##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199201
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319973048721607	32.0	32.0	32.0	32.0	32.0
2	31.448870539634306	32.0	32.0	32.0	32.0	32.0
3	31.5005441122881	32.0	32.0	32.0	32.0	32.0
4	31.578094080975582	32.0	32.0	32.0	32.0	32.0
5	31.53210179110925	32.0	32.0	32.0	32.0	32.0
6	35.12008662434404	36.0	36.0	36.0	36.0	36.0
7	35.137208024342875	36.0	36.0	36.0	36.0	36.0
8	35.090410198123585	36.0	36.0	36.0	36.0	36.0
9	35.182656618865394	36.0	36.0	36.0	36.0	36.0
10	35.05142757552737	36.0	36.0	36.0	36.0	36.0
11	35.18268080163376	36.0	36.0	36.0	36.0	36.0
12	35.09531429676927	36.0	36.0	36.0	36.0	36.0
13	35.12793351573256	36.0	36.0	36.0	36.0	36.0
14	35.07908682531119	36.0	36.0	36.0	36.0	36.0
15	35.055676237761645	36.0	36.0	36.0	36.0	36.0
16	35.06718056439246	36.0	36.0	36.0	36.0	36.0
17	35.02530351459013	36.0	36.0	36.0	36.0	36.0
18	35.02273430392403	36.0	36.0	36.0	36.0	36.0
19	35.019522165174976	36.0	36.0	36.0	36.0	36.0
20	35.01297947550077	36.0	36.0	36.0	36.0	36.0
21	35.01238074351172	36.0	36.0	36.0	36.0	36.0
22	34.99892011430944	36.0	36.0	36.0	36.0	36.0
23	34.948122124647995	36.0	36.0	36.0	36.0	36.0
24	34.911593636096036	36.0	36.0	36.0	32.0	36.0
25	34.88779612425273	36.0	36.0	36.0	32.0	36.0
26	34.834082860171065	36.0	36.0	36.0	32.0	36.0
27	34.82267192905943	36.0	36.0	36.0	32.0	36.0
28	34.80043462271963	36.0	36.0	36.0	32.0	36.0
29	34.773635945934004	36.0	36.0	36.0	32.0	36.0
30	34.757956339262556	36.0	36.0	36.0	32.0	36.0
31	34.74610678276619	36.0	36.0	36.0	32.0	36.0
32	34.711450374040716	36.0	36.0	36.0	32.0	36.0
33	34.681668043972614	36.0	36.0	36.0	32.0	36.0
34	34.66881782119928	36.0	36.0	36.0	32.0	36.0
35	34.63018209624575	36.0	36.0	36.0	32.0	36.0
36	34.60512958211342	36.0	36.0	36.0	32.0	36.0
37	34.590985164288554	36.0	36.0	36.0	32.0	36.0
38	34.554671818986144	36.0	36.0	36.0	32.0	36.0
39	34.551025224295174	36.0	36.0	36.0	32.0	36.0
40	34.52522804767508	36.0	36.0	36.0	32.0	36.0
41	34.51214767165805	36.0	36.0	36.0	32.0	36.0
42	34.43485787620257	36.0	36.0	36.0	32.0	36.0
43	34.42483787121592	36.0	36.0	36.0	32.0	36.0
44	34.362734020401916	36.0	36.0	36.0	32.0	36.0
45	34.32864048645723	36.0	36.0	36.0	32.0	36.0
46	34.30350625124562	36.0	36.0	36.0	32.0	36.0
47	34.266093006927115	36.0	36.0	36.0	32.0	36.0
48	34.22528917170683	36.0	36.0	36.0	32.0	36.0
49	34.201962806902266	36.0	36.0	36.0	32.0	36.0
50	33.60071580994345	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	13.0
21	38.0
22	130.0
23	384.0
24	1094.0
25	2395.0
26	4788.0
27	8539.0
28	13957.0
29	21332.0
30	30188.0
31	42087.0
32	59827.0
33	95296.0
34	199046.0
35	720083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15371374530267	17.56612953532941	11.516438353879538	26.76371836548838
2	16.35747313823023	20.417446703830485	36.206872110040486	27.0182080478988
3	18.16529199907605	24.26252857115458	28.704410193804364	28.867769235965007
4	12.571287048626544	15.6957841095863	35.45210519337459	36.28082364841257
5	14.452623038172918	36.629555845934085	33.19952201507504	15.718299100817962
6	34.44927359785457	35.276576511968834	16.594173772804822	13.679976117371776
7	30.44085186720158	30.329110799607406	20.760573081576815	18.469464251614202
8	28.553511879993426	32.32385563387623	19.346298076802803	19.776334409327546
9	27.901185975106362	13.63729609016813	18.138364318343672	40.32315361638184
10	15.926110001100746	26.562223771418658	31.54242704229862	25.969239185181976
11	37.42333437013478	20.74314481058638	22.499230737799586	19.334290081479253
12	25.153039653969024	23.201153436585585	28.544803656440678	23.101003253004716
13	29.859355299234817	19.134133649990996	25.002334905503037	26.004176145271153
14	24.02553704597819	18.986173270658163	25.09683547101023	31.891454212353416
15	25.571944887812574	26.60450617685468	22.062720884890513	25.760828050442232
16	26.0313611164851	25.320632559256566	23.432277733674006	25.215728590584323
17	24.454636424282857	25.36465977318212	24.921531020680455	25.259172781854573
18	25.12568848269047	24.51786406714504	25.848756874403243	24.50769057576124
19	25.93153769179453	24.361157438292196	25.333805870580388	24.373498999332888
20	26.164516185522046	23.752552766911638	24.82911367599269	25.25381737157362
21	26.95069467086835	23.769076243265307	24.385236503305116	24.894992582561223
22	26.260384023322253	23.7275245622491	24.703843735563268	25.308247678865374
23	24.902955409104973	23.689220482808825	25.236597005814755	26.171227102271448
24	25.294405094071205	24.318002531692017	25.074007795209795	25.313584579026983
25	25.37734244181004	23.86438687899024	25.162778441196288	25.59549223800343
26	25.195222592115705	24.55935233800111	25.33974234954267	24.90568272034051
27	25.620701042757737	24.265633026666045	24.721130688300164	25.392535242276054
28	25.110662272420992	24.065249878246483	25.324898427544984	25.499189421787545
29	25.08291116809473	24.335730814935268	25.207580211395335	25.373777805574665
30	24.945273689925106	24.554579956285664	25.348809864328786	25.15133648946044
31	25.509730092609967	24.30642598916701	24.595972863320032	25.58787105490299
32	25.33052358404814	24.404206951877903	24.446069658877313	25.819199805196646
33	25.039152733186004	24.05453863153067	25.01071592377934	25.895592711503983
34	25.665104200978504	24.064084164670152	25.071194898484205	25.199616735867135
35	26.017694096769645	24.05122932193199	25.105970277453242	24.825106303845118
36	25.196668498597695	24.571032083255844	24.760174097384784	25.472125320761673
37	25.775333107620156	24.40779477668572	24.594842064708523	25.222030050985605
38	25.094821432145547	24.348509210271736	25.056625991580322	25.50004336600239
39	25.655195993812256	24.24969457405068	24.550075261957478	25.545034170179587
40	25.89875562686485	24.317229255861193	25.01588614677966	24.768128970494296
41	25.008902057355105	24.575098756547288	25.282176455038147	25.133822731059464
42	26.013674378344483	24.705944160036424	24.65982919952601	24.620552262093085
43	25.313337547302922	24.079500594569982	24.990034907011925	25.61712695111517
44	25.070610456268216	24.543752631995897	24.626058531419257	25.759578380316633
45	25.134362311673865	24.69539678264285	24.67996961264461	25.49027129303868
46	24.934914400553705	24.510044279888927	24.820337060849408	25.734704258707964
47	25.21229142987232	24.407322688810744	25.13590570866519	25.244480172651745
48	25.76992387278942	24.896074557800894	24.256552619004534	25.077448950405152
49	25.051096692203203	24.89157327778505	24.437686218361122	25.619643811650626
50	25.106780766286462	25.06758751353001	24.470599223139697	25.35503249704383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	88.5
2	60.0
3	170.5
4	281.0
5	306.0
6	331.0
7	299.5
8	268.0
9	370.5
10	473.0
11	732.0
12	991.0
13	1653.5
14	2316.0
15	3004.5
16	3693.0
17	3797.0
18	3901.0
19	3836.0
20	3771.0
21	4009.0
22	4247.0
23	4620.5
24	4994.0
25	5436.0
26	5878.0
27	7090.0
28	8302.0
29	9891.5
30	11481.0
31	13380.0
32	15279.0
33	18033.5
34	20788.0
35	24372.0
36	27956.0
37	33132.5
38	38309.0
39	42199.5
40	46090.0
41	51523.5
42	56957.0
43	59753.0
44	62549.0
45	69200.5
46	75852.0
47	81694.5
48	87537.0
49	91224.0
50	94911.0
51	93839.5
52	92768.0
53	92405.0
54	92042.0
55	92301.0
56	92560.0
57	89260.5
58	85961.0
59	79051.5
60	72142.0
61	63668.5
62	55195.0
63	48181.5
64	41168.0
65	34855.5
66	28543.0
67	24406.0
68	20269.0
69	17970.0
70	15671.0
71	12325.5
72	8980.0
73	7604.5
74	6229.0
75	4950.0
76	3671.0
77	3178.5
78	2686.0
79	2241.5
80	1797.0
81	1405.0
82	1013.0
83	831.0
84	649.0
85	458.5
86	268.0
87	192.0
88	116.0
89	90.0
90	64.0
91	45.0
92	26.0
93	20.0
94	14.0
95	18.0
96	22.0
97	15.0
98	8.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009589718487559634
2	5.003331384813723E-4
3	1.6677771282712408E-4
4	0.0
5	0.0
6	2.501665692406861E-4
7	0.0
8	0.0
9	0.0015843882718576787
10	0.0010840551333763063
11	0.0
12	1.6677771282712408E-4
13	7.504997077220583E-4
14	1.6677771282712408E-4
15	0.003919276251437415
16	7.504997077220583E-4
17	8.338885641356204E-5
18	5.003331384813723E-4
19	8.338885641356204E-5
20	0.0010006662769627445
21	0.0
22	2.501665692406861E-4
23	8.338885641356203E-4
24	2.501665692406861E-4
25	0.0020847214103390504
26	0.004753164815573036
27	0.006087386518190028
28	0.004753164815573036
29	0.0021681102667526126
30	0.004336220533505225
31	0.0071714416515663345
32	0.0035857208257831672
33	0.0042528316770916634
34	0.002334887979579737
35	0.003669109682196729
36	0.008505663354183327
37	0.0032521654001289187
38	0.008755829923424014
39	0.004669775959159474
40	0.0035857208257831672
41	0.0033355542565424814
42	0.002001332553925489
43	0.0012508328462034304
44	0.0013342217026169926
45	0.0015009994154441166
46	9.172774205491823E-4
47	0.002001332553925489
48	0.002001332553925489
49	3.3355542565424815E-4
50	0.0012508328462034304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1199201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.334859437092945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54827134593249	47.199854987746335
2	12.325133293751648	14.626201030563768
3	3.489817494350811	6.212034914652398
4	1.4895854350259112	3.5353736962721363
5	0.8230962266307212	2.441914945516772
6	0.5083338208896249	1.8097149485763762
7	0.33154432283946683	1.3770495054992151
8	0.24625448269470596	1.1689180093155533
9	0.18703413776365233	0.9987879846729769
>10	0.933287111290298	9.657853655083096
>50	0.05587628143910564	2.3041906107805157
>100	0.05709110152336399	6.697815626518428
>500	0.004249950789281271	1.7058475674480398
>1k	4.249950789281271E-4	0.264442517354466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.338885641356202E-5
2	0.0	0.0	0.0	0.0	8.338885641356202E-5
3	0.0	0.0	0.0	0.0	8.338885641356202E-5
4	0.0	0.0	0.0	0.0	8.338885641356202E-5
5	0.0	0.0	0.0	0.0	8.338885641356202E-5
6	0.0	0.0	0.0	0.0	8.338885641356202E-5
7	0.0	0.0	0.0	0.0	8.338885641356202E-5
8	0.0	0.0	0.0	8.338885641356202E-5	8.338885641356202E-5
9	0.0	0.0	0.0	8.338885641356202E-5	8.338885641356202E-5
10	0.0	0.0	0.0	8.338885641356202E-5	8.338885641356202E-5
11	0.0	0.0	0.0	8.338885641356202E-5	8.338885641356202E-5
12	0.0	0.0	0.0	8.338885641356202E-5	6.671108513084962E-4
13	0.0	0.0	0.0	8.338885641356202E-5	6.671108513084962E-4
14	0.0	0.0	0.0	8.338885641356202E-5	6.671108513084962E-4
15	0.0	0.0	0.0	1.6677771282712405E-4	0.0010006662769627443
16	0.0	0.0	0.0	1.6677771282712405E-4	0.0010840551333763063
17	0.0	0.0	0.0	1.6677771282712405E-4	0.0011674439897898683
18	0.0	0.0	0.0	2.5016656924068607E-4	0.0011674439897898683
19	0.0	0.0	0.0	2.5016656924068607E-4	0.0011674439897898683
20	0.0	0.0	0.0	2.5016656924068607E-4	0.0013342217026169924
21	0.0	0.0	0.0	4.169442820678102E-4	0.0014176105590305544
22	0.0	0.0	0.0	9.172774205491824E-4	0.0014176105590305544
23	0.0	0.0	0.0	0.0021681102667526126	0.0014176105590305544
24	0.0	0.0	0.0	0.0033355542565424814	0.0014176105590305544
25	0.0	0.0	0.0	0.0035857208257831672	0.0014176105590305544
26	0.0	0.0	0.0	0.004419609389918788	0.0015843882718576787
27	0.0	0.0	0.0	0.005503664523295094	0.0015843882718576787
28	0.0	0.0	0.0	0.010090051626041006	0.0015843882718576787
29	0.0	0.0	0.0	0.01767843755967515	0.0016677771282712407
30	0.0	0.0	0.0	0.030603710303777266	0.0016677771282712407
31	0.0	0.0	0.0	0.06429280829485633	0.0016677771282712407
32	0.0	0.0	0.0	0.09956629455779306	0.0016677771282712407
33	0.0	0.0	0.0	0.13258828169756362	0.0016677771282712407
34	0.0	0.0	0.0	0.1693627673759445	0.0016677771282712407
35	0.0	0.0	0.0	0.21222463957251536	0.0016677771282712407
36	0.0	0.0	0.0	0.27068022791842233	0.0016677771282712407
37	0.0	0.0	0.0	0.3654933576606424	0.0016677771282712407
38	0.0	0.0	0.0	0.4954965848093856	0.0016677771282712407
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	80	8.991179E-5	19.249252	4
CGAACGT	185	1.8189894E-12	19.026289	4
TCTATAC	140	4.083631E-9	18.856411	3
GTATCAA	2275	0.0	18.760494	1
GTATAGG	100	2.4829254E-5	17.60005	1
ATACCGT	300	0.0	17.599316	6
ACCGTCG	305	0.0	17.310802	8
CTATTAC	115	4.2727097E-6	17.217442	1
CGTCGTA	350	0.0	16.97006	10
CCCTAAG	145	1.2491546E-7	16.689703	1
TATAGGG	95	3.3483404E-4	16.20922	2
TACCGTC	330	0.0	15.999379	7
GAACGTC	220	1.8189894E-11	15.999378	5
GTCCTAC	265	0.0	15.773629	1
AACGCAG	2740	0.0	15.656326	6
TCTAGCC	155	2.6683483E-7	15.612297	3
TAGGACG	155	2.6683483E-7	15.612297	4
ATAGCAC	100	4.945111E-4	15.399402	3
TATATAG	100	4.945111E-4	15.399402	5
AATTCCG	300	0.0	15.399401	42
>>END_MODULE