FastQCFastQC Report
Thu 2 Feb 2017
SRR4064081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387939
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35190.2535414020356802No Hit
TATCAACGCAGAGTACTTTTTTTTT25150.1812039289911156No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17070.12298811403094806No Hit
GAATAGGACCGCGGTTCTATTTTGT16170.11650367919627592No Hit
GCGCAAGACGGACCAGAGCGAAAGC15800.11383785598646627No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15730.11333351105488065No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15470.11146022988041981No Hit
GTATCTGATCGTCTTCGAACCTCCG15080.10865030811872856No Hit
GATTAAGAGGGACGGCCGGGGGCAT15040.10836211101496536No Hit
GAACTACGACGGTATCTGATCGTCT14540.10475964721792527No Hit
GTCCTATTCCATTATTCCTAGCTGC14230.10252611966376043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2000.013.77688316
CCTATAC802.004801E-613.0591063
AGAACCG1800.012.6642885
GTCCTAA2350.012.5309171
GTATTAG2050.012.5111981
AAGACGG3050.012.45667655
CGAGCCG3250.012.27815715
AAGACCG701.0926514E-412.2119925
ACTAGTG550.003072479512.0886375
CGTTATT1501.4551915E-1112.0276052
CGACCAT1900.012.00077410
GTTATTC1900.011.9968813
CCCTATA951.0454896E-611.99428752
GTAGGAC5000.011.9668883
ACGCGCG802.8650044E-511.87533813
AACCGCG1603.6379788E-1211.8744827
AATCGAA1201.0013537E-811.8744817
ACTAGAC802.8734728E-511.8719143
AGGCCCG3450.011.84134310
CGGTCCA3050.011.83682910