FastQCFastQC Report
Thu 2 Feb 2017
SRR4064083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282781
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71840.3147038634017017No Hit
TATCAACGCAGAGTACTTTTTTTTT51520.2256896303237148No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36340.15919179281762025No Hit
GTCCTAAAGTGTGTATTTCTCATTT33490.1467070209538278No Hit
GTCCTACAGTGGACATTTCTAAATT29540.12940356521278215No Hit
CTTTAGGACGTGAAATATGGCGAGG28130.12322688860648481No Hit
CTGTAGGACGTGGAATATGGCAAGA26440.11582363792234122No Hit
GTCCTACAGTGTGCATTTCTCATTT26310.11525415710048402No Hit
GGTATCAACGCAGAGTACTTTTTTT24830.10877083697472512No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24050.10535395204358193No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23090.10114855520525184No Hit
GTACATGGGGTGGTATCAACGCAAA22940.10049146194926276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG2950.012.23991615
CCAACGA2100.012.216639519
GCGTTAT1650.012.0876741
CGTTATT1650.012.0855552
CGAACGA1209.997166E-911.87702816
TCCAACG2800.011.87676818
TAGAAAT6300.011.60876754
CTCTTAA3950.011.5412151
TAGGACC12450.011.3671844
AAATGTC6450.011.3412827
AGGCCCG3100.011.33915510
ACGGTAT3050.011.2135549
TTAACGA3100.011.03365912
GTTATTC2250.010.9745993
TTAGGAC10650.010.9686543
ACGAACG1303.257992E-810.96389215
CGAGGAT3300.010.94126316
GCTCGTA1658.0035534E-1110.9398249
ACGAGGA3750.010.89567215
CGCATCG2450.010.85852113
Produced by FastQC (version 0.11.5)