708945	36.0	36.0	36.0	32.0	36.0
35	34.60475163050282	36.0	36.0	36.0	32.0	36.0
36	34.580176995880315	36.0	36.0	36.0	32.0	36.0
37	34.573763989552404	36.0	36.0	36.0	32.0	36.0
38	34.52416533868184	36.0	36.0	36.0	32.0	36.0
39	34.51882901978545	36.0	36.0	36.0	32.0	36.0
40	34.49008266090183	36.0	36.0	36.0	32.0	36.0
41	34.47333528370116	36.0	36.0	36.0	32.0	36.0
42	34.39280665900585	36.0	36.0	36.0	32.0	36.0
43	34.393701905968825	36.0	36.0	36.0	32.0	36.0
44	34.316195179309595	36.0	36.0	36.0	32.0	36.0
45	34.27356370177938	36.0	36.0	36.0	32.0	36.0
46	34.24428849315801	36.0	36.0	36.0	32.0	36.0
47	34.201353911589266	36.0	36.0	36.0	32.0	36.0
48	34.15566537314147	36.0	36.0	36.0	32.0	36.0
49	34.1260223607796	36.0	36.0	36.0	32.0	36.0
50	33.51775514538445	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	13.0
21	61.0
22	159.0
23	452.0
24	1179.0
25	2603.0
26	5521.0
27	10153.0
28	16746.0
29	25468.0
30	35997.0
31	50930.0
32	73817.0
33	117627.0
34	245278.0
35	835945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61782135879175	17.83676156368378	11.498415413462444	26.047001664062027
2	16.019829191585604	20.556154110147798	36.87304141372656	26.55097528454004
3	18.104657761987244	24.65749474840518	28.87754764763012	28.360299841977454
4	11.942932049841275	15.819991363996303	36.30764426978651	35.929432316375916
5	13.963531872339049	37.25732337332994	33.43926737433138	15.339877379999633
6	34.11320080649755	36.09744639583221	16.72315009448286	13.066202703187383
7	30.106121874890118	30.67330074897148	20.94032842223707	18.28024895390133
8	28.398196002258864	32.74960564800665	19.31301526843714	19.53918308129734
9	27.719851976252063	13.638537372339362	18.36477642422222	40.276834227186356
10	15.61380930278352	26.765686315878305	31.838896155955947	25.781608225382225
11	37.52301410594154	20.987317451900694	22.438208268340606	19.051460173817155
12	24.684535543067238	23.851314145142837	28.985949586167177	22.478200725622756
13	29.610766630870728	19.457589547867045	25.415538813276704	25.51610500798552
14	23.64151150393685	19.597903720811168	25.430254833510084	31.33032994174189
15	25.08982705522685	27.298704057039213	22.55427424873356	25.05719463900038
16	25.618557208542143	25.734244390402395	24.042468799624174	24.604729601431284
17	23.907735411975352	25.870035176946654	25.314320997725666	24.90790841335233
18	24.79891978944333	24.84034192602386	26.50946414945726	23.85127413507555
19	25.360050578324316	24.857994603197152	25.745928311273296	24.03602650720523
20	25.77452337969491	24.105112807230128	25.215849625054236	24.904514188020723
21	27.00804667380239	23.980663228205696	24.742361567251827	24.268928530740094
22	25.86077036301481	24.227453368711906	25.321371381473227	24.590404886800055
23	24.318890195394474	24.112482629414377	25.799539222360373	25.769087952830773
24	24.696227012201554	24.973522275748092	25.591476493547592	24.73877421850276
25	24.70940863391114	24.50328005356108	25.641804648336624	25.145506664191153
26	24.59847428315431	25.38798738860769	25.873679309707015	24.13985901853098
27	25.144351749873756	24.806206457112236	25.22044850668906	24.82899328632495
28	24.39317879090074	24.71795138678883	25.961061045714313	24.92780877659612
29	24.389698435917634	24.843873073028018	25.906028468549568	24.860400022504784
30	24.291093375942747	25.126238828391145	26.14297127521612	24.43969652044999
31	25.043534402727687	24.859339571154486	24.92249610370612	25.174629922411707
32	24.637074711762562	25.02303227049661	24.882517838581407	25.45737517915942
33	24.24410786724205	24.616990272143674	25.61228106696974	25.526620793644543
34	25.135590931723495	24.642526604797443	25.753837757854853	24.468044705624212
35	25.669235962642063	24.532252166085293	25.636674355800608	24.161837515472037
36	24.307667421546427	25.41276918965299	25.256565343986047	25.022998044814535
37	25.400711297277113	25.028254269436374	25.09872291405427	24.472311519232242
38	24.49064419820726	24.878590844993653	25.615098692192184	25.0156662646069
39	25.20892804773328	24.77324370684035	25.02966099608904	24.98816724933733
40	25.355819203476965	24.976124000478222	25.5496402776508	24.118416518394014
41	24.27004822390181	25.442697106495565	25.94434664946913	24.342908020133496
42	25.622780305640923	25.51911838135492	25.287883369786137	23.57021794321802
43	24.848938773787925	24.588941868151963	25.41661392182511	25.145505436235005
44	24.46949014511222	25.303284118672174	25.055101010314125	25.172124725901483
45	24.59630884825742	25.452954799109108	25.164757187534505	24.785979165098972
46	24.42272447884507	25.146736355593564	25.165583872151952	25.264955293409418
47	24.696854829294914	24.892434627399737	25.60196522181886	24.80874532148649
48	25.422154208390975	25.61470899121126	24.45438709137491	24.508749709022858
49	24.479394859597832	25.742026272353968	24.826312580391306	24.952266287656887
50	24.682246822468223	25.807331829750545	24.720152861905976	24.790268485875252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	36.0
2	30.0
3	116.0
4	202.0
5	231.0
6	260.0
7	246.5
8	233.0
9	342.5
10	452.0
11	721.5
12	991.0
13	1770.0
14	2549.0
15	3488.0
16	4427.0
17	4922.0
18	5417.0
19	5532.0
20	5647.0
21	6204.0
22	6761.0
23	7393.5
24	8026.0
25	8735.0
26	9444.0
27	11478.5
28	13513.0
29	15686.5
30	17860.0
31	20379.0
32	22898.0
33	26593.0
34	30288.0
35	34782.5
36	39277.0
37	45994.0
38	52711.0
39	57347.5
40	61984.0
41	67752.0
42	73520.0
43	75511.5
44	77503.0
45	85416.0
46	93329.0
47	99433.5
48	105538.0
49	108927.5
50	112317.0
51	107204.0
52	102091.0
53	100540.0
54	98989.0
55	99622.5
56	100256.0
57	97591.5
58	94927.0
59	86535.0
60	78143.0
61	68781.5
62	59420.0
63	51443.0
64	43466.0
65	37076.5
66	30687.0
67	26279.5
68	21872.0
69	19988.5
70	18105.0
71	13959.0
72	9813.0
73	8399.5
74	6986.0
75	5496.5
76	4007.0
77	3556.0
78	3105.0
79	2568.0
80	2031.0
81	1621.5
82	1212.0
83	1014.5
84	817.0
85	595.0
86	373.0
87	274.5
88	176.0
89	135.5
90	95.0
91	77.5
92	60.0
93	48.0
94	36.0
95	35.0
96	34.0
97	27.5
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009283000715916267
2	7.032576299936566E-4
3	3.516288149968283E-4
4	0.0
5	0.0
6	2.10977288998097E-4
7	2.8130305199746265E-4
8	7.032576299936566E-5
9	0.0014065152599873133
10	9.845606819911192E-4
11	0.0
12	2.10977288998097E-4
13	3.516288149968283E-4
14	4.21954577996194E-4
15	0.003867916964965111
16	0.0011252122079898506
17	0.0
18	6.32931866994291E-4
19	7.032576299936566E-5
20	0.0010548864449904849
21	0.0
22	7.032576299936566E-5
23	7.032576299936566E-4
24	2.8130305199746265E-4
25	0.0018284698379835071
26	0.004571174594958768
27	0.005626061039949253
28	0.002531727467977164
29	0.002391075941978432
30	0.003234985097970821
31	0.006329318669942911
32	0.002531727467977164
33	0.0040788942539632085
34	0.0021097728899809698
35	0.002391075941978432
36	0.006610621721940373
37	0.0031646593349714553
38	0.007665508166930857
39	0.00372726543896638
40	0.001687818311984776
41	0.0031646593349714553
42	0.001687818311984776
43	9.845606819911192E-4
44	0.0014065152599873133
45	9.142349189917536E-4
46	0.0011252122079898506
47	0.0019691213639822383
48	0.001476841022986679
49	2.10977288998097E-4
50	0.001195537970989216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1421954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.50808030520037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72989615050983	45.276051902436045
2	13.291756874176288	15.28766843434658
3	3.8364609631237645	6.618825154652634
4	1.5548012700709104	3.576545451914617
5	0.7704429486722645	2.215334748140998
6	0.42690959923874	1.4730450909649422
7	0.2726639382164602	1.0976265758699069
8	0.18267110787380283	0.8404051792837258
9	0.13215285965118587	0.6839871538844043
>10	0.6615280177446983	6.7722542979839515
>50	0.062118885275431794	2.5238272900742422
>100	0.06738685806240972	8.212040205143396
>500	0.008130712168850774	3.18224734550743
>1k	0.003079815215473778	2.2401411697970732
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1691	0.11892086523192734	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1648	0.11589685742295461	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1641	0.11540457708195904	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1574	0.11069275096100156	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1534	0.10787972044102694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.4065152599873133E-4
6	0.0	0.0	0.0	0.0	2.10977288998097E-4
7	0.0	0.0	0.0	0.0	2.10977288998097E-4
8	0.0	0.0	0.0	0.0	2.10977288998097E-4
9	0.0	0.0	0.0	0.0	2.10977288998097E-4
10	0.0	0.0	0.0	2.8130305199746265E-4	2.10977288998097E-4
11	0.0	0.0	0.0	3.516288149968283E-4	2.10977288998097E-4
12	0.0	0.0	0.0	3.516288149968283E-4	5.626061039949253E-4
13	0.0	0.0	0.0	3.516288149968283E-4	7.032576299936566E-4
14	0.0	0.0	0.0	4.21954577996194E-4	7.032576299936566E-4
15	0.0	0.0	0.0	4.922803409955596E-4	0.0010548864449904849
16	2.8130305199746265E-4	0.0	0.0	6.329318669942909E-4	0.0010548864449904849
17	2.8130305199746265E-4	0.0	0.0	6.329318669942909E-4	0.0010548864449904849
18	2.8130305199746265E-4	0.0	0.0	9.142349189917536E-4	0.0011955379709892163
19	2.8130305199746265E-4	0.0	0.0	9.845606819911192E-4	0.0012658637339885818
20	2.8130305199746265E-4	0.0	0.0	0.0010548864449904849	0.0015471667859860445
21	2.8130305199746265E-4	0.0	0.0	0.0013361894969879475	0.0017581440749841416
22	2.8130305199746265E-4	0.0	0.0	0.001476841022986679	0.0018987956009828728
23	2.8130305199746265E-4	0.0	0.0	0.0023910759419784326	0.0018987956009828728
24	3.516288149968283E-4	0.0	0.0	0.0035162881499682832	0.0018987956009828728
25	3.516288149968283E-4	0.0	0.0	0.004149220016962574	0.0018987956009828728
26	3.516288149968283E-4	0.0	0.0	0.004711826120957499	0.0020394471269816043
27	3.516288149968283E-4	0.0	0.0	0.005485409513950522	0.0020394471269816043
28	3.516288149968283E-4	0.0	0.0	0.008720394611921343	0.0020394471269816043
29	3.516288149968283E-4	0.0	0.0	0.016315577015852834	0.0020394471269816043
30	3.516288149968283E-4	0.0	0.0	0.02981812351173104	0.0020394471269816043
31	3.516288149968283E-4	0.0	0.0	0.056190284636493164	0.0023207501789790667
32	3.516288149968283E-4	0.0	0.0	0.08804785527520581	0.002461401704977798
33	3.516288149968283E-4	0.0	0.0	0.1169517438679451	0.002461401704977798
34	3.516288149968283E-4	0.0	0.0	0.1500751782406463	0.002742704756975261
35	3.516288149968283E-4	0.0	0.0	0.1875588099193082	0.002742704756975261
36	3.516288149968283E-4	0.0	0.0	0.24114704132482484	0.002742704756975261
37	3.516288149968283E-4	0.0	0.0	0.318294403335129	0.002742704756975261
38	3.516288149968283E-4	0.0	0.0	0.42596314648715783	0.0028130305199746265
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACT	65	4.9331225E-4	20.307467	4
TACCGTC	395	0.0	20.05041	7
ATACCGT	410	0.0	19.316856	6
CGTCGTA	415	0.0	19.084126	10
ACCGTCG	405	0.0	19.012133	8
ATACGAA	410	0.0	18.779617	40
GTATAAG	235	0.0	18.72583	1
AATCACG	130	3.5861376E-8	18.616486	34
ATAACGC	500	0.0	18.480442	3
GTATCAA	3865	0.0	18.217106	1
CCTATAC	170	1.2732926E-10	18.118082	3
ATCGTTT	465	0.0	17.97766	29
CATCGTT	475	0.0	17.599804	28
TAATAGT	125	4.854537E-7	17.599802	4
CGTTTAT	490	0.0	17.510624	31
GCATCGT	480	0.0	17.417086	27
CCGTCGT	445	0.0	17.303785	9
TGGCGAG	345	0.0	17.216593	18
TATACTG	205	5.456968E-12	17.17054	5
TAACGCC	475	0.0	17.13665	4
>>END_MODULE