##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064084_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356156
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.968786149889375	32.0	32.0	32.0	32.0	32.0
2	30.784364155033188	32.0	32.0	32.0	32.0	32.0
3	30.854583384808905	32.0	32.0	32.0	32.0	32.0
4	30.79932108401936	32.0	32.0	32.0	32.0	32.0
5	30.823919855344286	32.0	32.0	32.0	32.0	32.0
6	34.360791338626896	36.0	36.0	36.0	32.0	36.0
7	34.1953806758836	36.0	36.0	36.0	32.0	36.0
8	34.099790541223506	36.0	36.0	36.0	32.0	36.0
9	34.1416149103202	36.0	36.0	36.0	32.0	36.0
10	34.03682375138984	36.0	36.0	36.0	32.0	36.0
11	34.33885151450488	36.0	36.0	36.0	32.0	36.0
12	34.1910230348499	36.0	36.0	36.0	32.0	36.0
13	34.20844798346792	36.0	36.0	36.0	32.0	36.0
14	34.15938521322117	36.0	36.0	36.0	32.0	36.0
15	34.18627230764047	36.0	36.0	36.0	32.0	36.0
16	34.1369596468963	36.0	36.0	36.0	32.0	36.0
17	34.049422163321694	36.0	36.0	36.0	32.0	36.0
18	34.04699064454902	36.0	36.0	36.0	32.0	36.0
19	33.99025427060052	36.0	36.0	36.0	32.0	36.0
20	33.8958293556756	36.0	36.0	36.0	32.0	36.0
21	33.8558524916048	36.0	36.0	36.0	32.0	36.0
22	33.90756017026247	36.0	36.0	36.0	32.0	36.0
23	33.92480542234302	36.0	36.0	36.0	32.0	36.0
24	33.93814508249194	36.0	36.0	36.0	32.0	36.0
25	33.61183863250935	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	7.0
6	19.0
7	11.0
8	23.0
9	17.0
10	33.0
11	10.0
12	20.0
13	18.0
14	103.0
15	115.0
16	202.0
17	243.0
18	312.0
19	395.0
20	617.0
21	913.0
22	1242.0
23	1735.0
24	2347.0
25	3167.0
26	4466.0
27	5663.0
28	7552.0
29	9680.0
30	13065.0
31	16997.0
32	23800.0
33	33272.0
34	71766.0
35	158345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.005850581266344	17.87952240382212	12.729438001533566	20.385189013377975
2	13.533950703948513	22.142227363857426	43.45218372859654	20.871638203597524
3	18.691405098370645	31.900334968398415	28.52004615993688	20.888213773294062
4	10.100295382818379	16.30024596516055	37.35497602120466	36.24448263081641
5	10.92356509041896	42.448332022913625	31.592721554532183	15.035381332135236
6	27.105436082920065	37.856007548399056	21.308782315179357	13.729774053501526
7	26.75303593695586	34.406349349000756	21.419784984890526	17.42082972915286
8	29.43089704915823	31.64894364021414	20.239920906430285	18.680238404197354
9	30.9198679682562	12.84921693939181	20.63066226560854	35.60025282674345
10	16.939272028498547	29.071100273073597	31.33154280962388	22.658084888803984
11	33.39298356013529	24.05046577743817	23.83078064073895	18.725770021687588
12	26.15127004239562	27.102291723442335	30.42061286815215	16.325825366009894
13	30.77422327614788	23.252827898560906	26.794935912204497	19.17801291308672
14	23.41909254633779	21.232640010788973	31.02306985578179	24.325197587091445
15	23.00437432046322	29.98305899011353	28.363689487864562	18.648877201558687
16	19.772587429091615	28.971642264910074	29.720693307709382	21.535076998288925
17	18.355575534515214	28.268198803135448	29.524906582755033	23.8513190795943
18	18.618125823664787	27.51187903753804	34.19280148589829	19.67719365289888
19	21.775047837949472	25.932523904925414	33.07716731902362	19.215260938101487
20	25.02437722169924	23.98212805417785	32.370421367653464	18.623073356469448
21	26.21092147485431	23.738500531068937	29.733397773444302	20.31718022063245
22	23.59212415665139	26.51226701660424	30.510322502690567	19.385286324053805
23	21.249290463264263	27.591003355195493	31.48657078788083	19.67313539365941
24	20.836810677906566	27.470038637161924	32.34984193888303	19.34330874604847
25	21.202805534636436	27.743994964425013	31.948362876122605	19.104836624815942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	258.0
1	258.0
2	682.5
3	1107.0
4	1107.0
5	1107.0
6	3267.0
7	5427.0
8	5427.0
9	5427.0
10	4837.5
11	4248.0
12	4248.0
13	4248.0
14	3016.5
15	1785.0
16	1785.0
17	1785.0
18	3246.5
19	4708.0
20	4708.0
21	4708.0
22	7221.0
23	9734.0
24	9734.0
25	9734.0
26	14999.0
27	20264.0
28	20264.0
29	20264.0
30	24335.0
31	28406.0
32	28406.0
33	28406.0
34	29246.5
35	30087.0
36	30087.0
37	30087.0
38	31524.5
39	32962.0
40	32962.0
41	32962.0
42	36254.5
43	39547.0
44	39547.0
45	39547.0
46	47154.5
47	54762.0
48	54762.0
49	54762.0
50	52783.5
51	50805.0
52	50805.0
53	50805.0
54	39952.0
55	29099.0
56	29099.0
57	29099.0
58	23813.5
59	18528.0
60	18528.0
61	18528.0
62	15016.0
63	11504.0
64	11504.0
65	11504.0
66	9039.0
67	6574.0
68	6574.0
69	6574.0
70	4970.5
71	3367.0
72	3367.0
73	3367.0
74	2499.0
75	1631.0
76	1631.0
77	1631.0
78	1137.0
79	643.0
80	643.0
81	643.0
82	445.0
83	247.0
84	247.0
85	247.0
86	174.0
87	101.0
88	101.0
89	101.0
90	77.5
91	54.0
92	54.0
93	54.0
94	43.5
95	33.0
96	33.0
97	33.0
98	154.0
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034535428295466034
2	0.00617706847561181
3	8.423275194016104E-4
4	0.002246206718404295
5	0.010107930232819327
6	0.015161895349228991
7	0.02470827390244724
8	0.034254652455665495
9	0.05082042700389717
10	0.058401374678511656
11	0.053908961241703066
12	0.06261301227551971
13	0.06682464987252777
14	0.06654387403272723
15	0.060366805557115426
16	0.0671054257123283
17	0.06345533979492132
18	0.0777749076247487
19	0.07496714922674333
20	0.08170576938195621
21	0.07580947674614495
22	0.07945956266355192
23	0.08142499354215568
24	0.07889801098395086
25	0.08086344186255462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	356156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93044697188751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58259886283669	36.00429451711701
2	15.034122052259294	14.71252623693916
3	5.299965378916858	7.779890247777931
4	2.306796586941753	4.514903522891381
5	1.1887743884528128	2.908363108786418
6	0.6963462353359157	2.0443519525306515
7	0.4270449463115741	1.462685007007772
8	0.2585558668855735	1.0121003307131968
9	0.20025768481760575	0.8818828224912738
>10	0.8673587192988554	7.4556775950683285
>50	0.06563515702297872	2.2148478611356803
>100	0.05872619312582304	6.09491649397996
>500	0.006333216905726014	2.151743572036928
>1k	0.006333216905726014	7.259810402119878
>5k	0.0011514939828592755	3.5020063294044315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7186	2.0176551848066575	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5245	1.4726692797538157	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4816	1.3522164444793854	No Hit
GTACATGGGGTGGTATCAACGCAAA	3684	1.034378193825178	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3166	0.8889363088084997	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2662	0.7474252855490291	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2481	0.6966048585451319	No Hit
GTATCAACGCAGAGTACATGGGGTG	1981	0.5562169386448634	No Hit
GTACATGGGTGGTATCAACGCAAAA	1799	0.5051157358011659	No Hit
TATCAACGCAGAGTACATGGGGTGG	1447	0.40628264019137683	No Hit
GTACATGGGAGTGGTATCAACGCAA	1331	0.37371264277451455	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1329	0.37315109109491346	No Hit
GGTATCAACGCAGAGTACATGGGGT	1074	0.3015532519457766	No Hit
GTACATGGAAGCAGTGGTATCAACG	872	0.2448365323060681	No Hit
ACGCAGAGTACATGGGGTGGTATCA	839	0.23557092959265044	No Hit
GTATCAACGCAGAGTACATGGGAGT	785	0.22040903424342143	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	762	0.21395118992800907	No Hit
TCCATGTACTCTGCGTTGATACCAC	722	0.20272015633598758	No Hit
GTATCAACGCAGAGTACATGGGAAG	685	0.19233145026336773	No Hit
GTATCAACGCAGAGTACATGGGTGG	664	0.18643515762755647	No Hit
GTCCTACAGTGGACATTTCTAAATT	623	0.17492334819573443	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	612	0.17183481395792854	No Hit
GAGTACATGGGGTGGTATCAACGCA	545	0.15302283269129258	No Hit
TATCAACGCAGAGTACATGGGAGTG	529	0.148530419254484	No Hit
GGTATCAACGCAGAGTACATGGGAG	495	0.13898404070126574	No Hit
CTTTAGGACGTGAAATATGGCGAGG	490	0.13758016150226304	No Hit
CCCATGTACTCTGCGTTGATACCAC	486	0.1364570581430609	No Hit
TATCAACGCAGAGTACATGGGTGGT	474	0.13308774806545445	No Hit
CTGTAGGACGTGGAATATGGCAAGA	473	0.1328069722256539	No Hit
GTACATGGGAAGCAGTGGTATCAAC	457	0.12831455878884532	No Hit
GGTATCAACGCAGAGTACATGGGAA	426	0.1196105077550287	No Hit
GGTATCAACGCAGAGTACATGGGTG	413	0.11596042183762172	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	404	0.11343343927941688	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	391	0.10978335336200992	No Hit
GGTGGTATCAACGCAAAAAAAAAAA	386	0.10837947416300721	No Hit
GTATCAACGCAGAGTACATGGGCAG	370	0.10388706072619863	No Hit
GTCCTACAGTGTGCATTTCTCATTT	364	0.10220240568739541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGAC	25	0.006029837	18.99635	3
AAATCCC	35	0.0021624658	16.28945	18
GTCTATA	35	0.0021706014	16.280296	1
CTAGGAC	50	8.69815E-5	15.197079	3
GTTACAA	40	0.0052605164	14.253267	15
TTACAAT	40	0.0052605164	14.253267	16
AAAGATC	40	0.0052654245	14.251265	10
AGGACTA	40	0.0052703368	14.249263	5
ACAGCGC	40	0.0052703368	14.249263	8
GTCCTAA	205	0.0	13.434554	1
TTATGCA	50	0.0014961726	13.299312	4
ATATAGA	65	5.446469E-5	13.14947	1
TAGGACC	120	7.3123374E-10	12.666011	4
CTGTAGG	250	0.0	11.776081	1
CTAAAGT	210	0.0	11.761295	4
CTCGAAC	65	7.9814123E-4	11.694988	18
TAATACT	65	8.00026E-4	11.691703	4
TGTAGGA	260	0.0	11.685136	2
GTAGGAC	280	0.0	11.533497	3
TAAAGTG	225	0.0	11.39941	5
>>END_MODULE