##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1387939
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324234710603275	32.0	32.0	32.0	32.0	32.0
2	31.427028853573535	32.0	32.0	32.0	32.0	32.0
3	31.4898968902812	32.0	32.0	32.0	32.0	32.0
4	31.57318225080497	32.0	32.0	32.0	32.0	32.0
5	31.523381791274687	32.0	32.0	32.0	32.0	32.0
6	35.11756856749468	36.0	36.0	36.0	36.0	36.0
7	35.134750878821045	36.0	36.0	36.0	36.0	36.0
8	35.08138614160997	36.0	36.0	36.0	36.0	36.0
9	35.17672966895519	36.0	36.0	36.0	36.0	36.0
10	35.03985837994321	36.0	36.0	36.0	36.0	36.0
11	35.183241482514724	36.0	36.0	36.0	36.0	36.0
12	35.087425311919326	36.0	36.0	36.0	36.0	36.0
13	35.129993465130674	36.0	36.0	36.0	36.0	36.0
14	35.072420329711896	36.0	36.0	36.0	36.0	36.0
15	35.04620303918256	36.0	36.0	36.0	36.0	36.0
16	35.06045294497813	36.0	36.0	36.0	36.0	36.0
17	35.020330144192215	36.0	36.0	36.0	36.0	36.0
18	35.014551071769006	36.0	36.0	36.0	36.0	36.0
19	35.01068130515822	36.0	36.0	36.0	36.0	36.0
20	35.012548822390606	36.0	36.0	36.0	36.0	36.0
21	35.00168667354978	36.0	36.0	36.0	36.0	36.0
22	34.98815509903533	36.0	36.0	36.0	36.0	36.0
23	34.938602489014286	36.0	36.0	36.0	36.0	36.0
24	34.9099153493057	36.0	36.0	36.0	32.0	36.0
25	34.88265334427522	36.0	36.0	36.0	32.0	36.0
26	34.82368317339595	36.0	36.0	36.0	32.0	36.0
27	34.816427811308714	36.0	36.0	36.0	32.0	36.0
28	34.79558899922835	36.0	36.0	36.0	32.0	36.0
29	34.75185076577573	36.0	36.0	36.0	32.0	36.0
30	34.73099970531846	36.0	36.0	36.0	32.0	36.0
31	34.739639133996526	36.0	36.0	36.0	32.0	36.0
32	34.69487563934726	36.0	36.0	36.0	32.0	36.0
33	34.67636113690875	36.0	36.0	36.0	32.0	36.0
34	34.66633187769779	36.0	36.0	36.0	32.0	36.0
35	34.62413693973583	36.0	36.0	36.0	32.0	36.0
36	34.59364424517216	36.0	36.0	36.0	32.0	36.0
37	34.58906191122232	36.0	36.0	36.0	32.0	36.0
38	34.54354478114672	36.0	36.0	36.0	32.0	36.0
39	34.53316752393297	36.0	36.0	36.0	32.0	36.0
40	34.50816354321047	36.0	36.0	36.0	32.0	36.0
41	34.499122079572665	36.0	36.0	36.0	32.0	36.0
42	34.42191119350346	36.0	36.0	36.0	32.0	36.0
43	34.42584148150603	36.0	36.0	36.0	32.0	36.0
44	34.35175393154886	36.0	36.0	36.0	32.0	36.0
45	34.3186573761527	36.0	36.0	36.0	32.0	36.0
46	34.292246993563836	36.0	36.0	36.0	32.0	36.0
47	34.25808050641995	36.0	36.0	36.0	32.0	36.0
48	34.21471116526015	36.0	36.0	36.0	32.0	36.0
49	34.19436517022722	36.0	36.0	36.0	32.0	36.0
50	33.59206996849285	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	3.0
20	8.0
21	52.0
22	133.0
23	397.0
24	1172.0
25	2648.0
26	5247.0
27	9569.0
28	15763.0
29	24224.0
30	35058.0
31	49335.0
32	70799.0
33	113941.0
34	238583.0
35	821002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.001749515244484	18.00357829188803	11.836969389968246	26.157702802899234
2	16.0233354371828	20.472688863719547	36.97299291319748	26.530982785900175
3	18.054028505678225	24.393378935886002	28.746179573380292	28.806412985055484
4	12.232742217057089	15.940181809142908	35.89956042736748	35.92751554643252
5	14.23030839251581	36.98210079837803	33.28654933682244	15.501041472283724
6	34.37574300869347	35.7818576910496	16.575091970967005	13.267307329289926
7	30.46733321853482	30.504726792748095	20.721083563470728	18.306856425246355
8	28.281430235766848	32.95000716890296	19.15415591031018	19.61440668502002
9	27.653290756370335	13.88705961014988	18.377670726944963	40.08197890653483
10	16.11919629894613	26.48665745870286	31.26564831486337	26.128497927487643
11	37.49833386049387	20.898540930112922	22.51467823874104	19.088446970652168
12	25.07053639360893	23.623711756161665	28.5197588361423	22.785993014087104
13	29.601399493202475	19.288062591007765	25.405081376523757	25.705456539266002
14	23.79036209162382	19.75948458718558	24.980834851174695	31.46931847001591
15	25.134305946640765	27.520974496280648	22.089293279803098	25.25542627727549
16	25.841921080863763	25.65776153933168	23.77769147801001	24.722625901794544
17	24.13542670102937	26.11339547343219	25.006286299325836	24.744891526212605
18	25.07576008657472	24.646200755943724	26.33338472866865	23.944654428812896
19	25.759291841566412	24.783095498502096	25.31827790578542	24.139334754146077
20	26.136908921919694	24.039901147752406	24.896068245516705	24.927121684811194
21	27.17648253993871	23.945288661821593	24.302653070487967	24.575575727751726
22	26.304489105421137	24.1798264763988	24.854208185091842	24.661476233088223
23	24.401696346485828	24.119908582890705	25.57128488303394	25.90711018758952
24	24.953077706200517	24.894429342050373	25.24812076663643	24.904372185112685
25	24.96628018571836	24.353199203405413	25.399666260299675	25.280854350576554
26	24.624025478074156	25.381450578588616	25.761243929502974	24.23328001383425
27	25.46469727396333	24.683050411467036	24.81973773614951	25.032514578420116
28	24.536824547056092	24.703555461085912	25.734275307756878	25.02534468410112
29	24.67632684558004	24.70312998731174	25.564793352820853	25.05574981428737
30	24.526960086750705	24.916977991692395	25.784485025776632	24.77157689578027
31	25.364669498901883	24.61349788735236	24.679644222067225	25.342188391678533
32	25.01664389839008	24.8597877650775	24.539807017240484	25.583761319291938
33	24.459064727777882	24.47952768046667	25.38306791538713	25.678339676368317
34	25.327976574748686	24.525903808908975	25.434828345476348	24.711291270865996
35	25.858133462449633	24.465954221353762	25.389078144183713	24.286834172012888
36	24.608002859141866	25.13515714111753	25.07246930277433	25.18437069696627
37	25.656160272645067	24.889237941183886	24.718906825853725	24.735694960317318
38	24.754688974874444	24.72111137531254	25.333290100372523	25.190909549440494
39	25.66117265603891	24.474034044852743	24.5976763037017	25.267116995406646
40	25.76394137622208	24.722944936274274	25.17117575389023	24.34193793361342
41	24.678956261100442	25.193980812669547	25.61526628455323	24.511796641676785
42	26.00434897263087	25.346239032101415	24.836409172326242	23.813002822941478
43	25.05218259043241	24.387732678153924	25.31732851075174	25.242756220661928
44	24.75086730815648	25.043032174160523	24.836319227042004	25.369781290640997
45	24.809408631784176	25.349282413861925	24.88607027653671	24.95523867781719
46	24.67573826194844	24.782156345961805	25.108183312895104	25.433922079194655
47	25.085182711422082	24.565980665740085	25.465181544190173	24.883655078647653
48	25.696100110165567	25.32806498399393	24.307826654590965	24.66800825124954
49	24.859179182871806	25.464467330579122	24.512837065739436	25.163516420809636
50	24.828952677387743	25.790679578073668	24.464594501123983	24.915773243414606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	33.5
2	23.0
3	87.0
4	151.0
5	193.0
6	235.0
7	235.0
8	235.0
9	297.5
10	360.0
11	565.5
12	771.0
13	1434.5
14	2098.0
15	2772.5
16	3447.0
17	3672.0
18	3897.0
19	3874.5
20	3852.0
21	4286.5
22	4721.0
23	5378.5
24	6036.0
25	6874.5
26	7713.0
27	9507.5
28	11302.0
29	13613.5
30	15925.0
31	18835.5
32	21746.0
33	25517.5
34	29289.0
35	34386.0
36	39483.0
37	46186.5
38	52890.0
39	56972.5
40	61055.0
41	66184.5
42	71314.0
43	73174.5
44	75035.0
45	82722.0
46	90409.0
47	96156.5
48	101904.0
49	105855.0
50	109806.0
51	105142.5
52	100479.0
53	99295.0
54	98111.0
55	98792.0
56	99473.0
57	96959.0
58	94445.0
59	86185.0
60	77925.0
61	68177.5
62	58430.0
63	50984.0
64	43538.0
65	37219.5
66	30901.0
67	26708.5
68	22516.0
69	20632.0
70	18748.0
71	14353.5
72	9959.0
73	8666.5
74	7374.0
75	5775.5
76	4177.0
77	3674.0
78	3171.0
79	2614.0
80	2057.0
81	1672.5
82	1288.0
83	1071.0
84	854.0
85	606.5
86	359.0
87	265.0
88	171.0
89	129.0
90	87.0
91	70.5
92	54.0
93	36.0
94	18.0
95	27.0
96	36.0
97	25.0
98	14.0
99	13.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009078208768540982
2	5.043449315856101E-4
3	3.6024637970400716E-4
4	0.0
5	0.0
6	7.204927594080143E-5
7	0.0
8	0.0
9	0.0015130347947568301
10	7.204927594080143E-4
11	0.0
12	2.161478278224043E-4
13	5.763942075264115E-4
14	2.161478278224043E-4
15	0.004539104384270491
16	8.645913112896172E-4
17	0.0
18	3.6024637970400716E-4
19	7.204927594080143E-5
20	6.484434834672129E-4
21	0.0
22	7.204927594080143E-5
23	0.0010807391391120214
24	4.322956556448086E-4
25	0.002233527554164844
26	0.005691892799323313
27	0.0072049275940801434
28	0.005331646419619306
29	0.0030260695895136603
30	0.0038906609008032777
31	0.007709272525665754
32	0.0030981188654544615
33	0.004683202936152093
34	0.00252172465792805
35	0.0030981188654544615
36	0.007925420353488158
37	0.0038906609008032777
38	0.007853371077547357
39	0.004611153660211292
40	0.0027378724857504548
41	0.003170168141395263
42	0.0018012318985200359
43	0.0017291826225792344
44	0.0017291826225792344
45	0.0015850840706976314
46	0.0012248376909936244
47	0.0028819710376320573
48	0.0020173797263424403
49	3.6024637970400716E-4
50	0.0013689362428752274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1387939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.20202605487689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07510972841916	47.18735648815455
2	11.690670194596366	13.608413825297408
3	3.2457031933878935	5.66719505483878
4	1.320242801439475	3.073632237125759
5	0.7186143819358133	2.091240649041873
6	0.4234505497877362	1.4787407959018661
7	0.2790540938970452	1.1369059550601124
8	0.2009268498975022	0.9355479802287009
9	0.15513220271655098	0.8126107654013229
>10	0.7477363997066656	7.664760642067807
>50	0.06813715300851907	2.788403103312589
>100	0.06413838095101249	8.18094676347898
>500	0.008717808066634984	3.527621183833581
>1k	0.00236626218951521	1.8466245562566899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1784	0.12853590827838976	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1689	0.12169122706401363	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1683	0.12125893140836881	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1518	0.10937080087813657	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1508	0.10865030811872856	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1438	0.10360685880287247	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1435	0.10339071097505005	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1423	0.10252611966376043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	7.204927594080143E-5	0.0	0.0	0.0
4	0.0	7.204927594080143E-5	0.0	0.0	0.0
5	0.0	7.204927594080143E-5	0.0	0.0	0.0
6	0.0	7.204927594080143E-5	0.0	0.0	0.0
7	0.0	7.204927594080143E-5	0.0	0.0	0.0
8	0.0	7.204927594080143E-5	0.0	0.0	0.0
9	0.0	7.204927594080143E-5	0.0	0.0	7.204927594080143E-5
10	0.0	7.204927594080143E-5	0.0	0.0	7.204927594080143E-5
11	0.0	7.204927594080143E-5	0.0	0.0	7.204927594080143E-5
12	0.0	7.204927594080143E-5	0.0	0.0	7.204927594080143E-5
13	0.0	7.204927594080143E-5	0.0	0.0	2.8819710376320573E-4
14	0.0	7.204927594080143E-5	0.0	0.0	2.8819710376320573E-4
15	0.0	7.204927594080143E-5	0.0	0.0	2.8819710376320573E-4
16	0.0	7.204927594080143E-5	0.0	0.0	3.6024637970400716E-4
17	0.0	7.204927594080143E-5	0.0	7.204927594080143E-5	3.6024637970400716E-4
18	0.0	7.204927594080143E-5	0.0	2.161478278224043E-4	3.6024637970400716E-4
19	0.0	7.204927594080143E-5	0.0	2.161478278224043E-4	3.6024637970400716E-4
20	0.0	7.204927594080143E-5	0.0	3.6024637970400716E-4	3.6024637970400716E-4
21	0.0	7.204927594080143E-5	0.0	5.043449315856101E-4	4.322956556448086E-4
22	0.0	7.204927594080143E-5	0.0	7.204927594080143E-4	5.043449315856101E-4
23	0.0	7.204927594080143E-5	0.0	0.0012248376909936244	5.043449315856101E-4
24	0.0	7.204927594080143E-5	0.0	0.0017291826225792344	5.763942075264115E-4
25	0.0	7.204927594080143E-5	0.0	0.0020173797263424403	5.763942075264115E-4
26	0.0	7.204927594080143E-5	0.0	0.002377626106046447	6.484434834672129E-4
27	0.0	7.204927594080143E-5	0.0	0.002809921761691256	6.484434834672129E-4
28	0.0	7.204927594080143E-5	0.0	0.005115498591796902	7.204927594080143E-4
29	0.0	7.204927594080143E-5	0.0	0.008790011664777775	7.204927594080143E-4
30	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.017291826225792343	7.204927594080143E-4
31	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.04193267859754644	7.204927594080143E-4
32	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.06686172807306373	7.204927594080143E-4
33	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.08919700361471218	7.204927594080143E-4
34	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.11419810236617027	7.925420353488158E-4
35	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.1456115866763597	7.925420353488158E-4
36	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.18776041310172853	7.925420353488158E-4
37	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.25375754986350263	7.925420353488158E-4
38	1.4409855188160286E-4	7.204927594080143E-5	0.0	0.33877569547364833	7.925420353488158E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	25	0.0023531392	35.19931	5
GACGGGT	50	0.0025809228	21.999569	7
GTATCAA	2790	0.0	21.528011	1
GTCCTAC	445	0.0	20.765135	1
TAGCCCG	55	0.0044844514	19.999607	5
TAGGACG	340	0.0	19.411383	4
TCCTACA	555	0.0	19.026653	2
GATATAC	235	0.0	18.724386	1
GTCCTAG	225	0.0	17.600922	1
AACGCAG	3395	0.0	17.431175	6
CGACGGT	430	0.0	17.395006	7
TTAGGAC	280	0.0	17.284754	3
TACGACG	450	0.0	17.110775	5
ACGACGG	440	0.0	16.999666	6
TTAGTAC	130	7.3229785E-7	16.922134	3
CGAGCCG	495	0.0	16.888556	15
GTAGGAC	530	0.0	16.602848	3
CGTCGTA	400	0.0	16.50027	10
ATAGTAC	120	6.411303E-6	16.499083	3
TGTAGGA	570	0.0	16.210207	2
>>END_MODULE