##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2282781
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316219558512184	32.0	32.0	32.0	32.0	32.0
2	31.432562738168926	32.0	32.0	32.0	32.0	32.0
3	31.50092672052203	32.0	32.0	32.0	32.0	32.0
4	31.58035615330599	32.0	32.0	32.0	32.0	32.0
5	31.536855265573	32.0	32.0	32.0	32.0	32.0
6	35.12611284218679	36.0	36.0	36.0	36.0	36.0
7	35.14521191476537	36.0	36.0	36.0	36.0	36.0
8	35.101395184207334	36.0	36.0	36.0	36.0	36.0
9	35.19973444671215	36.0	36.0	36.0	36.0	36.0
10	35.070230565262285	36.0	36.0	36.0	36.0	36.0
11	35.207034752786186	36.0	36.0	36.0	36.0	36.0
12	35.12129941505558	36.0	36.0	36.0	36.0	36.0
13	35.15641754509083	36.0	36.0	36.0	36.0	36.0
14	35.0997279195858	36.0	36.0	36.0	36.0	36.0
15	35.079213906195996	36.0	36.0	36.0	36.0	36.0
16	35.08910885450685	36.0	36.0	36.0	36.0	36.0
17	35.05495489930922	36.0	36.0	36.0	36.0	36.0
18	35.05948665246469	36.0	36.0	36.0	36.0	36.0
19	35.043869298018514	36.0	36.0	36.0	36.0	36.0
20	35.04081907112421	36.0	36.0	36.0	36.0	36.0
21	35.03738992045229	36.0	36.0	36.0	36.0	36.0
22	35.01797062442696	36.0	36.0	36.0	36.0	36.0
23	34.97120135483868	36.0	36.0	36.0	36.0	36.0
24	34.94473845717132	36.0	36.0	36.0	36.0	36.0
25	34.924411934390555	36.0	36.0	36.0	36.0	36.0
26	34.870852701157055	36.0	36.0	36.0	32.0	36.0
27	34.850516102946365	36.0	36.0	36.0	32.0	36.0
28	34.82266191982499	36.0	36.0	36.0	32.0	36.0
29	34.796982715380935	36.0	36.0	36.0	32.0	36.0
30	34.780302622108735	36.0	36.0	36.0	32.0	36.0
31	34.7735696065457	36.0	36.0	36.0	32.0	36.0
32	34.722506013498446	36.0	36.0	36.0	32.0	36.0
33	34.70595252019357	36.0	36.0	36.0	32.0	36.0
34	34.695297534016625	36.0	36.0	36.0	32.0	36.0
35	34.65462389953307	36.0	36.0	36.0	32.0	36.0
36	34.61849209363491	36.0	36.0	36.0	32.0	36.0
37	34.593788015582746	36.0	36.0	36.0	32.0	36.0
38	34.55175945480534	36.0	36.0	36.0	32.0	36.0
39	34.52270980002024	36.0	36.0	36.0	32.0	36.0
40	34.510143110530535	36.0	36.0	36.0	32.0	36.0
41	34.49738761624527	36.0	36.0	36.0	32.0	36.0
42	34.43773581434224	36.0	36.0	36.0	32.0	36.0
43	34.42376075497387	36.0	36.0	36.0	32.0	36.0
44	34.34819371634861	36.0	36.0	36.0	32.0	36.0
45	34.31104823458755	36.0	36.0	36.0	32.0	36.0
46	34.25162860563497	36.0	36.0	36.0	32.0	36.0
47	34.227264901889406	36.0	36.0	36.0	32.0	36.0
48	34.16843797105373	36.0	36.0	36.0	32.0	36.0
49	34.14108931167729	36.0	36.0	36.0	32.0	36.0
50	33.559272659094326	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	1.0
19	4.0
20	21.0
21	63.0
22	217.0
23	637.0
24	1694.0
25	3992.0
26	8192.0
27	15322.0
28	25249.0
29	39379.0
30	56710.0
31	80581.0
32	116561.0
33	186373.0
34	387959.0
35	1359823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.330301652266186	18.390124780838374	12.16321834850488	26.116355218390563
2	14.988619099936393	20.703907354742395	39.10184635171625	25.20562719360497
3	18.247245016147033	25.140180447280763	29.221797008198802	27.3907775283734
4	11.96321504340539	16.58976485260741	36.73580601906184	34.71121408492536
5	13.458233619431736	37.8985982448601	33.971852753286456	14.671315382421705
6	33.15033952127522	36.627689218332264	17.232550060825144	12.989421199567369
7	29.16857041351791	30.961954705207994	21.349241428977574	18.520233452296523
8	27.037384242020696	34.94515459220774	19.45487519603291	18.562585969738652
9	27.556989301952513	13.922750293176703	18.713194112547438	39.80706629232335
10	15.551168081108798	27.701147864362497	32.49433471134297	24.25334934318573
11	36.51955072324096	20.881118636049028	23.869448654710485	18.729881985999526
12	24.983780719711124	23.63546680205732	30.1627360009322	21.218016477299358
13	30.00792455855302	20.297076762206736	25.482427762326328	24.21257091691392
14	22.89073605589688	20.54657160692578	25.97991479668386	30.582777540493478
15	24.20877168772739	29.21003116943424	22.609733712711126	23.97146343012724
16	24.1375231792083	26.08867260071922	25.86543377153153	23.908370448540953
17	22.97452098996794	26.734934275342226	26.354959148512275	23.93558558617756
18	23.428603219410736	25.51912625756863	28.385677450895358	22.66659307212528
19	25.53285905781547	24.721874206011968	26.03290724467535	23.71235949149721
20	25.65809446000793	25.15515175675113	25.988702297433157	23.19805148580778
21	26.009634739381482	24.483995617626046	25.100699541480324	24.405670101512147
22	25.423607046858738	25.609608651922045	25.574344569944664	23.392439731274553
23	23.757942670401903	25.448348822590148	26.334291966102512	24.459416540905437
24	24.7328464978951	25.275608142738864	26.45864454150002	23.532900817866015
25	24.449007181272705	25.414818926177844	26.422729146469838	23.713444746079606
26	23.344745482782113	26.27961737538086	27.04083621922717	23.334800922609855
27	24.060889199251044	25.900515105767973	26.18956663299218	23.849029061988805
28	23.45949431635981	25.992249501903892	26.285543559972176	24.262712621764127
29	23.958666907899033	25.31270767412428	25.87944310369677	24.84918231427992
30	23.547857445757124	25.692733504592812	26.512246922060577	24.24716212758948
31	24.7112523525639	25.146498578823596	25.856736306062867	24.285512762549637
32	23.864541885028913	26.137845624920875	25.783793232000697	24.213819258049522
33	23.52044396975833	25.787693226990406	26.572648441873977	24.119214361377296
34	24.244684966156647	25.80155367076952	26.596831941501442	23.356929421572396
35	24.234949122189104	25.973398448520275	26.51306071167605	23.278591717614567
36	23.439229097064693	26.061257623907945	26.45321914885601	24.046294130171354
37	24.045973748562005	25.946359871274904	25.601153724502712	24.40651265566037
38	24.09898562775509	26.35887065330059	25.66154997340745	23.880593745536878
39	24.803410730729432	25.214682053272515	25.76609578749171	24.215811428506342
40	25.342264720931375	25.511184823825445	26.05632013513608	23.0902303201071
41	24.262105775936693	25.392997452153406	26.769561819449848	23.575334952460057
42	24.628165499646038	26.50950438421263	26.02153731311899	22.84079280302234
43	23.629711369931606	26.030500944256246	26.44031384966109	23.89947383615106
44	23.311329239161243	26.376351052696368	25.876297827583052	24.43602188055934
45	23.5940526093758	26.428091743770338	25.886245049280493	24.091610597573364
46	23.710080652403516	25.454504833843604	25.912196600692404	24.923217913060466
47	24.6279909231888	25.612859984054246	25.90255570060541	23.856593392151538
48	24.232108374833096	26.088645945406054	25.305159054190923	24.374086625569927
49	24.210685863202343	25.964561522323947	25.456276204423308	24.36847641005041
50	23.88718114510682	26.917488157866494	25.08582890991918	24.109501787107508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	48.0
2	51.0
3	159.0
4	267.0
5	301.5
6	336.0
7	349.5
8	363.0
9	542.5
10	722.0
11	1096.0
12	1470.0
13	2697.0
14	3924.0
15	5440.0
16	6956.0
17	7671.0
18	8386.0
19	8480.5
20	8575.0
21	9356.5
22	10138.0
23	11459.0
24	12780.0
25	14422.0
26	16064.0
27	21547.5
28	27031.0
29	33284.0
30	39537.0
31	46925.5
32	54314.0
33	65600.5
34	76887.0
35	90070.5
36	103254.0
37	109730.5
38	116207.0
39	116502.0
40	116797.0
41	119022.0
42	121247.0
43	121523.0
44	121799.0
45	129877.5
46	137956.0
47	145750.5
48	153545.0
49	158004.5
50	162464.0
51	159317.5
52	156171.0
53	154077.0
54	151983.0
55	150184.0
56	148385.0
57	143682.5
58	138980.0
59	125993.0
60	113006.0
61	99125.5
62	85245.0
63	72892.5
64	60540.0
65	50946.5
66	41353.0
67	34972.0
68	28591.0
69	25230.5
70	21870.0
71	17130.5
72	12391.0
73	10508.0
74	8625.0
75	6869.5
76	5114.0
77	4422.5
78	3731.0
79	3052.0
80	2373.0
81	1935.0
82	1497.0
83	1199.0
84	901.0
85	675.0
86	449.0
87	310.0
88	171.0
89	134.0
90	97.0
91	82.5
92	68.0
93	49.0
94	30.0
95	37.5
96	45.0
97	32.5
98	20.0
99	25.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009593561537440516
2	3.942559535934459E-4
3	3.942559535934459E-4
4	0.0
5	0.0
6	3.5044973652750746E-4
7	8.761243413187686E-5
8	4.380621706593843E-5
9	0.001226574077846276
10	8.761243413187688E-4
11	4.380621706593843E-5
12	1.7522486826375373E-4
13	1.7522486826375373E-4
14	2.628373023956306E-4
15	0.0042053968383300895
16	0.0010513492095825224
17	0.0
18	1.314186511978153E-4
19	4.380621706593843E-5
20	7.008994730550149E-4
21	0.0
22	2.190310853296922E-4
23	7.008994730550149E-4
24	4.818683877253227E-4
25	0.0016646362485056604
26	0.005519583350308242
27	0.006264289040429195
28	0.0038987533188685206
29	0.002540760589824429
30	0.0037235284506047664
31	0.007228025815879841
32	0.0029788227604838134
33	0.004512040357791658
34	0.002540760589824429
35	0.0024969543727584908
36	0.006965188513484211
37	0.003811140884736644
38	0.008191762591330486
39	0.004512040357791658
40	0.0019712797679672296
41	0.0030226289775497515
42	0.0019712797679672296
43	0.0014017989461100298
44	0.0010513492095825224
45	0.0014017989461100298
46	9.637367754506454E-4
47	0.0017960548997034756
48	0.0016208300314397219
49	3.5044973652750746E-4
50	0.001839861116769414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2282781.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.63644304502768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5920475338482	38.73623618582371
2	14.723931201467119	15.500307321698603
3	5.092354645477072	8.041303057852083
4	2.3490987277031294	4.945928055515711
5	1.2710285081250585	3.345120983826557
6	0.7097088647953654	2.24139301442115
7	0.4451533845598511	1.6401903540880138
8	0.32474319002747476	1.367466114091342
9	0.23401087922878572	1.1085750284798548
>10	1.116027014132882	10.336108824856126
>50	0.07497973920866147	2.7455092840150597
>100	0.058115984347874856	6.203445086163344
>500	0.006537385829692096	2.2980902505086074
>1k	0.002262941248739338	1.4903264386599784
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.380621706593843E-5	0.0
8	0.0	0.0	0.0	4.380621706593843E-5	0.0
9	0.0	0.0	0.0	8.761243413187686E-5	0.0
10	0.0	0.0	0.0	8.761243413187686E-5	0.0
11	0.0	0.0	0.0	8.761243413187686E-5	0.0
12	0.0	0.0	0.0	8.761243413187686E-5	4.380621706593843E-5
13	0.0	0.0	0.0	8.761243413187686E-5	1.314186511978153E-4
14	0.0	0.0	0.0	8.761243413187686E-5	1.314186511978153E-4
15	0.0	0.0	0.0	8.761243413187686E-5	3.06643519461569E-4
16	0.0	0.0	0.0	8.761243413187686E-5	4.380621706593843E-4
17	0.0	0.0	0.0	2.1903108532969216E-4	4.8186838772532276E-4
18	0.0	0.0	0.0	2.628373023956306E-4	4.8186838772532276E-4
19	0.0	0.0	0.0	2.628373023956306E-4	4.8186838772532276E-4
20	0.0	0.0	0.0	3.5044973652750746E-4	5.694808218571996E-4
21	0.0	0.0	0.0	4.380621706593843E-4	5.694808218571996E-4
22	0.0	0.0	0.0	6.13287038923138E-4	5.694808218571996E-4
23	0.0	0.0	0.0	0.001226574077846276	5.694808218571996E-4
24	0.0	0.0	0.0	0.0019712797679672296	6.570932559890765E-4
25	0.0	0.0	0.0	0.002321729504494737	6.570932559890765E-4
26	0.0	0.0	0.0	0.0025845668068903674	7.447056901209534E-4
27	0.0	0.0	0.0	0.0031102414116816286	7.447056901209534E-4
28	0.0	0.0	0.0	0.005169133613780735	7.447056901209534E-4
29	0.0	0.0	0.0	0.009330724235044887	7.447056901209534E-4
30	0.0	0.0	0.0	0.016295912748529096	7.447056901209534E-4
31	0.0	0.0	0.0	0.03811140884736643	7.447056901209534E-4
32	0.0	0.0	0.0	0.06356282096267667	7.447056901209534E-4
33	0.0	0.0	0.0	0.08691153465882184	7.447056901209534E-4
34	0.0	0.0	0.0	0.11122398513041767	7.447056901209534E-4
35	0.0	0.0	0.0	0.14057415056459643	7.447056901209534E-4
36	0.0	0.0	0.0	0.1846432049329305	7.885119071868918E-4
37	0.0	0.0	0.0	0.2520171667803438	7.885119071868918E-4
38	0.0	0.0	0.0	0.339410569826891	8.323181242528302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	85	6.1177216E-6	20.705738	5
TTAGGAC	1550	0.0	20.295742	3
GATATAC	1150	0.0	19.513334	1
GTATCAA	5145	0.0	18.857426	1
TAGGACG	2270	0.0	18.704716	4
TGTAGGA	2810	0.0	18.475931	2
GACGTGA	1360	0.0	18.441048	7
GTAGGAC	2685	0.0	18.270954	3
CTGTAGG	2740	0.0	18.22655	1
ACTGTTC	1260	0.0	18.158602	8
AGGACGT	2320	0.0	18.017115	5
CTTTAGG	1790	0.0	17.821495	1
AAATTTA	1275	0.0	17.772036	44
GGACGTG	2365	0.0	17.767317	6
ATACACT	1320	0.0	17.666544	4
GTCCTAC	2295	0.0	17.159298	1
TAGGACC	1710	0.0	17.110992	4
AATCCCG	790	0.0	16.986479	19
GTCCTAT	725	0.0	16.689905	1
TAGGACA	910	0.0	16.681204	4
>>END_MODULE