##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064081_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1387939
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23297421572562	32.0	32.0	32.0	32.0	32.0
2	30.79795509745025	32.0	32.0	32.0	32.0	32.0
3	30.808757445392054	32.0	32.0	32.0	32.0	32.0
4	30.851133947529394	32.0	32.0	32.0	32.0	32.0
5	30.741010952210434	32.0	32.0	32.0	32.0	32.0
6	34.396983585013466	36.0	36.0	36.0	32.0	36.0
7	34.32116757292648	36.0	36.0	36.0	32.0	36.0
8	34.27290608593029	36.0	36.0	36.0	32.0	36.0
9	34.41781375118071	36.0	36.0	36.0	32.0	36.0
10	34.092718772222696	36.0	36.0	36.0	32.0	36.0
11	34.422224607853806	36.0	36.0	36.0	32.0	36.0
12	34.20122858425334	36.0	36.0	36.0	32.0	36.0
13	34.30787952496471	36.0	36.0	36.0	32.0	36.0
14	34.19314465549279	36.0	36.0	36.0	32.0	36.0
15	34.10825619857933	36.0	36.0	36.0	32.0	36.0
16	34.11848647527017	36.0	36.0	36.0	32.0	36.0
17	34.049639789644935	36.0	36.0	36.0	32.0	36.0
18	34.044757010214425	36.0	36.0	36.0	32.0	36.0
19	34.058911090472996	36.0	36.0	36.0	32.0	36.0
20	34.039918901335	36.0	36.0	36.0	32.0	36.0
21	34.017121069441814	36.0	36.0	36.0	32.0	36.0
22	33.9835727650855	36.0	36.0	36.0	32.0	36.0
23	33.93372619401861	36.0	36.0	36.0	32.0	36.0
24	33.9268591775287	36.0	36.0	36.0	32.0	36.0
25	33.51293968971259	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	12.0
5	19.0
6	60.0
7	19.0
8	81.0
9	77.0
10	127.0
11	46.0
12	72.0
13	65.0
14	141.0
15	224.0
16	372.0
17	509.0
18	654.0
19	881.0
20	1376.0
21	2090.0
22	3305.0
23	5242.0
24	7962.0
25	11539.0
26	16399.0
27	21669.0
28	29134.0
29	39532.0
30	51938.0
31	70350.0
32	99405.0
33	139998.0
34	294518.0
35	590116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58320365403702	17.21230476929098	11.41792868227335	25.786562894398646
2	16.73437301852642	19.61370632111689	37.57479162103273	26.077129039323964
3	18.809838087717377	23.80525566462427	28.51564076108572	28.869265486572637
4	12.617121414305025	15.248552464341195	35.90155028259009	36.23277583876369
5	14.67739250800367	36.57707409059368	33.42525333243023	15.320280068972425
6	34.28325189355589	35.4524969860917	16.82549885316556	13.438752267186842
7	30.491799261901143	30.393933781535132	20.700783140040603	18.413483816523126
8	28.278197293302014	32.7218985662155	19.324702132766152	19.67520200771633
9	27.53420934830858	14.063190431258004	18.50790078622212	39.8946994342113
10	16.13061591708473	26.28012379330008	31.283472383323808	26.305787906291382
11	37.27753107067678	20.968886069379163	22.601213991176216	19.15236886876784
12	25.12001891753354	23.527600926555415	28.577515386717213	22.774864769193833
13	29.490366508151194	19.335330878218013	25.380595745171718	25.793706868459076
14	23.663640020937063	19.74644812525324	25.02072798111627	31.569183872693422
15	25.130505056913254	27.443225524128927	22.168809850112932	25.257459568844887
16	25.706744415581795	25.617488891724687	23.94189306784501	24.733873624848506
17	24.022614201066546	25.956875278009477	25.11828694975751	24.902223571166463
18	24.997674611443795	24.696708042960203	26.315611108507316	23.990006237088686
19	25.646736485390342	24.775528597436775	25.40389711051427	24.173837806658614
20	25.99422866430064	24.051630524914664	24.915600344376553	25.038540466408143
21	27.00279106254331	23.964058670715907	24.327235680032185	24.7059145867086
22	26.173739302357607	24.113417902602333	24.891572467807027	24.82127032723303
23	24.336958967170276	24.11558474522495	25.618333535238165	25.929122752366613
24	24.891916290284435	24.79616234331828	25.347252265503776	24.96466910089351
25	24.833023403041814	24.500908880494293	25.356431852710166	25.309635863753734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	344.5
3	529.0
4	529.0
5	529.0
6	1388.5
7	2248.0
8	2248.0
9	2248.0
10	2370.0
11	2492.0
12	2492.0
13	2492.0
14	2944.0
15	3396.0
16	3396.0
17	3396.0
18	5765.0
19	8134.0
20	8134.0
21	8134.0
22	13707.0
23	19280.0
24	19280.0
25	19280.0
26	29324.5
27	39369.0
28	39369.0
29	39369.0
30	49276.0
31	59183.0
32	59183.0
33	59183.0
34	73397.5
35	87612.0
36	87612.0
37	87612.0
38	100337.0
39	113062.0
40	113062.0
41	113062.0
42	129846.5
43	146631.0
44	146631.0
45	146631.0
46	163366.5
47	180102.0
48	180102.0
49	180102.0
50	184008.0
51	187914.0
52	187914.0
53	187914.0
54	173869.0
55	159824.0
56	159824.0
57	159824.0
58	147165.0
59	134506.0
60	134506.0
61	134506.0
62	118483.5
63	102461.0
64	102461.0
65	102461.0
66	84431.0
67	66401.0
68	66401.0
69	66401.0
70	50328.5
71	34256.0
72	34256.0
73	34256.0
74	26761.0
75	19266.0
76	19266.0
77	19266.0
78	15573.0
79	11880.0
80	11880.0
81	11880.0
82	8156.5
83	4433.0
84	4433.0
85	4433.0
86	3441.5
87	2450.0
88	2450.0
89	2450.0
90	1780.0
91	1110.0
92	1110.0
93	1110.0
94	775.0
95	440.0
96	440.0
97	440.0
98	620.0
99	800.0
100	800.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039050707559914376
2	0.005980089903086519
3	0.0019453304504016388
4	0.004539104384270491
5	0.009942800079830597
6	0.014842150843805095
7	0.02305576830105646
8	0.03544824376287431
9	0.048417113432218564
10	0.056774829441351524
11	0.05612638595788432
12	0.06239467296473404
13	0.0650604961745437
14	0.06664558024524132
15	0.059656800478983585
16	0.06556484110612931
17	0.06095368744591801
18	0.07738092236042074
19	0.07305796580397265
20	0.07817346439576955
21	0.07291386725209105
22	0.07694862670477594
23	0.08264051950409924
24	0.07572378901378231
25	0.07694862670477594
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1387939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.790507558670576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14441276901135	43.911530536057306
2	12.069845653643414	13.226259390358733
3	3.402495049080227	5.592732921149082
4	1.4490777327379123	3.1758281787471123
5	0.7586927676261984	2.078458090966596
6	0.4751271319610708	1.5619474029025515
7	0.30537342525207556	1.171209547514368
8	0.21753464957100094	0.9535067089274145
9	0.16116477367018675	0.7947269774971021
>10	0.8507476424249564	8.37218986463076
>50	0.06883612684029643	2.6288278194157555
>100	0.08231378924756796	9.581743125767742
>500	0.010421106658392541	3.873614059682745
>1k	0.00395738227533894	3.077425376382715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3519	0.2535414020356802	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2515	0.1812039289911156	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1707	0.12298811403094806	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1617	0.11650367919627592	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1580	0.11383785598646627	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1573	0.11333351105488065	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1547	0.11146022988041981	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1508	0.10865030811872856	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1504	0.10836211101496536	No Hit
GAACTACGACGGTATCTGATCGTCT	1454	0.10475964721792527	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1423	0.10252611966376043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.161478278224043E-4
13	0.0	0.0	0.0	0.0	2.161478278224043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	200	0.0	13.776883	16
CCTATAC	80	2.004801E-6	13.059106	3
AGAACCG	180	0.0	12.664288	5
GTCCTAA	235	0.0	12.530917	1
GTATTAG	205	0.0	12.511198	1
AAGACGG	305	0.0	12.4566765	5
CGAGCCG	325	0.0	12.278157	15
AAGACCG	70	1.0926514E-4	12.211992	5
ACTAGTG	55	0.0030724795	12.088637	5
CGTTATT	150	1.4551915E-11	12.027605	2
CGACCAT	190	0.0	12.000774	10
GTTATTC	190	0.0	11.996881	3
CCCTATA	95	1.0454896E-6	11.9942875	2
GTAGGAC	500	0.0	11.966888	3
ACGCGCG	80	2.8650044E-5	11.875338	13
AACCGCG	160	3.6379788E-12	11.874482	7
AATCGAA	120	1.0013537E-8	11.874481	7
ACTAGAC	80	2.8734728E-5	11.871914	3
AGGCCCG	345	0.0	11.841343	10
CGGTCCA	305	0.0	11.836829	10
>>END_MODULE