##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064080_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031634
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161205427506268	32.0	32.0	32.0	32.0	32.0
2	30.77606980770312	32.0	32.0	32.0	32.0	32.0
3	30.807503436296205	32.0	32.0	32.0	32.0	32.0
4	30.826503391706748	32.0	32.0	32.0	32.0	32.0
5	30.74755484987893	32.0	32.0	32.0	32.0	32.0
6	34.37689238625327	36.0	36.0	36.0	32.0	36.0
7	34.28200796018743	36.0	36.0	36.0	32.0	36.0
8	34.23008450671459	36.0	36.0	36.0	32.0	36.0
9	34.35969054916763	36.0	36.0	36.0	32.0	36.0
10	34.085728077981145	36.0	36.0	36.0	32.0	36.0
11	34.399721218959435	36.0	36.0	36.0	32.0	36.0
12	34.192304635171	36.0	36.0	36.0	32.0	36.0
13	34.26451435295851	36.0	36.0	36.0	32.0	36.0
14	34.17162288175845	36.0	36.0	36.0	32.0	36.0
15	34.129125251785034	36.0	36.0	36.0	32.0	36.0
16	34.10207883803752	36.0	36.0	36.0	32.0	36.0
17	34.03413710676461	36.0	36.0	36.0	32.0	36.0
18	34.027030904371124	36.0	36.0	36.0	32.0	36.0
19	34.03268988808046	36.0	36.0	36.0	32.0	36.0
20	33.988862329081826	36.0	36.0	36.0	32.0	36.0
21	33.95236585843429	36.0	36.0	36.0	32.0	36.0
22	33.96504283495891	36.0	36.0	36.0	32.0	36.0
23	33.91841680285838	36.0	36.0	36.0	32.0	36.0
24	33.88321827314726	36.0	36.0	36.0	32.0	36.0
25	33.5130647109343	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	7.0
5	16.0
6	47.0
7	15.0
8	70.0
9	78.0
10	95.0
11	23.0
12	63.0
13	54.0
14	130.0
15	208.0
16	300.0
17	405.0
18	651.0
19	800.0
20	1269.0
21	1906.0
22	2847.0
23	4401.0
24	6414.0
25	9234.0
26	12905.0
27	16608.0
28	22217.0
29	29316.0
30	37887.0
31	51134.0
32	71864.0
33	99767.0
34	210196.0
35	450702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.16770539287512	16.68733775458651	11.193321379981787	24.951635472556585
2	16.510157807848415	19.636845161409152	38.209488246567616	25.643508784174816
3	19.004672263042593	25.06930846629573	28.321474961710706	27.604544308950967
4	12.305822160483833	15.044111388890775	35.94896436869967	36.70110208192572
5	13.907830473837993	37.560952910907446	33.379122743409326	15.152093871845231
6	32.97117080259702	36.13611003122671	17.340272169649896	13.55244699652638
7	30.053714440846246	30.91013981267816	20.547227985805424	18.48891776067017
8	29.032573997432248	32.48924608146215	18.923502693812956	19.55467722729265
9	28.343189872042796	13.479729926313563	18.092551249798763	40.08452895184488
10	15.992058527141934	26.628472333355642	31.36193155862559	26.017537580876844
11	37.384306069185044	20.907201416792994	22.114210507428755	19.594282006593208
12	25.209168698702015	23.669962540713453	29.288564539188698	21.83230422139583
13	30.249148634663154	19.927332052365152	25.40565339561645	24.417865917355243
14	23.95783318929185	19.773937318190065	25.48603088598607	30.782198606532013
15	25.67967069312347	26.96558921605332	23.105602833715157	24.24913725710805
16	25.384988457136203	25.993559276000543	24.564766135759598	24.056686131103653
17	23.185891007000954	25.847670518582895	25.903538499440348	25.0628999749758
18	23.97248114917976	25.3693771299026	27.28069945685968	23.37744226405796
19	26.03038180971597	25.030653422318792	25.616948626416264	23.322016141548968
20	25.943006814929788	24.377464652099047	26.012756772488054	23.66677176048311
21	26.65811723712444	23.527066510940294	25.21069622880912	24.604120023126143
22	25.743349362316785	24.526672881656822	25.464547878867837	24.265429877158553
23	24.317056252698162	24.63467517789667	25.98198477873874	25.06628379066643
24	25.186329167462944	25.29177745851187	25.67137190457851	23.850521469446672
25	24.72468947533846	24.89076826963746	25.558187723362398	24.82635453166168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	205.0
2	561.0
3	917.0
4	917.0
5	917.0
6	2712.0
7	4507.0
8	4507.0
9	4507.0
10	4131.0
11	3755.0
12	3755.0
13	3755.0
14	3143.0
15	2531.0
16	2531.0
17	2531.0
18	4457.0
19	6383.0
20	6383.0
21	6383.0
22	10574.0
23	14765.0
24	14765.0
25	14765.0
26	23193.5
27	31622.0
28	31622.0
29	31622.0
30	41675.5
31	51729.0
32	51729.0
33	51729.0
34	58609.5
35	65490.0
36	65490.0
37	65490.0
38	72211.5
39	78933.0
40	78933.0
41	78933.0
42	92618.5
43	106304.0
44	106304.0
45	106304.0
46	126108.0
47	145912.0
48	145912.0
49	145912.0
50	142181.0
51	138450.0
52	138450.0
53	138450.0
54	126119.5
55	113789.0
56	113789.0
57	113789.0
58	102471.0
59	91153.0
60	91153.0
61	91153.0
62	80080.0
63	69007.0
64	69007.0
65	69007.0
66	58362.0
67	47717.0
68	47717.0
69	47717.0
70	36171.0
71	24625.0
72	24625.0
73	24625.0
74	19623.0
75	14621.0
76	14621.0
77	14621.0
78	12325.0
79	10029.0
80	10029.0
81	10029.0
82	7195.0
83	4361.0
84	4361.0
85	4361.0
86	3400.5
87	2440.0
88	2440.0
89	2440.0
90	1952.0
91	1464.0
92	1464.0
93	1464.0
94	877.0
95	290.0
96	290.0
97	290.0
98	462.5
99	635.0
100	635.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0390642417756685
2	0.00610681695252386
3	0.0013570704338941911
4	0.00397427769926156
5	0.009693360242101365
6	0.01405537235104698
7	0.02462113501493747
8	0.037028636124827215
9	0.04924227002987494
10	0.05728775903081908
11	0.055542954187240826
12	0.06281297436881685
13	0.06232830635671178
14	0.0660117832487103
15	0.06009883350102847
16	0.06727192008018348
17	0.061262036730080634
18	0.0753174090811276
19	0.0730879362254443
20	0.07841928435860004
21	0.07425113945449646
22	0.07686834671986384
23	0.08132729243123045
24	0.07725608112954789
25	0.07735301473196889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1031634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.75726696916761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53033743492529	35.11607955212706
2	14.972743608025823	14.301146274987744
3	5.088864859601177	7.290908330099671
4	2.2493805917363283	4.296970777392643
5	1.1677733240545956	2.78848311981738
6	0.696046463782668	1.9944766076288356
7	0.4485994211631897	1.4996717623093156
8	0.2871767808511562	1.0971822552363848
9	0.218626170313218	0.9396889543885552
>10	1.0905980371409243	9.817668032994648
>50	0.12625978601747137	4.230462181282911
>100	0.11113202691277164	10.32955054542606
>500	0.00939719330927849	3.0072448537259775
>1k	0.002860015354997801	2.763437921158458
>5k	2.0428681107127153E-4	0.5270288314244657
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5402	0.5236353202783157	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3894	0.3774594478274272	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3771	0.3655366147296425	No Hit
GTACATGGGGTGGTATCAACGCAAA	2526	0.2448542797154805	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2461	0.23855359555811462	No Hit
GTACATGGAAGCAGTGGTATCAACG	2419	0.23448238425643203	No Hit
TCCATGTACTCTGCGTTGATACCAC	2121	0.20559617073496994	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1999	0.19377027123960627	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1740	0.16866446821256376	No Hit
GCCTAGTACCCTGGAAACGGTTCGT	1443	0.13987518829352272	No Hit
GTATCAACGCAGAGTACATGGGGTG	1419	0.13754878183541838	No Hit
GTACATGGGTGGTATCAACGCAAAA	1300	0.12601368314731776	No Hit
CCCATGTACTCTGCGTTGATACCAC	1152	0.11166750998900772	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1067	0.10342815378322158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.693360242101366E-5	0.0	0.0	0.0
3	0.0	9.693360242101366E-5	0.0	0.0	0.0
4	0.0	9.693360242101366E-5	0.0	0.0	0.0
5	0.0	9.693360242101366E-5	0.0	0.0	0.0
6	0.0	9.693360242101366E-5	0.0	0.0	0.0
7	0.0	3.877344096840546E-4	0.0	0.0	0.0
8	0.0	3.877344096840546E-4	0.0	0.0	0.0
9	0.0	3.877344096840546E-4	0.0	0.0	0.0
10	0.0	3.877344096840546E-4	0.0	0.0	0.0
11	0.0	3.877344096840546E-4	0.0	0.0	9.693360242101366E-5
12	0.0	3.877344096840546E-4	0.0	0.0	1.938672048420273E-4
13	0.0	3.877344096840546E-4	0.0	9.693360242101366E-5	1.938672048420273E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	25	0.006030818	18.999952	1
GCTCGTA	70	4.4245826E-7	14.930706	9
TATTAGC	70	4.4636545E-7	14.919124	2
GTATAGA	70	7.2570638E-6	13.571394	1
ACTAGGC	210	0.0	13.564814	3
CGCATCG	120	5.0931703E-11	13.458299	13
TACTCCG	50	0.0015022698	13.296097	5
CGACCAT	65	5.4441873E-5	13.155088	10
TTAGAGT	140	3.6379788E-12	12.887197	4
AATCGTA	60	4.090038E-4	12.666634	13
TACTAGG	210	0.0	12.658651	2
CGCAATA	60	4.111851E-4	12.658649	2
GTATTAG	100	1.4406214E-7	12.349969	1
CCGTTAT	70	1.0887591E-4	12.216032	9
GCATCGC	140	5.2750693E-11	12.216032	14
TGGTACG	125	1.4060788E-9	12.161149	10
AGAATTT	165	0.0	12.093811	17
TGATACT	55	0.0030773438	12.085601	4
TAGGCTG	260	0.0	12.0541525	5
CGTCGTA	95	1.0374479E-6	12.001134	10
>>END_MODULE