FastQCFastQC Report
Thu 2 Feb 2017
SRR4064081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387939
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17840.12853590827838976No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16890.12169122706401363No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16830.12125893140836881No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15180.10937080087813657No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15080.10865030811872856No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14380.10360685880287247No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14350.10339071097505005No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14230.10252611966376043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG250.002353139235.199315
GACGGGT500.002580922821.9995697
GTATCAA27900.021.5280111
GTCCTAC4450.020.7651351
TAGCCCG550.004484451419.9996075
TAGGACG3400.019.4113834
TCCTACA5550.019.0266532
GATATAC2350.018.7243861
GTCCTAG2250.017.6009221
AACGCAG33950.017.4311756
CGACGGT4300.017.3950067
TTAGGAC2800.017.2847543
TACGACG4500.017.1107755
ACGACGG4400.016.9996666
TTAGTAC1307.3229785E-716.9221343
CGAGCCG4950.016.88855615
GTAGGAC5300.016.6028483
CGTCGTA4000.016.5002710
ATAGTAC1206.411303E-616.4990833
TGTAGGA5700.016.2102072
Produced by FastQC (version 0.11.5)