FastQCFastQC Report
Thu 2 Feb 2017
SRR4064084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356156
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24750.6949202035063287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.48405754781612553No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA14910.41863677714260045No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12510.35125057559047157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.3175574748144072No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10410.29228764923235884No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.25185592830108156No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA8500.2386594638304563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.23613248127225148No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA6650.18671593346735701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6530.18334662338975055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5800.16284998708431137No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA4780.1342108514246566No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4480.12578757623064052No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA4150.1165219735172228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT4020.1128718875998158No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3930.11034490504161097No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3880.10894102584260829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3710.10416783656599918No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3710.10416783656599918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG300.00573821129.3348329
ACGCCCG554.951604E-627.99749821
CCCGGCT551.5915069E-423.99785424
CACGCCC602.8690047E-421.99803520
ACTGTTC703.2093616E-521.9980358
TAGGACC1407.2759576E-1221.9980354
GTATTAC500.00257823621.9980341
GACGTGA3050.021.6374117
GCGAGGA3150.020.9505120
ACCACGC755.4625107E-520.53149818
TTAGGAC3350.020.356393
CCGCCTG550.004476187820.001029
GTGCCCA550.004476187820.0010211
GCATTAA550.004479827419.9982131
GTAGGAC4200.019.9029853
TGTAGGA4150.019.6127052
CTTTAGG3400.019.4100321
TAGGACG5100.019.410034
CCTGAAC808.972062E-519.248283
AGGACGT5050.019.1666055
Produced by FastQC (version 0.11.5)