FastQCFastQC Report
Thu 2 Feb 2017
SRR4064084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356156
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71862.0176551848066575No Hit
TATCAACGCAGAGTACTTTTTTTTT52451.4726692797538157No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48161.3522164444793854No Hit
GTACATGGGGTGGTATCAACGCAAA36841.034378193825178No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31660.8889363088084997No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26620.7474252855490291No Hit
GGTATCAACGCAGAGTACTTTTTTT24810.6966048585451319No Hit
GTATCAACGCAGAGTACATGGGGTG19810.5562169386448634No Hit
GTACATGGGTGGTATCAACGCAAAA17990.5051157358011659No Hit
TATCAACGCAGAGTACATGGGGTGG14470.40628264019137683No Hit
GTACATGGGAGTGGTATCAACGCAA13310.37371264277451455No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13290.37315109109491346No Hit
GGTATCAACGCAGAGTACATGGGGT10740.3015532519457766No Hit
GTACATGGAAGCAGTGGTATCAACG8720.2448365323060681No Hit
ACGCAGAGTACATGGGGTGGTATCA8390.23557092959265044No Hit
GTATCAACGCAGAGTACATGGGAGT7850.22040903424342143No Hit
ATCAACGCAGAGTACTTTTTTTTTT7620.21395118992800907No Hit
TCCATGTACTCTGCGTTGATACCAC7220.20272015633598758No Hit
GTATCAACGCAGAGTACATGGGAAG6850.19233145026336773No Hit
GTATCAACGCAGAGTACATGGGTGG6640.18643515762755647No Hit
GTCCTACAGTGGACATTTCTAAATT6230.17492334819573443No Hit
GTCCTAAAGTGTGTATTTCTCATTT6120.17183481395792854No Hit
GAGTACATGGGGTGGTATCAACGCA5450.15302283269129258No Hit
TATCAACGCAGAGTACATGGGAGTG5290.148530419254484No Hit
GGTATCAACGCAGAGTACATGGGAG4950.13898404070126574No Hit
CTTTAGGACGTGAAATATGGCGAGG4900.13758016150226304No Hit
CCCATGTACTCTGCGTTGATACCAC4860.1364570581430609No Hit
TATCAACGCAGAGTACATGGGTGGT4740.13308774806545445No Hit
CTGTAGGACGTGGAATATGGCAAGA4730.1328069722256539No Hit
GTACATGGGAAGCAGTGGTATCAAC4570.12831455878884532No Hit
GGTATCAACGCAGAGTACATGGGAA4260.1196105077550287No Hit
GGTATCAACGCAGAGTACATGGGTG4130.11596042183762172No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4040.11343343927941688No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3910.10978335336200992No Hit
GGTGGTATCAACGCAAAAAAAAAAA3860.10837947416300721No Hit
GTATCAACGCAGAGTACATGGGCAG3700.10388706072619863No Hit
GTCCTACAGTGTGCATTTCTCATTT3640.10220240568739541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC250.00602983718.996353
AAATCCC350.002162465816.2894518
GTCTATA350.002170601416.2802961
CTAGGAC508.69815E-515.1970793
GTTACAA400.005260516414.25326715
TTACAAT400.005260516414.25326716
AAAGATC400.005265424514.25126510
AGGACTA400.005270336814.2492635
ACAGCGC400.005270336814.2492638
GTCCTAA2050.013.4345541
TTATGCA500.001496172613.2993124
ATATAGA655.446469E-513.149471
TAGGACC1207.3123374E-1012.6660114
CTGTAGG2500.011.7760811
CTAAAGT2100.011.7612954
CTCGAAC657.9814123E-411.69498818
TAATACT658.00026E-411.6917034
TGTAGGA2600.011.6851362
GTAGGAC2800.011.5334973
TAAAGTG2250.011.399415
Produced by FastQC (version 0.11.5)