FastQCFastQC Report
Thu 2 Feb 2017
SRR4064080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031634
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15650.15170108778888636No Hit
GCCTAGTACCCTGGAAACGGTTCGTCCGGAGATAGGGTCGGACGGCTGGA14420.1397782546911017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.1327021017143677No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11890.11525405327858523No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10610.10284655216869548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1400.026.71366910
TACGTCC752.0659354E-623.463856
TAGGCTG3550.021.6875365
ACTAGGC3250.021.6578883
ACCGTCG1750.021.3688628
ATACCGT1750.021.3688626
GTCGTAG2000.020.89850211
GATATAC3000.020.5338541
TTATTCG755.470569E-520.53385422
AACGGTT3550.020.45023216
ACGTATG654.9258117E-420.31116132
ACGTTGC654.9322576E-420.30722215
GTACTAG3600.020.1671771
CGGTTTA550.004478744720.00341631
GCCTAGT3850.020.0005051
CCTAGTA4350.019.215152
AAACGGT3900.019.17904315
CGGTTCG4050.019.01098418
ACTGTTC3750.018.771088
CCGTCGT1901.8189894E-1218.5249889
Produced by FastQC (version 0.11.5)