##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064082_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199201
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266189738000552	32.0	32.0	32.0	32.0	32.0
2	30.884703231568352	32.0	32.0	32.0	32.0	32.0
3	30.899056121534255	32.0	32.0	32.0	32.0	32.0
4	30.923420677601168	32.0	32.0	32.0	32.0	32.0
5	30.845896559459174	32.0	32.0	32.0	32.0	32.0
6	34.503429366720006	36.0	36.0	36.0	32.0	36.0
7	34.440417411259666	36.0	36.0	36.0	32.0	36.0
8	34.39778819397249	36.0	36.0	36.0	32.0	36.0
9	34.53265799478152	36.0	36.0	36.0	32.0	36.0
10	34.239789659948585	36.0	36.0	36.0	32.0	36.0
11	34.51489033114549	36.0	36.0	36.0	32.0	36.0
12	34.33451773305726	36.0	36.0	36.0	32.0	36.0
13	34.41189175125771	36.0	36.0	36.0	32.0	36.0
14	34.31282412206127	36.0	36.0	36.0	32.0	36.0
15	34.26158417146083	36.0	36.0	36.0	32.0	36.0
16	34.25912586797376	36.0	36.0	36.0	32.0	36.0
17	34.175336745049414	36.0	36.0	36.0	32.0	36.0
18	34.1691893185546	36.0	36.0	36.0	32.0	36.0
19	34.180658621865724	36.0	36.0	36.0	32.0	36.0
20	34.16439529319939	36.0	36.0	36.0	32.0	36.0
21	34.145111620153756	36.0	36.0	36.0	32.0	36.0
22	34.12757410976142	36.0	36.0	36.0	32.0	36.0
23	34.08207131248223	36.0	36.0	36.0	32.0	36.0
24	34.05635752471854	36.0	36.0	36.0	32.0	36.0
25	33.672449405896096	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	7.0
5	17.0
6	48.0
7	26.0
8	70.0
9	77.0
10	111.0
11	24.0
12	85.0
13	63.0
14	106.0
15	207.0
16	324.0
17	421.0
18	607.0
19	777.0
20	1253.0
21	1795.0
22	2659.0
23	4248.0
24	6140.0
25	9238.0
26	12801.0
27	17242.0
28	23104.0
29	31302.0
30	41122.0
31	56489.0
32	79866.0
33	113345.0
34	246382.0
35	549241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.18685602594124	16.666096608935113	10.884126172407148	26.262921192716497
2	17.165137507567962	19.767515557060346	36.62117828366138	26.446168651710316
3	18.973449379201305	23.63671539158809	28.37061294333382	29.01922228587678
4	12.938697257300397	15.106238017524124	35.31385226331717	36.6412124618583
5	14.951592175020412	36.21467404675073	33.31929482779758	15.51443895043129
6	34.3847317599609	34.90606636320116	16.85501210136793	13.854189775470003
7	30.651554151326444	30.16861870453192	20.721934277406795	18.45789286673484
8	28.638915142935556	32.16198111877553	19.46532950381103	19.73377423447788
9	27.945712471664407	13.829019482204375	18.195349333920692	40.029918712210524
10	16.09484943124217	26.419015944243124	31.405211061595057	26.080923562919644
11	37.33036299086787	20.759606679933086	22.513944339453406	19.396085989745643
12	25.17996597189135	23.158723019706766	28.613734238635676	23.047576769766216
13	29.88463066124171	19.188142308321346	24.98589800502665	25.941329025410294
14	24.01945281449148	19.10392886438187	25.033732842562546	31.842885478564103
15	25.47490191562825	26.62546372088133	22.150379904844392	25.749254458646025
16	25.997605130194966	25.23041234317566	23.54457420132761	25.22740832530176
17	24.44389557636433	25.251621710476908	24.955650161128013	25.348832552030746
18	25.105923804961666	24.563807565425737	25.77714315757673	24.553125472035862
19	25.862441615838268	24.543253724193928	25.160204387334527	24.434100272633277
20	26.159349110401365	23.88242058958286	24.707716420040445	25.25051387997533
21	26.942149036206715	23.84772917316901	24.275006133761057	24.935115656863218
22	26.26743878430695	23.79936624931255	24.52883882753619	25.404356138844307
23	24.87708636044597	23.812655493786924	25.175951573987295	26.134306571779813
24	25.239581151308133	24.401933841134934	24.96442690385959	25.394058103697347
25	25.2040682628264	23.94381477670371	25.060358806293504	25.79175815417638
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	320.0
1	320.0
2	477.0
3	634.0
4	634.0
5	634.0
6	1585.0
7	2536.0
8	2536.0
9	2536.0
10	2523.5
11	2511.0
12	2511.0
13	2511.0
14	2642.0
15	2773.0
16	2773.0
17	2773.0
18	4680.0
19	6587.0
20	6587.0
21	6587.0
22	10559.0
23	14531.0
24	14531.0
25	14531.0
26	21833.5
27	29136.0
28	29136.0
29	29136.0
30	36982.0
31	44828.0
32	44828.0
33	44828.0
34	56466.5
35	68105.0
36	68105.0
37	68105.0
38	80043.0
39	91981.0
40	91981.0
41	91981.0
42	107161.5
43	122342.0
44	122342.0
45	122342.0
46	138650.5
47	154959.0
48	154959.0
49	154959.0
50	161254.0
51	167549.0
52	167549.0
53	167549.0
54	157725.0
55	147901.0
56	147901.0
57	147901.0
58	136515.5
59	125130.0
60	125130.0
61	125130.0
62	109749.0
63	94368.0
64	94368.0
65	94368.0
66	76932.5
67	59497.0
68	59497.0
69	59497.0
70	45304.5
71	31112.0
72	31112.0
73	31112.0
74	23521.0
75	15930.0
76	15930.0
77	15930.0
78	12586.5
79	9243.0
80	9243.0
81	9243.0
82	6414.5
83	3586.0
84	3586.0
85	3586.0
86	2728.0
87	1870.0
88	1870.0
89	1870.0
90	1344.5
91	819.0
92	819.0
93	819.0
94	561.5
95	304.0
96	304.0
97	304.0
98	476.5
99	649.0
100	649.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04102731735547252
2	0.005587053379708656
3	0.0016677771282712407
4	0.00450299824633235
5	0.00983988505680032
6	0.01359238359541061
7	0.023599046365038054
8	0.03702465224762154
9	0.051701090976408466
10	0.059372865766456166
11	0.05720475549970355
12	0.06504330800257838
13	0.06579380771030044
14	0.06787852912063949
15	0.06195792031527659
16	0.067211418269331
17	0.06462636372051057
18	0.07788519189026694
19	0.07404930449524308
20	0.0803868575826738
21	0.07630080361840925
22	0.07821874731592118
23	0.08355563412638915
24	0.0803868575826738
25	0.07921941359288394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1199201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.630907782198825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.12309302349777	43.460585836503455
2	12.740830100321421	14.17567888759288
3	3.854830992734395	6.4334324241830725
4	1.6856424573844477	3.750952804020529
5	0.9249115829487154	2.5726835488853763
6	0.5642331991311844	1.8833283041113165
7	0.38968992311589395	1.5175162923558712
8	0.2792366160876274	1.2427349151187246
9	0.21884939856153135	1.0957311638609601
>10	1.0757117733634023	10.439008754650226
>50	0.0681252605288256	2.652662107413679
>100	0.06821950139164029	7.639651126920528
>500	0.0054214125816465526	2.0887344204002876
>1k	0.0012047583514770118	1.0472994139829934
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3078	0.25667090004094395	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2121	0.17686776445316507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1898	0.15827204947294074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6677771282712405E-4
2	0.0	0.0	0.0	0.0	1.6677771282712405E-4
3	0.0	0.0	0.0	0.0	1.6677771282712405E-4
4	0.0	0.0	0.0	0.0	1.6677771282712405E-4
5	0.0	0.0	0.0	0.0	1.6677771282712405E-4
6	0.0	0.0	0.0	0.0	1.6677771282712405E-4
7	0.0	0.0	0.0	0.0	1.6677771282712405E-4
8	0.0	0.0	0.0	0.0	1.6677771282712405E-4
9	0.0	0.0	0.0	0.0	1.6677771282712405E-4
10	0.0	0.0	0.0	8.338885641356202E-5	1.6677771282712405E-4
11	0.0	0.0	0.0	8.338885641356202E-5	1.6677771282712405E-4
12	0.0	0.0	0.0	8.338885641356202E-5	2.5016656924068607E-4
13	0.0	0.0	0.0	8.338885641356202E-5	3.335554256542481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	75	5.8285877E-8	15.202737	10
GCGTAAC	75	5.83168E-8	15.202102	11
ACGGTAT	105	8.913048E-11	14.479402	9
CGTCTTA	60	2.564954E-5	14.251376	15
TCGCGTA	85	2.6899943E-7	13.41474	9
GTAGTAA	50	0.0015023524	13.29629	1
GCGTTAT	60	4.1004477E-4	12.663135	1
GCCGTTA	55	0.0030655216	12.092077	15
CCGTTAA	55	0.0030655216	12.092077	16
TAGAGTT	55	0.003074156	12.087538	5
GAATAAG	55	0.003074156	12.087538	1
TTAACGA	150	1.4551915E-11	12.034998	12
GTCTTAG	120	1.00426405E-8	11.871689	1
CGACCCG	145	9.822543E-11	11.789816	5
ATGCCCG	65	8.039859E-4	11.689047	5
GCGGTCG	90	7.4380714E-6	11.613687	9
CGCCCCT	115	7.093877E-8	11.561993	5
GTATTAT	75	2.0784377E-4	11.39682	1
TAATATA	250	0.0	11.396346	4
GTATCAA	1945	0.0	11.181971	1
>>END_MODULE