FastQCFastQC Report
Thu 2 Feb 2017
SRR4064080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031634
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54020.5236353202783157No Hit
TATCAACGCAGAGTACTTTTTTTTT38940.3774594478274272No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37710.3655366147296425No Hit
GTACATGGGGTGGTATCAACGCAAA25260.2448542797154805No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24610.23855359555811462No Hit
GTACATGGAAGCAGTGGTATCAACG24190.23448238425643203No Hit
TCCATGTACTCTGCGTTGATACCAC21210.20559617073496994No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19990.19377027123960627No Hit
GGTATCAACGCAGAGTACTTTTTTT17400.16866446821256376No Hit
GCCTAGTACCCTGGAAACGGTTCGT14430.13987518829352272No Hit
GTATCAACGCAGAGTACATGGGGTG14190.13754878183541838No Hit
GTACATGGGTGGTATCAACGCAAAA13000.12601368314731776No Hit
CCCATGTACTCTGCGTTGATACCAC11520.11166750998900772No Hit
GTACATGGGAAGCAGTGGTATCAAC10670.10342815378322158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT250.00603081818.9999521
GCTCGTA704.4245826E-714.9307069
TATTAGC704.4636545E-714.9191242
GTATAGA707.2570638E-613.5713941
ACTAGGC2100.013.5648143
CGCATCG1205.0931703E-1113.45829913
TACTCCG500.001502269813.2960975
CGACCAT655.4441873E-513.15508810
TTAGAGT1403.6379788E-1212.8871974
AATCGTA604.090038E-412.66663413
TACTAGG2100.012.6586512
CGCAATA604.111851E-412.6586492
GTATTAG1001.4406214E-712.3499691
CCGTTAT701.0887591E-412.2160329
GCATCGC1405.2750693E-1112.21603214
TGGTACG1251.4060788E-912.16114910
AGAATTT1650.012.09381117
TGATACT550.003077343812.0856014
TAGGCTG2600.012.05415255
CGTCGTA951.0374479E-612.00113410
Produced by FastQC (version 0.11.5)