##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064083_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2282781
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276578874627045	32.0	32.0	32.0	32.0	32.0
2	30.91131387548784	32.0	32.0	32.0	32.0	32.0
3	30.93349252512615	32.0	32.0	32.0	32.0	32.0
4	30.960658950639594	32.0	32.0	32.0	32.0	32.0
5	30.892212174536233	32.0	32.0	32.0	32.0	32.0
6	34.54721850234429	36.0	36.0	36.0	32.0	36.0
7	34.48061377766855	36.0	36.0	36.0	32.0	36.0
8	34.445166224880964	36.0	36.0	36.0	32.0	36.0
9	34.55702101953713	36.0	36.0	36.0	32.0	36.0
10	34.28694298752268	36.0	36.0	36.0	32.0	36.0
11	34.546531182798525	36.0	36.0	36.0	32.0	36.0
12	34.380168312247214	36.0	36.0	36.0	32.0	36.0
13	34.4466871767375	36.0	36.0	36.0	32.0	36.0
14	34.36525229533626	36.0	36.0	36.0	32.0	36.0
15	34.3148860972647	36.0	36.0	36.0	32.0	36.0
16	34.30873701857515	36.0	36.0	36.0	32.0	36.0
17	34.246563730817805	36.0	36.0	36.0	32.0	36.0
18	34.24276529373602	36.0	36.0	36.0	32.0	36.0
19	34.23525427975789	36.0	36.0	36.0	32.0	36.0
20	34.215909454301574	36.0	36.0	36.0	32.0	36.0
21	34.19685374987789	36.0	36.0	36.0	32.0	36.0
22	34.171261281743625	36.0	36.0	36.0	32.0	36.0
23	34.128993539021046	36.0	36.0	36.0	32.0	36.0
24	34.113343767974236	36.0	36.0	36.0	32.0	36.0
25	33.73330775050257	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	16.0
5	18.0
6	99.0
7	32.0
8	129.0
9	146.0
10	195.0
11	66.0
12	137.0
13	141.0
14	220.0
15	363.0
16	662.0
17	834.0
18	1098.0
19	1359.0
20	2099.0
21	3091.0
22	4805.0
23	7222.0
24	10852.0
25	16226.0
26	23174.0
27	31200.0
28	42655.0
29	58163.0
30	77533.0
31	106188.0
32	149905.0
33	213752.0
34	468899.0
35	1061492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03503410944911	17.514819763541126	11.669344967985278	25.780801159024485
2	15.571732666739388	19.978104386613975	39.70303800540512	24.747124941241513
3	18.827089645214592	24.65852320815584	28.852795685170225	27.661591461459338
4	12.198611635590703	15.917519974275917	36.721958254442356	35.16191013569102
5	13.812970631375485	37.37963139826476	34.26060601812341	14.546791952236337
6	33.02324745913009	36.34147153586046	17.564576518760685	13.070704486248767
7	29.262019507779318	30.752422260478497	21.380126531973225	18.605431699768957
8	27.03808307241283	34.78357657916244	19.582863165182943	18.595477183241783
9	27.61240342693054	14.07582618512928	18.814940842146967	39.49682954579321
10	15.622722126498875	27.42232054137287	32.60602097166769	24.348936360460566
11	36.40796529297856	20.947289894621868	23.91380575393886	18.730939058460713
12	25.014805122506633	23.50869348903369	30.238131245054912	21.23837014340476
13	29.942002036569708	20.353335639049362	25.382295169349582	24.322367155031348
14	22.907070328047933	20.499839561784494	26.01084842884521	30.582241681322365
15	24.155541197912513	29.14400881563179	22.64326935805264	24.05718062840306
16	24.124311666405113	26.079569215608743	25.961080713333224	23.83503840465292
17	22.84051951057752	26.62612255024655	26.48953382302467	24.043824116151267
18	23.3187695965994	25.525820163717093	28.425985353843565	22.729424885839943
19	25.434042961737408	24.700828959256974	26.002683848205194	23.862444230800424
20	25.511456700231832	25.159495926233795	26.013443603853858	23.315603769680518
21	25.892124004568085	24.47492267798312	25.020330594091746	24.61262272335706
22	25.390269479716682	25.645115186927768	25.476854766443314	23.487760566912232
23	23.757204018686057	25.507344865609017	26.327041074689422	24.4084100410155
24	24.585031633652015	25.296618919164192	26.513476580224882	23.60487286695891
25	24.278165455845546	25.530226540678726	26.495609960547295	23.695998042928434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	267.0
1	267.0
2	680.0
3	1093.0
4	1093.0
5	1093.0
6	3010.0
7	4927.0
8	4927.0
9	4927.0
10	5023.5
11	5120.0
12	5120.0
13	5120.0
14	5709.0
15	6298.0
16	6298.0
17	6298.0
18	11147.5
19	15997.0
20	15997.0
21	15997.0
22	26972.5
23	37948.0
24	37948.0
25	37948.0
26	60135.0
27	82322.0
28	82322.0
29	82322.0
30	108347.0
31	134372.0
32	134372.0
33	134372.0
34	152604.5
35	170837.0
36	170837.0
37	170837.0
38	188923.0
39	207009.0
40	207009.0
41	207009.0
42	228388.0
43	249767.0
44	249767.0
45	249767.0
46	272947.5
47	296128.0
48	296128.0
49	296128.0
50	296216.5
51	296305.0
52	296305.0
53	296305.0
54	271353.5
55	246402.0
56	246402.0
57	246402.0
58	222439.0
59	198476.0
60	198476.0
61	198476.0
62	172265.5
63	146055.0
64	146055.0
65	146055.0
66	118345.0
67	90635.0
68	90635.0
69	90635.0
70	68478.5
71	46322.0
72	46322.0
73	46322.0
74	34617.5
75	22913.0
76	22913.0
77	22913.0
78	17990.5
79	13068.0
80	13068.0
81	13068.0
82	9146.5
83	5225.0
84	5225.0
85	5225.0
86	3928.0
87	2631.0
88	2631.0
89	2631.0
90	1872.5
91	1114.0
92	1114.0
93	1114.0
94	789.0
95	464.0
96	464.0
97	464.0
98	775.0
99	1086.0
100	1086.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037760959110838925
2	0.005782420652703873
3	0.0021465046362309833
4	0.004161590621264151
5	0.009155499366781132
6	0.012922834034451838
7	0.022428783137760477
8	0.034869748784486995
9	0.04770497038480695
10	0.05791181896117061
11	0.05620337649559901
12	0.06465797638932513
13	0.06544648829651202
14	0.06697970589381985
15	0.0604087733339291
16	0.06776821780100675
17	0.06483320125758889
18	0.07766842285790884
19	0.07569714308994162
20	0.0808224704866564
21	0.07604759282646911
22	0.0785445471992276
23	0.0848088362396568
24	0.0799025399282717
25	0.07981492749413982
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2282781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.92822390919548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1782234503637	34.59374054896976
2	15.19299218532256	14.563462626175935
3	5.421714382020621	7.795594226195693
4	2.5093578790867146	4.810762651886877
5	1.2957608312654432	3.105175762682771
6	0.7917726835708158	2.2768955078019983
7	0.5017486198534518	1.6833544138926226
8	0.33684340237121907	1.2915444808946412
9	0.27280373870677516	1.176749880480358
>10	1.3083815166966681	11.087584770853937
>50	0.1009783049693878	3.3695007131161114
>100	0.07603704651483435	7.635842488369845
>500	0.008985095940267212	2.8596233133717113
>1k	0.004217494012778441	3.208089919724835
>5k	1.8336930490341047E-4	0.5420786955829051
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7184	0.3147038634017017	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5152	0.2256896303237148	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3634	0.15919179281762025	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3349	0.1467070209538278	No Hit
GTCCTACAGTGGACATTTCTAAATT	2954	0.12940356521278215	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2813	0.12322688860648481	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2644	0.11582363792234122	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2631	0.11525415710048402	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2483	0.10877083697472512	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2405	0.10535395204358193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2309	0.10114855520525184	No Hit
GTACATGGGGTGGTATCAACGCAAA	2294	0.10049146194926276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.380621706593843E-5
2	0.0	0.0	0.0	0.0	4.380621706593843E-5
3	0.0	0.0	0.0	0.0	4.380621706593843E-5
4	0.0	0.0	0.0	4.380621706593843E-5	4.380621706593843E-5
5	0.0	0.0	0.0	4.380621706593843E-5	4.380621706593843E-5
6	0.0	0.0	0.0	4.380621706593843E-5	4.380621706593843E-5
7	0.0	0.0	0.0	4.380621706593843E-5	4.380621706593843E-5
8	0.0	0.0	0.0	4.380621706593843E-5	4.380621706593843E-5
9	0.0	0.0	0.0	1.314186511978153E-4	4.380621706593843E-5
10	0.0	0.0	0.0	1.314186511978153E-4	4.380621706593843E-5
11	0.0	0.0	0.0	1.314186511978153E-4	8.761243413187686E-5
12	0.0	0.0	0.0	1.314186511978153E-4	8.761243413187686E-5
13	0.0	0.0	0.0	1.314186511978153E-4	1.7522486826375373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCCG	295	0.0	12.239916	15
CCAACGA	210	0.0	12.2166395	19
GCGTTAT	165	0.0	12.087674	1
CGTTATT	165	0.0	12.085555	2
CGAACGA	120	9.997166E-9	11.877028	16
TCCAACG	280	0.0	11.876768	18
TAGAAAT	630	0.0	11.6087675	4
CTCTTAA	395	0.0	11.541215	1
TAGGACC	1245	0.0	11.367184	4
AAATGTC	645	0.0	11.341282	7
AGGCCCG	310	0.0	11.339155	10
ACGGTAT	305	0.0	11.213554	9
TTAACGA	310	0.0	11.033659	12
GTTATTC	225	0.0	10.974599	3
TTAGGAC	1065	0.0	10.968654	3
ACGAACG	130	3.257992E-8	10.963892	15
CGAGGAT	330	0.0	10.941263	16
GCTCGTA	165	8.0035534E-11	10.939824	9
ACGAGGA	375	0.0	10.895672	15
CGCATCG	245	0.0	10.858521	13
>>END_MODULE