FastQCFastQC Report
Thu 2 Feb 2017
SRR4064082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199201
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30780.25667090004094395No Hit
TATCAACGCAGAGTACTTTTTTTTT21210.17686776445316507No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18980.15827204947294074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA755.8285877E-815.20273710
GCGTAAC755.83168E-815.20210211
ACGGTAT1058.913048E-1114.4794029
CGTCTTA602.564954E-514.25137615
TCGCGTA852.6899943E-713.414749
GTAGTAA500.001502352413.296291
GCGTTAT604.1004477E-412.6631351
GCCGTTA550.003065521612.09207715
CCGTTAA550.003065521612.09207716
TAGAGTT550.00307415612.0875385
GAATAAG550.00307415612.0875381
TTAACGA1501.4551915E-1112.03499812
GTCTTAG1201.00426405E-811.8716891
CGACCCG1459.822543E-1111.7898165
ATGCCCG658.039859E-411.6890475
GCGGTCG907.4380714E-611.6136879
CGCCCCT1157.093877E-811.5619935
GTATTAT752.0784377E-411.396821
TAATATA2500.011.3963464
GTATCAA19450.011.1819711
Produced by FastQC (version 0.11.5)